A method for joint restoration of heavy metal-contaminated soil by using Trichoderma viridans and Suaeda salsa
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A technology of combined restoration of polluted soil, applied in the field of soil restoration, can solve the problems of small biomass, less total absorption and accumulation, and slow growth
Active Publication Date: 2020-04-17
QINGDAO AGRI UNIV
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Like other heavy metal-enriched plants, Suaeda salsa also has bottleneck problems such as small biomass, slow growth, and low absorption and accumulation of heavy metals by plants per unit area.
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Embodiment 1
[0045] Soil for test: farmland soil in a town of Pingdu City, its basic physical and chemical properties are: pH 6.62 (soil-water ratio 1:2.5), organic matter 16.6g / kg, cation exchange capacity 19.8cmol / kg, total copper content 53.5mg / kg kg, the total cadmium content is 1.08mg / kg, the total zinc content is 184mg / kg, and the total lead content is 45.2mg / kg.
[0046] Suaeda salsa seeds tested were collected from the wild.
[0048]Medium: potato dextrose (PDA) medium, weigh 200 grams of potatoes, wash and cut into pieces, add deionized water to boil for 30 minutes, filter the supernatant, add 20 grams of glucose, and dilute to 1000ml with deionized water, Sterilize at 121°C for 20 minutes. For solid medium, add 15-20 grams of agar.
[0049] Preparation of Trichoderma viride F7 spore suspension: Activate Trichoderma viride F7 on a PDA slant, produce spores after about 6 days, add 6m...
Embodiment 2
[0060] Soil for test: farmland soil in a town of Pingdu City, its basic physical and chemical properties are: pH 7.08 (soil-water ratio 1:2.5), organic matter 14.9g / kg, cation exchange capacity 18.6cmol / kg, total copper content 67.5mg / kg kg, the total cadmium content is 4.15mg / kg, the total zinc content is 418mg / kg, and the total lead content is 276mg / kg.
[0061] Suaeda salsa seeds tested were collected from the wild.
[0063] Medium: potato dextrose (PDA) medium, weigh 200 grams of potatoes, wash and cut into pieces, add deionized water to boil for 30 minutes, filter the supernatant, add 20 grams of glucose, and dilute to 1000ml with deionized water, Sterilize at 121°C for 20 minutes. For solid medium, add 15-20 grams of agar.
[0064] Preparation of Trichoderma viride F7 spore suspension: Activate Trichoderma viride F7 on a PDA slant, produce spores after about 6 days, add 6m...
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Abstract
The invention relates to a method for remedying heavy metal contaminated soil with combination of trichoderma virens and suaeda and belongs to the technical field of soil remediation. The method comprises the following steps: colonizing trichoderma virens strain F7 in rhizosphere of suaeda to build a trichoderma virens strain F7-suaeda alternation carrier; colonizing the trichoderma virens strain F7-suaeda alternation carrier in the heavy metal contaminated soil for remedying the soil; harvesting overground parts of the suaeda plants after colonizing for 120-136 days and completing soil remediation. Through the method, the trichoderma virens strain F7-suaeda alternation carrier is used for treating the heavy metal contaminated soil, so that the remediation efficiency of copper-cadmium-zinc-lead compound contaminated soil is improved; the phenomenon of synergic effect of accelerating remediation exists between the microorganisms and plants; meanwhile, through the trichoderma virens strain F7-suaeda alternation carrier, the microorganisms in the soil are increased; the rhizosphere micro-ecological environment of the plants can be improved; when the trichoderma virens strain F7-suaeda alternation carrier is used for treating the heavy metal contaminated soil, a good ecological effect can be achieved; the trichoderma virens strain F7-suaeda alternation carrier is applied to remediation of the heavy metal compound contaminated soil.
Description
technical field [0001] The invention belongs to the technical field of soil remediation, and in particular relates to a method for jointly remediating heavy metal-contaminated soil by using Trichoderma viride and Suaeda salsa. Background technique [0002] Soil heavy metal pollution is one of the major environmental problems facing the world. Cd has attracted widespread attention because of its strong biological toxicity and strong "three-induced" effects. The traditional treatment methods for heavy metal pollution are mostly physical and chemical methods. Such as chemical precipitation method, ion exchange method, reverse osmosis method, extraction method, activated carbon adsorption method, etc., they have their own advantages, but there are also disadvantages such as large investment, high energy consumption, easy to cause secondary pollution, and difficult operation. [0003] Compared with traditional heavy metal pollution remediation methods, phytoremediation has the a...
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