Sterilization method of coconut inflorescences

A sterilization method and inflorescence technology, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of increased test costs, cumbersome processing steps, and damage to explants, and achieve good disinfection and sterilization effects. Sterilization efficiency and damage reduction effect

Inactive Publication Date: 2019-07-09
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, chemical reagents will cause environmental pollution, material pollution, explant damage, explant browning, explant death, and increased test costs
On the other hand, in the test process, it is often necessary to soak the explants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sterilization method of coconut inflorescences
  • Sterilization method of coconut inflorescences
  • Sterilization method of coconut inflorescences

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0045] Example 1

[0046] A method for sterilizing coconut inflorescences, including the following steps: selecting unopened coconut inflorescences with buds, placing them in a tray, spraying evenly with 70% ethanol on the incisions of the coconut inflorescences and igniting them. After the flame is extinguished, spray 70% ethanol evenly on the incision of the coconut inflorescence and ignite it again. Repeat this 4 times. The amount of ethanol sprayed is 18ml each time. Sterilize the flower spikes inside the coconut inflorescence and wait until the flame is extinguished. After that, the buddha buds are cut open, and the inner buds are taken out for inoculation test.

Example Embodiment

[0047] Example 2

[0048] A method for sterilizing coconut inflorescence, including the following steps:

[0049] A. Pre-sterilization:

[0050] Select the unopened coconut inflorescences with buddha buds, wash them with 73% ethanol for 1.5 minutes, soak them in an antioxidant solution for 20 minutes, and wash them again with 73% ethanol to remove the residual antioxidant solution, and use Sterile air drying; among them, the antioxidant solution is a mixture of 1g / L citric acid and 1g / L ascorbic acid;

[0051] B. Dry and sterilize:

[0052] (1) Put the pre-sterilized coconut inflorescence in the tray;

[0053] (2) Evenly spray 80% ethanol on the inside of the tray and the incision of the coconut inflorescence and ignite it. The spray volume of ethanol is 22ml. After the flame is extinguished, spray 80% ethanol and ignite again. Repeat this 4 times ;Such as Figure 1~4 Shown

[0054] (3) When lighting for the 4th time, put the coconut inflorescence together with the tray into the ultra-c...

Example Embodiment

[0056] Example 3

[0057] A method for sterilizing coconut inflorescence, including the following steps:

[0058] A. Pre-sterilization:

[0059] Select the unopened coconut inflorescences with buds, wash them with 70% ethanol for 2 minutes, soak them in an antioxidant solution for 25 minutes, and wash them again with 70% ethanol to remove the residual antioxidant solution. The bacteria are blown dry; among them, the antioxidant solution is a mixture of 1g / L citric acid and 1g / L ascorbic acid;

[0060] B. Dry and sterilize:

[0061] (1) Put the pre-sterilized coconut inflorescence in the tray;

[0062] (2) Evenly spray 70% ethanol on the inside of the tray and the incision of the coconut inflorescence and ignite it. The spray volume of ethanol is 18ml. After the flame is extinguished, spray 70% ethanol and ignite again, repeat 4 times ; The burning time of each flame is> 2min;

[0063] (3) When lighting for the 4th time, put the coconut inflorescence together with the tray into the ultra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a sterilization method of coconut inflorescences. The sterilization method comprises the steps that non-blossom coconut inflorescences with spathes are selected, ethyl alcohol with the mass concentration of 70-80% is uniformly sprayed to cuts of the coconut inflorescences at least four times and ignited, and then spicae inside the coconut inflorescences are sterilized. By means of the sterilization method of the coconut inflorescences, the environmental pollution can be reduced, the cost of reagents is reduced, spica explants are prevented from getting injured, the browning phenomenon does not exist, the test procedure is simplified, the test time is shortened, and the safety and operation convenience of coconut tissue culture are improved.

Description

technical field [0001] The invention relates to the technical field of coconut tissue culture, in particular to a method for sterilizing coconut inflorescences. Background technique [0002] In plant tissue culture, the disinfection and sterilization of explant material affects the subsequent tissue culture effect. Coconut inflorescence is an important explant material in coconut tissue culture, and the inflorescence is relatively easy to obtain, and there are many reports on the successful induction of regenerated plants. [0003] But the sterilization method of existing coconut inflorescence explant all adopts the mode of chemical reagent sterilization, generally is chemical reagents such as mercury chloride, sodium hypochlorite soaking sterilization, and there are many problems in chemical reagent sterilization. On the one hand, chemical reagents will cause environmental pollution, material pollution, damage to explants, browning of explants, death of explants, and incre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 谢里夫·法西·艾德·赛义德·朱斯范海阔
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap