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Medium for prolonging life of fruit flies and preparation and use methods thereof

A culture medium, Drosophila technology, applied in the application, resistance to vector-borne diseases, animal feed, etc., can solve the problems of large amount of addition, difficult separation and purification, limited application prospects, etc., to achieve the effect of prolonging lifespan

Active Publication Date: 2019-07-16
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] People such as Zheng Yuan of Anhui University (Chinese Edible Fungi, 2006, 25 (3): 38-41, the research of anti-aging effect of polysaccharide in Phellinus Phellinus) adopt Phellinus Phellinus intracellular polysaccharide to play as Drosophila culture medium additive and have played prolongation Drosophila. The role of lifespan, but the pure Phellinus polysaccharides are difficult to separate and purify; and the addition amount of Phellinus intracellular polysaccharides in this experiment needs to be 4.0% of the weight of the culture medium, the addition amount is too large, the cost remains high, and the practical application prospect limited

Method used

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  • Medium for prolonging life of fruit flies and preparation and use methods thereof
  • Medium for prolonging life of fruit flies and preparation and use methods thereof
  • Medium for prolonging life of fruit flies and preparation and use methods thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] see figure 1 , a preparation method of a medium for prolonging the lifespan of fruit flies, comprising the steps of:

[0036] (1) Obtaining the fruiting bodies of Phellinus sanghuang: The fruiting bodies of Phellinus sangellia were collected from Jilin, and were identified as Phellinus sphaerifolia by experts in the classification of edible fungi.

[0037] (2) Preparation of Phellinus Phellinus water body fluid: boil the dried Phellinus Phellinus fruiting body small particles and water in a ratio of 1:10 in boiling water at 100°C for 2 hours, centrifuge at room temperature for 30 minutes at 12,000 rpm, and filter the residue for secondary extraction , combined and collected the supernatant into a 50mL sterilized tube;

[0038] (3) Place the centrifuge tube containing the supernatant in a freeze dryer to freeze-dry it into a dry powder for later use; the freezing temperature of the freeze dryer is -55°C, and the drying time is 15 days;

[0039] (4) First configure the ...

Embodiment 2

[0051] Adopt the method for embodiment 1, the application in Drosophila melanogaster cultivation, specifically as follows:

[0052] (1) Drosophila: Drosophila melanogaster;

[0053] (2) Treatment method: starve the fruit flies for 2-4 hours first, anesthetize the newly eclovened fruit flies with carbon dioxide, pick out 10 male and female fruit flies, and place the bottle on its side to prevent the fruit flies from sticking to the bottle. On the culture medium, after the fruit flies wake up, the culture bottle can be placed in a constant temperature incubator for cultivation, and each treatment is repeated 3 times:

[0054] (3) Culture method: Put the control group and the Phellinus japonica water extract group into the same culture, set the temperature at 25±1°C, and the relative humidity is about 60%, light:dark=12h:12h; add Phellinus japonica water The concentration used in the extract group was 1 μg / ml.

[0055] (4) Observing the control group and the treatment group: ob...

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Abstract

The invention relates to the technical field of biological mediums, in particular to a medium for prolonging the life of fruit flies and preparation and use methods thereof. The preparation method includes the following steps: (1) obtaining a Phellinus igniarius fruit body; (2) preparing a Phellinus igniarius water extract; (3) conducting freeze drying; (4) formulating a mother liquor; (5) conducting diluting; and (6) formulating a medium. The medium containing the Phellinus igniarius water extract is added, and by observing the life of fruit flies, the medium can significantly prolong the life of fruit flies. Compared with a control group, under same feeding conditions including temperature, humidity and light conditions, through calculation on the semi-lethal dose, the added Phellinus igniarius water extract can significantly increase the semi-lethal days by 7d compared with a control medium, thereby significantly prolonging the lifespan of fruit flies.

Description

technical field [0001] The invention relates to the technical field of biological culture medium, in particular to a fruit fly culture medium. Background technique [0002] As an important model organism, Drosophila has the characteristics of strong fecundity, short generation cycle, many types of variation, and simple breeding. At present, it has been widely used in various research fields of life science. In particular, about 75% of the genes and metabolic pathways of Drosophila homologous to human diseases are very similar to humans, and they are used as model organisms for the study of aging and aging-related diseases. [0003] In recent years, the scientific research team using Drosophila as a model organism has continued to grow. Drosophila traits are affected by the interaction of environment and genetic Drosophila medium components. , reproduction and survival have an important impact. Therefore, the optimization of Drosophila medium components has become a resear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/90A23K10/30A23K10/18A01K67/033
CPCA23K50/90A23K10/30A23K10/18A01K67/033Y02A50/30
Inventor 侯立娟蒋宁马林曲绍轩李辉平林金盛
Owner JIANGSU ACAD OF AGRI SCI
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