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Application of anti-CarP (Carbamylated protein) antibodies as systemic lupus erythematosus (SLE) diagnostic marker

A systemic technology for lupus erythematosus, applied in the field of clinical medicine, can solve the problems of low positive rate and poor specificity, and achieve the effect of increasing positive rate, good specificity, and broad clinical diagnostic application value

Inactive Publication Date: 2019-07-16
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of low positive rate or poor specificity in the existing SLE immunodiagnosis, and to provide a new use of SLE diagnostic markers. Another purpose of the present invention is based on the use of the markers in SLE A diagnostic reagent for SLE is provided

Method used

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  • Application of anti-CarP (Carbamylated protein) antibodies as systemic lupus erythematosus (SLE) diagnostic marker
  • Application of anti-CarP (Carbamylated protein) antibodies as systemic lupus erythematosus (SLE) diagnostic marker
  • Application of anti-CarP (Carbamylated protein) antibodies as systemic lupus erythematosus (SLE) diagnostic marker

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The preparation of embodiment 1 carbamylated protein

[0029] The vimentin protein is automatically carbamylated in the presence of KOCN (potassium cyanate). The simple steps are as follows:

[0030] 50 μg of human vimentin protein (purchased from Sigma Company) was added to 10 mL of 0.1 M KOCN (PH7.4) solution, and incubated at 37° C. for 17 h. Dialysis was performed at 4°C for 36 hours, during which the medium was changed 5 times. The resulting solution was carbamylated protein (CarP) at 5 μg / ml.

Embodiment 2

[0031] Example 2 Anti-CarP antibody is used as a marker for the detection of SLE

[0032] In this study, enzyme-linked immunosorbent assay (ELISA) was used to detect the anti-CarP antibody in the serum of patients with systemic lupus erythematosus (SLE) and healthy people, so as to evaluate the significance of the antibody in SLE.

[0033] The reagent combination for detecting the anti-CarP antibody in the subject's serum sample includes the following components: CarP protein, 10 equal parts of SLE patient mixed serum as standard serum (for each ELISA plate for each detection), detection antibody (detection Antibody, or enzyme-labeled secondary antibody, is combined with horseradish peroxidase HRP and can specifically recognize anti-CarP antibody), chromogenic substrate (ie, TMB reagent, which can be catalyzed by HRP for color reaction).

[0034] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and...

Embodiment 3

[0070] Embodiment 3Fib makes the clinical detection of carbamylated protein

[0071] Using Fib (ie Fibrinogen, fibrinogen) as a raw material, referring to the method of Example 1, carbamylation reaction was carried out to obtain carbamylated protein (CarP).

[0072] The carbamylated protein was used as an antigen to detect antibodies in 106 cases of SLE and 73 cases of normal people by referring to the method of Example 2. See the test results image 3 , the results showed that the anti-CarP antibody level of SLE patients was significantly higher than that of the healthy control group, p<0.001, the difference was statistically significant.

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Abstract

The invention discloses application of anti-CarP (Carbamylated protein) antibodies as a systemic lupus erythematosus (SLE) diagnostic marker and belongs to the technical field of immune medicine. Theanti-CarP antibodies in a systemic lupus erythematosus patient are clearly increased. Through utilization of direct relevance between the anti-CarP antibodies and systemic lupus erythematosus, the systemic lupus erythematosus can be diagnosed accurately. The anti-CarP antibodies have wide clinical diagnosis application value. The SLE patient can be identified well from people who are healthy through detection of other detection methods. The anti-CarP antibodies are taken as supplement of other specific antibodies of the SLE, so an SLE detection positive rate is improved. The anti-CarP antibodies are related to various clinical and laboratory indexes, this hints that the anti-CarP antibodies may effectively predict disease activity.

Description

technical field [0001] The invention relates to the technical field of clinical medicine, in particular to the application of an anti-carbamylated protein (CarP) antibody as a diagnostic marker for systemic lupus erythematosus. Background technique [0002] Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by multiple system and organ damage accompanied by a large number of autoantibodies. The number of patients with this disease in my country is as high as 9.1 million, mostly in young women, and the disease progresses rapidly. If it is not controlled, it can quickly develop into multiple organ failure, and the 5-year survival rate is only 73% (Lee SJ, Silverman E, Bargman JM. The role of antimalarial agents in the treatment of SLE and lupus nephritis. Nat Rev Nephrol 2011; 7:718–729), is one of the major death diseases that endanger human life. [0003] However, the pathogenesis of SLE is not completely clear at present, the condition is comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 栗占国李英妮刘燕鹰胡凡磊唐素玫贾汝琳相晓红
Owner PEOPLES HOSPITAL PEKING UNIV
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