Whole-body high-resolution multispectral optical imaging system for living body of small animal

Abstract

The invention discloses a whole-body high-resolution multispectral optical imaging system for a living body of a small animal. The system comprises an excitation light source, an excitation light spectroscope cone, an emitting light filter set, a microscope cone, an emitting light spectroscope cone, an imaging detector, a three-dimensional motion scanning platform, a rack and an image collecting workstation. The excitation light source enters the excitation light spectroscope cone through optical fiber coupling to form coaxial excitation light, the living body sample of the small animal on theplatform generates emitting light under irradiation of the excitation light, and the emitting light is filtered by the emitting light filter set; the microscope cone and the emitting light spectroscope cone conduct imaging at a visible light CCD and a near-infrared CCD respectively, the image collecting workstation connected with the detector can control the three-dimensional motion scanning platform to conduct high-resolution optical focusing and XY-direction whole-body scanning imaging in real time, and thus whole-body high-resolution multispectral optical imaging of the living body of thesmall animal is achieved. By means of the system, ultrahigh-optical-resolution imaging of visible light and near-infrared light of various wavebands can be achieved simultaneously, and the whole bodyof the living body of the small animal can be subjected to scanning imaging; the system is simple in structure and convenient to operate.

Description

technical field [0001] The invention relates to the field of small animal living optical imaging systems, in particular to a small animal living whole body high-resolution multispectral optical imaging system. Background technique [0002] At present, the in vivo optical imaging system for small animals is mainly an in vivo imaging device that uses fluorescence or bioluminescence to achieve specific tracking of molecular targets. ), the highest resolution of local imaging has reached the um level, with good time resolution and high spatial resolution, and is regarded as the most promising next-generation optical imaging technology. However, at present, there is no integrated equipment and system that can realize high-resolution multispectral optical imaging of the whole body of small animals at the same time. Contents of the invention [0003] In order to overcome the deficiencies in the prior art, the present invention provides a whole-body high-resolution multispectral ...

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Problems solved by technology

However, at present, there is no integrated equipment and system that can realize high-res

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