Identification system and method of SCD plasmodiophora physiological race
A technology of physiological races and Plasmodium, applied in the direction of microorganism-based methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problem of affecting the breeding of disease-resistant varieties and the accurate identification of Plasmodium physiological races Sex and other issues, to achieve the effect of simple and easy to implement identification method, and speed up the breeding process
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[0035] Example 1 An SCD (Sinitic Clubroot Differentiation system) physiological race identification system of rhizobia (hereinafter referred to as SCD identification system), as shown in Table 1, P1-P14 in Table 1 are physiological race types.
[0036] Table 1 Physiological race identification system of SCD rhizobia
[0037]
[0038] Example 2 Ways to obtain and verify the SCD identification system of Example 1
[0039] Step 1. Collect host materials
[0040] Hosts of 12 Chinese cabbage inbred lines (code H1-H12): including 11 root-swelling resistant lines and 1 susceptible line used in this study. For host information, see Table 2. Chinese cabbage inbred lines (H1-H11) against root swelling disease were used for host screening in a stable and accurate physiological race classification system, and susceptible line H12 was used as a positive control for root swelling disease resistance test. The hosts of Williams identification system: Badger shipper, Jersey Queen, Laurentian and Wilh...
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[0075] Example 3 Comparison and advantages of the SCD identification system of the present invention and the prior art
[0076] (1) A brief description of existing foreign identification systems: The economic losses caused by cruciferous crop root swelling are well known. Accurate identification of the physiological races of rhizobia is one of the prerequisites for the selection of disease-resistant varieties. Scientists from all over the world have made great efforts on the development of the physiological race differentiation system of Brassica rhizobia and achieved remarkable results. However, the previously developed Williams identification system, ECD system and the identification system developed by Somé et al. cannot distinguish some physiological races in Canada, Korea, Japan and China (References (4)-(6), Kuginuki et al. 1999; Kim et al. 2016; Strelkov et al. 2018). The host used in the Williams identification system and the identification system of Somé et al. (Refere...
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