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Phoma herbarum water dispersible granules and preparing and using method thereof

A technology of Phytophthora sp. and dispersing granules, which is applied in the fields of botanical equipment and methods, herbicides and algicides, and applications, can solve problems such as loss of activity, inability to guarantee growth conditions, and reduce control effects, and achieve cost savings. , Not easy to exceed the standard dust, good suspension effect

Inactive Publication Date: 2019-07-12
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the mycelium is not processed into preparations, the necessary growth conditions cannot be guaranteed, causing it to lose its activity and reduce the control effect. At the same time, the maximum control effect can be exerted only by relying on the carrier of the preparation. Really put into production and practical application
At present, there is no relevant report about Phoma herbaceous being processed into water-dispersible granules

Method used

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  • Phoma herbarum water dispersible granules and preparing and using method thereof
  • Phoma herbarum water dispersible granules and preparing and using method thereof
  • Phoma herbarum water dispersible granules and preparing and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0042] Example 1: Determination of biocompatibility of adjuvants with Phomophora phlox

[0043] experiment material:

[0044] Carriers include diatomaceous earth, attapulgite, quartz sand, bentonite, talc, and white carbon black;

[0045] Wetting agents include sodium dodecylbenzenesulfonate, PE-100, AK-311, pull-off powder, sodium dodecyl sulfate;

[0046] Dispersants include sodium lignosulfonate, MF, erucamide, NNO, oleamide;

[0047] Binders include gelatin, gum arabic, polyvinyl alcohol, MF, erucamide;

[0048] Disintegrants include urea, soluble starch, sodium sulfate, sodium chloride, ammonium sulfate;

[0049] Stabilizers include calcium carbonate, dipotassium phosphate, borax, oxalic acid, potassium tripolyphosphate.

[0050] experiment method:

[0051] The above-mentioned dispersant, wetting agent, binder, disintegrant and stabilizer were respectively prepared into suspensions with a concentration of 1000 μg / mL, and 2 mL of the suspension was mixed with molten P...

Embodiment 2

[0065] Embodiment 2: the preparation method of Phoma phlox water-dispersible granule

[0066] A kind of Phylomyces phlox water-dispersible granules, wherein the raw material components of Phymophthora phlegm water-dispersible granules include Phymophthora phlegm 15, wetting agent 6, dispersant 4, disintegrating agent 6, binder 6, stable Agent 5, carrier 58.

[0067] Its preparation method is as follows:

[0068] Preparation of Phomomyces phlox mycelia: Inject Phomomyces phlox into PD medium (200g of potatoes, 20g of glucose, 1000ml of distilled water), 25-28°C, 170-200r / min, shake culture for 7-10 days, collect The mycelium is ground and pulverized by an ultrasonic oscillator to obtain mycelium fragments.

[0069] Weigh 6g of pulling powder, 4g of NNO, 6g of ammonium sulfate, 6g of polyethylene glycol, 5g of calcium carbonate, and add the bacteria content to be 3×10 8 Cfu / g mycelia 15g, white carbon black 58g. Refer to GB / T1601-93 Pesticide pH Determination Method, and adj...

Embodiment 3

[0071] Embodiment 3: The control test of Phoma phlox water-dispersible granule to Commelina chinensis

[0072] Experimental method 1: Spray active ingredients at the 1~3 leaf stage, 3~4 leaf stage, and 5~6 leaf stage of Commelina japonicus with 2.55×10 8 cfu / g Phytophthora water dispersible granules, with 25% bentazone water as the control agent, with the treatment of spraying clear water as the blank control, 4 repetitions are set for each treatment, and 6 pots of seedlings are used for each repetition . Put them in a climate incubator and cultivate them under the conditions of temperature 20-30°C, humidity 60%-80%RH, light intensity about 1000LX, and light time 10h.

[0073] Experimental method 2: At the 3rd to 4th leaf stage of commelina, the active ingredient was sprayed at 2.55×10 8 Cfu / g Phytophthora water-dispersible granules, spray once every 7 days, spray 3 times altogether, take the treatment of spraying clear water as blank control, set 4 repetitions for each trea...

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Abstract

The invention relates to phoma herbarum water dispersible granules and a preparing and using method thereof. The phoma herbarum water dispersible granules are prepared from 15 parts of phoma herbarum,2-8 parts of a wetting agent, 2-6 parts of a dispersing agent, 2-8 parts of a disintegrating agent, 4-6 parts of an adhesive, 5-7 parts of a stabilizer and 50-70 parts of a carrier. The preparing method of the phoma herbarum water dispersible granules comprises the steps of preparation of phoma herbarum hyphae, mixing, kneading, pelleting and drying. Mist spraying treatment is conducted in the 3-4-leaf stage of commelina communis L and the 3-4-leaf stage of amaranthus retroflexus, the pesticide application amount is 228-236 g / mu, mist spraying is conducted once every 7 days, and mist sprayingis totally conducted 3 times. By means of the preparing and using method, the biocontrol bacterium phoma herbarum is processed into the water dispersible granules, the biocontrol activity of the phoma herbarum is guaranteed, the granules are safe to operators and environments, the concentration of effective components is high, the storage stability is high, and the shelf life is long.

Description

technical field [0001] The invention relates to biological pesticides, in particular to a Phoma phlox water-dispersible granule and a preparation and use method thereof. Background technique [0002] Commelina ( Commelina communis L ) is a vicious weed in the field, with rapid growth, strong damage potential, and strong adaptability in the field. Artificial weeding is not effective, and chemical weeding has problems such as pesticide residues, environmental pollution, and drug resistance. The use of biological control of weeds can provide a new way for weed control. [0003] Phoma ( Phoma herbarum ) is a pathogenic fungus isolated from the diseased Commelina miltiorrhiza. Phomophora phlox can infect Commelina praecox and cause its death. When the fungal herbicide conditions are suitable, it can be applied in large doses in the field to artificially create target weeds. The disease pandemic, so as to quickly and effectively control weeds. Fungal herbicides have the chara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/14A01P13/00
CPCA01N25/14A01N63/30
Inventor 谷祖敏纪明山邹益泽王英姿祁之秋何璐
Owner SHENYANG AGRI UNIV
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