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A kind of mandibular condyle osteochondral repair scaffold material and preparation method thereof

A scaffold material and osteochondral technology, applied in the direction of bone/connective tissue cells, prostheses, tissue regeneration, etc., can solve problems such as repair and inability to realize osteochondral defect of mandibular condyle, and achieve the effect of promoting adhesion

Active Publication Date: 2022-02-22
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the fact that in the prior art there is no scaffold material specifically for osteochondral repair of the mandibular condyle, the clinically applied methods and materials for the treatment of mandibular condyle osteochondral injury cannot repair the mandibular condyle osteochondral defect, The invention provides a mandibular condylar osteochondral repair scaffold material and a preparation method thereof, so as to realize the repair of the mandibular condylar osteochondral defect

Method used

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  • A kind of mandibular condyle osteochondral repair scaffold material and preparation method thereof
  • A kind of mandibular condyle osteochondral repair scaffold material and preparation method thereof
  • A kind of mandibular condyle osteochondral repair scaffold material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] In this embodiment, mercapto-hyaluronic acid (HA-SH) is prepared, and the steps are as follows:

[0041] (1) Dissolve 2 g of sodium hyaluronate (HA) with a molecular weight of 0.3 MDa in deionized water, add 10 mmol of N-hydroxysuccinimide (NHS), stir in deionized water at room temperature until completely dissolved, and then Add 25 mmol of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), fully dissolve, and adjust the mixing with 1 mol / L NaOH solution and 1 mol / L HCl solution The pH value of the solution was 4.75-5.0, reacted at room temperature for 2 hours, then added 25 mmol of cysteine ​​hydrochloride (CSH·HCl), reacted at room temperature for 24 hours, and then transferred the solution to a dialysis bag with a molecular weight cut-off of 8000-14000 KDa, and kept at room temperature Dialysis was performed for 72 hours, and finally the dialysate was freeze-dried to obtain solid HA-SH.

[0042] HA and HA-SH are detected by nuclear magnetic resona...

Embodiment 2

[0046] In this example, the scaffold material for osteochondral repair of the mandibular condyle was prepared, and the steps were as follows:

[0047] (1) Place the formed porous biphasic calcium phosphate ceramics (porous BCP ceramics) in a cylindrical mold with an inner diameter of 8 mm and a height of 3 mm. The porous BCP ceramics are composed of hydroxyapatite and β-tricalcium phosphate, and the hydroxyl The mass ratio of apatite to β-tricalcium phosphate is 3:7, and the porosity is about 75%. The formed porous BCP ceramics is a cylindrical block with a diameter of 8mm and a height of 1.5mm. The formed porous BCP ceramics adopts the same mold The coaxial way is placed on the closed end of the cylindrical mold.

[0048] (2) Obtain primary cultured bone marrow mesenchymal stem cells and chondrocytes: select 5-day-old infant rabbits, inject pentobarbital sodium to kill them, and extract bone marrow mesenchymal stem cells from bilateral femoral bone marrow cavities by centrifu...

Embodiment 3

[0064] In this example, the physical and chemical properties of the composite hydrogel (HA-SH / Col I) of cross-linked mercapto-hyaluronic acid and type I collagen (HA-SH / Col I) were tested. as a control for comparison.

[0065] Dissolve the HA-SH prepared in Example 1 with α-MEM medium to obtain a concentration of HA-SH solution of 25 mg / mL; dissolve Col I with 0.25 mol / L acetic acid solution in an ice bath to obtain a concentration of 25 mg / mL Col I solution. Draw the HA-SH solution and Col I solution with a syringe respectively, and mix the HA-SH solution and Col I solution according to the volume ratio of 3:7 according to the group, adjust the pH to 7.4 with NaOH solution, and let it stand for a while at 37°C. Composite hydrogel HA-SH / Col I can be formed. Dissolve the HA-SH prepared in Example 1 with α-MEM medium to obtain a HA-SH solution with a concentration of 25 mg / mL, adjust the pH to 7.4 with NaOH solution, and stand at 37°C for a period of time to form HA-SH water ...

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Abstract

The invention provides a scaffold material for mandibular condylar osteochondral repair and a preparation method thereof. The scaffold material is composed of a cartilage surface layer, a cartilage deep layer and a bone layer, and the cartilage surface layer is composed of cross-linked mercapto-hyaluronic acid and type I collagen. Composed of hydrogel and bone marrow mesenchymal stem cells distributed in the composite hydrogel, the deep layer of cartilage is composed of a composite hydrogel of cross-linked mercapto-hyaluronic acid and type I collagen and cartilage distributed in the composite hydrogel Composed of cells, the bone layer is porous biphasic calcium phosphate ceramics, the cartilage deep layer is located between the cartilage surface layer and the bone layer, and the porous structure of the bone layer connects the cartilage surface layer and the bone layer as a whole. In vivo experiments have confirmed that the scaffold material has excellent repair ability for mandibular condylar osteochondral defects, and can solve the problem that the existing methods and materials for the treatment of mandibular condylar osteochondral injuries cannot repair mandibular condylar osteochondral defects. The treatment of mandibular condylar osteochondral injuries offers new possibilities.

Description

technical field [0001] The invention belongs to the field of mandibular bone repair materials, and relates to a mandibular condyle cartilage repair support material and a preparation method thereof. Background technique [0002] Temporomandibular joint disorder is very common clinically, often accompanied by osteochondral defects of the mandibular condyle, leading to joint pain, limited mandibular movement, osteoarthritis, and even limited mouth opening, seriously affecting the quality of life of patients . The mandibular condyle lacks direct blood supply and innervation, and its self-repair ability is extremely poor. The osteochondral defect of the condyle caused by temporomandibular joint disorder can hardly be completely repaired. At present, there are obvious shortcomings in the clinically applied methods for the treatment of mandibular condylar osteochondral injury: (1) non-invasive treatments such as oral medicine and physical therapy can only relieve symptoms and del...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077A61L27/40A61L27/44A61L27/42A61L27/38A61L27/52A61L27/56C08J3/24C08J3/075C08L5/08
CPCA61L27/44A61L27/425A61L27/3817A61L27/3834A61L27/56A61L27/52C08J3/24C08J3/075A61L27/3886C08J2305/08A61L2430/06
Inventor 孙勇王鸿哲徐扬王培磊樊渝江
Owner SICHUAN UNIV
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