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Identification system and method of SCD plasmodiophora physiological race

A technology of physiological races and Plasmodium, applied in the direction of microorganism-based methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problem of affecting the breeding of disease-resistant varieties and the accurate identification of Plasmodium physiological races Sex and other issues, to achieve the effect of simple and easy to implement identification method, and speed up the breeding process

Pending Publication Date: 2019-07-12
SHENYANG AGRI UNIV
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AI Technical Summary

Problems solved by technology

This will seriously affect the accuracy of identification of Physiological races of Plasmodium, thereby affecting the breeding of disease-resistant varieties

Method used

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  • Identification system and method of SCD plasmodiophora physiological race
  • Identification system and method of SCD plasmodiophora physiological race
  • Identification system and method of SCD plasmodiophora physiological race

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 An SCD (Sinitic Clubroot Differentiation system) Phytophthora physiological race identification system (hereinafter referred to as the SCD identification system), as shown in Table 1, P1-P14 in Table 1 is the physiological race type.

[0036] Table 1 Identification system of physiological races of SCD Plasmodium

[0037]

[0038] Embodiment 2 The acquisition route and verification of the SCD identification system of Embodiment 1

[0039] Step 1. Collect host material

[0040] Hosts of 12 Chinese cabbage inbred lines (codes H1-H12): including 11 clubroot-resistant lines and 1 susceptible line used in this study. See Table 2 for host information. The clubroot-resistant Chinese cabbage inbred lines (H1-H11) were used for stable and accurate physiological race classification system host selection, and the susceptible line H12 was used as a positive control for clubroot resistance tests. The hosts of the Williams differential system: Badger shipper, Jersey Que...

Embodiment 3

[0075]Embodiment 3 Comparison and advantages of the SCD identification system of the present invention and the prior art

[0076] (1) Brief description of existing foreign identification systems: the economic losses caused by clubroot of cruciferous crops are well known. Accurate identification of the physiological race of Plasmodium is one of the prerequisites for the selection of disease-resistant varieties. Scientists from all over the world have made great efforts on the development of the race differentiation system of Plasmodium brassica and achieved remarkable results. However, the previously developed Williams differential system, the ECD system, and the differential system developed by Somé et al. were unable to distinguish some races in Canada, Korea, Japan, and China (References (4)–(6), Kuginuki et al. 1999; Kim et al. 2016; Strelkov et al. 2018). The hosts used in the Williams identification system and the identification system of Somé et al. (ref. (3)) did not ...

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Abstract

The invention belongs to the technical field of identification of a plasmodiophora brassica physiological race, and particularly relates to an identification system and method of an SCD plasmodiophoraphysiological race. The identification system of the SCD plasmodiophora physiological race comprises 9 host materials and 14 physiological race types. To-be-detected plasmodiophora is inoculated intothe 9 host materials, and the type of the physiological race of the to-be-detected plasmodiophora can be judged according to the disease resistance of the host materials. According to the SCD identification system and the corresponding identification method, classification, distribution, variation, prevalence and prevention research of clubroot physiological races are facilitated, and the breeding process of anti-clubroot Chinese cabbage and other brassica crops in China is greatly accelerated.

Description

technical field [0001] The invention belongs to the technical field of identification of physiological races of Plasmodium genus Brassica, and specifically relates to an identification system, method and application of physiological races of SCD Plasmodium. Background technique [0002] The rapid spread of clubroot caused by the soil-borne obligate parasitic plant pathogen Plasmodium has become one of the most serious diseases of cruciferous crops worldwide. Plasmodium infection leads to host root cell proliferation and hypertrophy, which inhibit nutrient and water transport leading to plant wilting and reduced plant yield. Clubroot can affect nearly all Brassica species, including Chinese cabbage, cabbage, canola, and radishes. Crop rotation and chemical treatments such as fluazinam and cyazofamid have been shown to be effective against clubroot. However, crop rotation will take a long time because Plasmodium can survive in soil for more than 15 years; chemical treatment ...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12Q1/04C12R1/645
CPCC12Q1/18G01N2333/37Y02A50/30
Inventor 朴钟云庞文星战宗祥李晓楠梁月
Owner SHENYANG AGRI UNIV
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