Combined repair method for copper-polluted soil

A joint restoration and copper-polluted technology, applied in the field of joint restoration of copper-contaminated soil, can solve the problems of difficult removal of heavy metals and increase of resource input, and achieve the effect of saving resources, simple operation, saving time and resources

Active Publication Date: 2019-07-16
湖南重金属污染耕地安全高效利用工程研究中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Appropriate use of phytoremediation of heavy metal-contaminated soil can effectively remove heavy metals or reduce their biological toxicity, but for areas with high pollution levels, i

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Specifically include the following steps:

[0033] Step 1: Add the microbial agent to the copper-contaminated soil at an application rate of 35kg / mu, and turn it evenly with mechanical equipment such as a tiller, so that the microbial agent is evenly distributed in the contaminated area;

[0034] The specific steps for the preparation of the microbial inoculum are:

[0035] 1) Slant culture: Pick 1 loop of the original strains of Penicillium oxalicum and C. chrysogenum under aseptic conditions and inoculate them on the slant medium respectively, and cultivate them at 28°C for 72 hours; among them, the slant medium formula For: glucose 20g / L, potato juice 200g / L, agar 20g / L, pH natural;

[0036] 2) Shaker culture: add sterile water to scrape the spores on the slant of the strains cultured in step 1), and dilute to a spore content of 1×10 7 The spore suspension of cfu / mL was respectively inoculated in the seed medium under sterile conditions, and cultured on a shaker at...

Embodiment 2

[0044] Specifically include the following steps:

[0045] Step 1: Add the microbial agent to the copper-contaminated soil at an application rate of 35kg / mu, and turn it evenly with mechanical equipment such as a tiller, so that the microbial agent is evenly distributed in the contaminated area;

[0046] The specific steps for the preparation of the microbial inoculum are:

[0047]1) Slant culture: Pick 1 loop of the original strains of Penicillium oxalicum and C. chrysogenum under aseptic conditions and inoculate them on the slant medium respectively, and cultivate them at 26°C for 48 hours; among them, the slant medium formula For: glucose 20g / L, potato juice 200g / L, agar 20g / L, pH natural;

[0048] 2) Cultivation on a shaker: Add sterile water to the slant of the strain cultured in step 1) to scrape the spores and dilute them to a spore content of 1*10 6 The spore suspension of cfu / mL was respectively inoculated in the seed medium under aseptic conditions, and cultured on ...

Embodiment 3

[0055] Specifically include the following steps:

[0056] Step 1: Add the microbial agent to the copper-contaminated soil at an application rate of 30kg / mu, and use mechanical equipment such as a tiller to turn it evenly, so that the microbial agent is evenly distributed in the contaminated area;

[0057] All the other steps are the same as in Example 1.

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PUM

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Abstract

The invention provides a combined repair method for copper-polluted soil. The method comprises the following steps: adding a microbial agent into copper-polluted soil according to an application amount of 25-45 kg/mu, carrying out overturning, and then planting equisetum ramosissimum in the copper-polluted soil containing the microbial agent, so that copper in the soil is removed by combining absorption of the equisetum ramosissimum on copper and an reinforcing effect of the microbial agent, wherein the microbial agent is mixed spore powder of penicillium oxalicum and tarlaromyces flavus. According to the provided combined repair method for the copper contaminated soil, through activation effect of microorganisms, copper with an insoluble state or a weak acid salt binding state in the contaminated soil is converted into exchangeable copper ions which can be easily absorbed by plants. Heavy metals are enriched in the plants through growth and propagation of the plants, and then the heavy metals are removed. The operation mode is simple and is low in cost, the heavy metal content in the copper-polluted soil can be remarkably reduced, and the method can be used for repairing copper-polluted soil areas with different pollution degrees.

Description

technical field [0001] The invention relates to the field of contaminated soil treatment, in particular to a combined restoration method for copper-contaminated soil. Background technique [0002] Soil is not only an important part of the earth's ecological circle, but also an important natural resource for human survival. With the development of industrial and agricultural production, soil heavy metal pollution has become more and more serious, and soil pollution has become one of the most concerned public hazards. Accumulated over the years, the problems of land reclamation and ecological restoration faced by people have become very serious. The traditional remediation methods of copper pollution in soil are usually physical and chemical methods, such as dilution and soil covering method, vitrification technology, heat treatment technology, leaching method, electrochemical method, etc. Although the traditional remediation methods have obvious treatment effects, they laste...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/10B09C1/105
Inventor 郭帅张冬雪文亚雄邱尧
Owner 湖南重金属污染耕地安全高效利用工程研究中心有限公司
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