Multicolor Fluorescence Detection Device

Abstract

A multi-color fluorescence detection device includes a fluorescence tank, a light emitting unit, a detection unit and an actuating unit. The fluorescent tank is used for accommodating the fluorescent mixture. The light emitting unit includes a light source and a first adjustable filter, wherein the light source provides broadband illumination, and the first adjustable filter allows light beams of different passbands to pass based on different positions or angles to excite the fluorescent mixture. The detection unit includes a second adjustable optical filter and a photodetector, wherein the second adjustable optical filter allows light beams with different passbands emitted from the fluorescent mixture to pass through based on different positions or angles, and the photodetector receives the fluorescent signal and convert it into an electronic signal. The actuating unit is structured to drive the first and second tunable optical filters to different positions or angles, so as to respectively control the change of the passband of the first and second tunable optical filters.

Description

technical field [0001] The invention relates to a fluorescence detection device, in particular to a multi-color fluorescence detection device. Background technique [0002] In recent years, the demand for obtaining a large amount of specific fragment DNA for different purposes has been increasing. Among the current DNA sequencing technologies, the polymerase chain reaction (Polymerase Chain Reaction, PCR) is one of the most economical and rapid technologies, which can Amplify one billion copies of target DNA fragments in a short time. PCR technology has been widely used, such as selective DNA isolation for genetic identification, forensic analysis for archaeological analysis of ancient DNA, medical applications of genetic testing and tissue typing, rapid and specific detection of infectious diseases in hospitals and research institutions diagnostics, environmental hazard monitoring for food safety, and fingerprinting for criminal investigations. PCR technology only needs t...

AI Technical Summary

Problems solved by technology

However, multi-color fluorescence detection devices for portable qPCR systems are difficult to develop in the market due to their cost, size, and high signal-to-noise ratio (SNR) and have not yet been resolved.

Also, due to the limited choice of filter combinations in traditional multicolor qPCR systems, users are limited to only a few fluorophores

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