Preparation method of separation tube for separating tumor cells

Abstract

The invention provides a preparation method of a separation tube for separating tumor cells, and belongs to the technical field of cell separation. The preparation method comprises the following stepsof firstly adding separation gel to the separation tube, performing centrifuging so as to enable the separation gel to sink to the bottom of the tube, then adding separation mediums, then slowly adding body cavity hydrops or anticoagulation whole blood to an upper layer of the separation mediums, performing centrifuging at room temperature, firstly sucking and discarding liquid at the topmost surface of the separation tube so as to remove cell debris, then dumping residual liquid on the separation gel layer into a collecting tube, then performing centrifuging at room temperature, performing cell re-suspending with PBS so as to obtain the tumor cells, wherein the density of the separation gel is 1.06-1.08g / mL, and the density of the separation mediums is 1.05-1.06g / mL. The preparation method disclosed by the invention can be used for separating and circulating the tumor cells from the tumor cells in the body cavity hydrops, the preparation method is simple, convenient and efficient, and the purity of the separated tumor cells is high.

Description

technical field [0001] The invention belongs to the technical field of cell separation, and in particular relates to a method for preparing a separation tube for separating tumor cells. Background technique [0002] Body cavity effusion refers to the effusion in the cavities around the organs of the human body, mainly including the pleural cavity, abdominal cavity, pelvic cavity and subarachnoid space. Body cavity effusions are associated with many types of malignancies, such as liver cancer, lung cancer, breast cancer, Koposi's sarcoma, and lymphoma, among others. Isolation and identification of tumor cells and their gene mutation types in body cavity fluid have important clinical significance for disease diagnosis, disease assessment and formulation of treatment strategies. The cell types in the body cavity fluid of tumor patients are relatively complex, mainly including red blood cells, white blood cells, and mesothelial cells, etc., and the number of tumor cells is rela...

AI Technical Summary

Problems solved by technology

However, directly using Percoll to separate circulating tumor cells or body cavity effusion tumor cells by density centrifugation method, while harvesting tumor cells, it will inevitably be polluted by other cells, and tumor cells with higher purity cannot be obtained.

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