Combined repair method for copper-polluted soil
A joint restoration and copper-polluted technology, applied in the field of joint restoration of copper-contaminated soil, can solve the problems of difficult removal of heavy metals and increase of resource input, and achieve the effect of saving resources, simple operation, saving time and resources
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Embodiment 1
[0032] Specifically include the following steps:
[0033] Step 1: Add the microbial agent to the copper-contaminated soil at an application rate of 35kg / mu, and turn it evenly with mechanical equipment such as a tiller, so that the microbial agent is evenly distributed in the contaminated area;
[0034] The specific steps for the preparation of the microbial inoculum are:
[0035] 1) Slant culture: Pick 1 loop of the original strains of Penicillium oxalicum and C. chrysogenum under aseptic conditions and inoculate them on the slant medium respectively, and cultivate them at 28°C for 72 hours; among them, the slant medium formula For: glucose 20g / L, potato juice 200g / L, agar 20g / L, pH natural;
[0036] 2) Shaker culture: add sterile water to scrape the spores on the slant of the strains cultured in step 1), and dilute to a spore content of 1×10 7 The spore suspension of cfu / mL was respectively inoculated in the seed medium under sterile conditions, and cultured on a shaker at...
Embodiment 2
[0044] Specifically include the following steps:
[0045] Step 1: Add the microbial agent to the copper-contaminated soil at an application rate of 35kg / mu, and turn it evenly with mechanical equipment such as a tiller, so that the microbial agent is evenly distributed in the contaminated area;
[0046] The specific steps for the preparation of the microbial inoculum are:
[0047]1) Slant culture: Pick 1 loop of the original strains of Penicillium oxalicum and C. chrysogenum under aseptic conditions and inoculate them on the slant medium respectively, and cultivate them at 26°C for 48 hours; among them, the slant medium formula For: glucose 20g / L, potato juice 200g / L, agar 20g / L, pH natural;
[0048] 2) Cultivation on a shaker: Add sterile water to the slant of the strain cultured in step 1) to scrape the spores and dilute them to a spore content of 1*10 6 The spore suspension of cfu / mL was respectively inoculated in the seed medium under aseptic conditions, and cultured on ...
Embodiment 3
[0055] Specifically include the following steps:
[0056] Step 1: Add the microbial agent to the copper-contaminated soil at an application rate of 30kg / mu, and use mechanical equipment such as a tiller to turn it evenly, so that the microbial agent is evenly distributed in the contaminated area;
[0057] All the other steps are the same as in Example 1.
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