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Sterilization method of coconut inflorescences

A sterilization method and inflorescence technology, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of increased test costs, cumbersome processing steps, and damage to explants, and achieve good disinfection and sterilization effects. Sterilization efficiency and damage reduction effect

Inactive Publication Date: 2019-07-09
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, chemical reagents will cause environmental pollution, material pollution, explant damage, explant browning, explant death, and increased test costs
On the other hand, in the test process, it is often necessary to soak the explants with chemical reagents for many times, and then wash them with sterile water for many times. Finally, it takes a long time to wait for the materials to dry, etc. There are disadvantages such as cumbersome processing steps and long processing time

Method used

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  • Sterilization method of coconut inflorescences
  • Sterilization method of coconut inflorescences
  • Sterilization method of coconut inflorescences

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for sterilizing coconut inflorescences, comprising the steps of: selecting unopened coconut inflorescences with spathes, putting them into a tray, evenly spraying mass concentration of 70% ethanol at the incision of coconut inflorescences and igniting them. After the flame was extinguished, evenly spray the mass concentration of 70% ethanol at the incision of the coconut inflorescence again and ignite it, repeat this 4 times, the spraying amount of ethanol is 18ml each time, sterilize the flower spike inside the coconut inflorescence, and wait for the flame to go out Finally, the spathe was cut open, and the inner flower spike material was taken out for the inoculation test.

Embodiment 2

[0048] A kind of sterilization method of coconut inflorescence, comprises the steps:

[0049] A. Pre-sterilization:

[0050] Select unopened coconut inflorescences with spathes, wash them with 73% ethanol for 1.5 minutes, soak them in the antioxidant solution for 20 minutes, wash them again with 73% ethanol to remove the residual antioxidant solution, and use Drying with sterile wind; wherein, the antioxidant solution is mixed with 1g / L citric acid and 1g / L ascorbic acid by mass concentration;

[0051] B. Dry sterilization:

[0052] (1) put the coconut inflorescence through pre-sterilization in the tray;

[0053] (2) Evenly spray mass concentration to the inside of the tray and the kerf of coconut inflorescences and ignite it with 80% ethanol. The amount of ethanol sprayed is 22ml. After the flame is extinguished, spray mass concentration again with 80% ethanol and ignite. Repeat 4 times like this ;like Figure 1~4 shown;

[0054] (3) When lighting for the 4th time, put t...

Embodiment 3

[0057] A kind of sterilization method of coconut inflorescence, comprises the steps:

[0058] A. Pre-sterilization:

[0059] Select unopened coconut inflorescences with spathes, wash them with 70% ethanol for 2 minutes, soak them in the antioxidant solution for 25 minutes, wash them again with 70% ethanol to remove the residual antioxidant solution, and use no Drying with bacterial wind; wherein, the antioxidant solution is mixed with 1g / L citric acid and 1g / L ascorbic acid at a mass concentration;

[0060] B. Dry sterilization:

[0061] (1) put the coconut inflorescence through pre-sterilization in the tray;

[0062] (2) Evenly spray mass concentration to the inside of the tray and the kerf of coconut inflorescences and ignite it with 70% ethanol. The amount of ethanol sprayed is 18ml. After the flame is extinguished, spray mass concentration again with 70% ethanol and ignite, repeat 4 times like this ;The burning time of each flame is >2min;

[0063] (3) When lighting fo...

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Abstract

The invention provides a sterilization method of coconut inflorescences. The sterilization method comprises the steps that non-blossom coconut inflorescences with spathes are selected, ethyl alcohol with the mass concentration of 70-80% is uniformly sprayed to cuts of the coconut inflorescences at least four times and ignited, and then spicae inside the coconut inflorescences are sterilized. By means of the sterilization method of the coconut inflorescences, the environmental pollution can be reduced, the cost of reagents is reduced, spica explants are prevented from getting injured, the browning phenomenon does not exist, the test procedure is simplified, the test time is shortened, and the safety and operation convenience of coconut tissue culture are improved.

Description

technical field [0001] The invention relates to the technical field of coconut tissue culture, in particular to a method for sterilizing coconut inflorescences. Background technique [0002] In plant tissue culture, the disinfection and sterilization of explant material affects the subsequent tissue culture effect. Coconut inflorescence is an important explant material in coconut tissue culture, and the inflorescence is relatively easy to obtain, and there are many reports on the successful induction of regenerated plants. [0003] But the sterilization method of existing coconut inflorescence explant all adopts the mode of chemical reagent sterilization, generally is chemical reagents such as mercury chloride, sodium hypochlorite soaking sterilization, and there are many problems in chemical reagent sterilization. On the one hand, chemical reagents will cause environmental pollution, material pollution, damage to explants, browning of explants, death of explants, and incre...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 谢里夫·法西·艾德·赛义德·朱斯范海阔
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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