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Application of guaiol in preparing drug for inhibiting tumor related M2 macrophage

A macrophage, tumor-related technology, applied in the field of new indications of drugs, to achieve the effect of inhibiting tumor growth and great medicinal prospects

Active Publication Date: 2019-07-05
SHANGHAI HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is no report on the application of guaiacol in inhibiting tumor-associated M2 macrophages.

Method used

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  • Application of guaiol in preparing drug for inhibiting tumor related M2 macrophage
  • Application of guaiol in preparing drug for inhibiting tumor related M2 macrophage
  • Application of guaiol in preparing drug for inhibiting tumor related M2 macrophage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 In vitro study of the inhibitory effect of guaiacol on the phenotype of M2 macrophages

[0044] 1 Establishment of M2 macrophage model in vitro

[0045] Mouse bone marrow-derived macrophages (Bone marrow-derived macrophage, BMDM) were used. Take a healthy C57BL / 6 male mouse, 6-8 weeks old, weighing 18-22g, kill the mouse cervical vertebrae, disinfect the abdominal skin of the mouse with 75% alcohol, and then make it supine, with its limbs spread out, and fix it in an anatomical board. Open the abdominal cavity of the mouse under aseptic conditions, take the femur, remove the muscle, cut off both ends with ophthalmological scissors, use a syringe to absorb DMEM culture solution containing 10% FBS, blow out the bone marrow cells, repeatedly blow and blow with a pipette gun, and use a pore size of 100um Filter through a filter to remove impurities, centrifuge at 300g for 10 minutes, and the resulting pellet is mouse bone marrow mononuclear cells. Inoculate the ...

Embodiment 2

[0054] Example 2 Inhibitory effect of guaiacol on subcutaneous xenograft tumors and spleen M2 macrophages in mice with Lewis lung cancer

[0055] 1 Experimental process

[0056] 1.1 Establishment of macrophage depletion mouse model and group administration

[0057] Take clean healthy male C57BL / 6 mice and divide them into 4 groups at random: Liposome PBS-normal saline group (M+ / Control), Liposome PBS-guaiacol group (M+ / Guaiol), Liposome Clodronate-normal saline Group (M- / Control), Liposome Clodronate-Guaiol group (M- / Guaiol), LLC cells were inoculated subcutaneously in the right scapular part of C57BL / 6 mice, and the inoculated cell density was 1×10 6 One / 0.2mL / one to establish a subcutaneous xenograft tumor model. Two days before inoculation of LLC cells, the four groups of mice were injected with macrophage scavenger (Liposome Clodronate / Liposome PBS) correspondingly, 0.2ml / mouse, and Liposome Clodronate / LiposomePBS was injected intraperitoneally every 4 days from day 0. ...

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Abstract

The invention relates to the technical field of new complications of drug, in particular to application of guaiol in preparing drug for inhibiting tumor related M2 macrophage, and discloses the drug for inhibiting the tumor related M2 macrophage. Experiments find that guaiol can modify M2 macrophage phenotype and promote conversion of the M2 macrophage into macrophage which is not activated by inducing, has obvious inhibiting effect on expression of macrophage surface marker, expression of cell factors secreted by the macrophage, expression of mRNA of the M2 macrophage and expression of M2 macrophage protein and achieves the objective of inhibiting tumor growth, invasion and metastasis by inhibiting the M2 macrophage so as to realize tumor treatment. Guaiol serving as a low-toxicity high-efficiency antitumor substance has great medicinal prospect, and a new idea is provided for tumor treatment.

Description

technical field [0001] The invention relates to the technical field of new indications of medicines, in particular to the application of guaiacol in the preparation of medicines for inhibiting tumor-associated M2 macrophages. Background technique [0002] Tumor is a kind of disease that seriously threatens human health. It and cardiovascular disease have become the top two causes of death worldwide. In tumor tissue, there are more non-tumor cells, including stromal cells (fibroblasts and endothelial cells) and leukocytes. Macrophages are a major component of leukocytes that infiltrate tumors. The relationship between tumor-associated macrophages (Tumor-associated macrophages, TAMs) in tumor tissues and their various functions in tumors are being studied in depth. The different activation states of tumor-associated macrophages determine whether they play anti-tumor or pro-tumor effects in tumors. On the one hand, it can exert immune surveillance and anti-tumor effects by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/045A61P35/00
CPCA61K31/045A61P35/00
Inventor 李雁吴建春曹亚娟骆莹滨
Owner SHANGHAI HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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