Super-sensitive chemiluminescent HRP substrate solution and preparation method thereof

Abstract

The invention discloses a super-sensitive chemiluminescent HRP substrate solution, which is prepared by compounding a solution A and a solution B in equal volume. The solution A is 1000 mL, which hasa pH value of 9.0 and contains 24.23 g of Tris, 2.060 g of Phenothiazine-10-yl-propylsulfonic acid sodium salt, 0.37 g of 4-dimethylaminopyridine, 0.44 g of luminol, and 1 mL of 1 mol / L NaOH solution,the balance being hydrochloric acid and pure water. The solution B is 1000 mL, which has a pH value of 3.5-5.0, and contains 1.175 g of carbamide peroxide, and 2.875 mL of glacial acetic acid, the balance being pure water and 5 mol / L NaoH solution. The method adopts the international most advanced sensitization technology in the prior art and carries out secondary sensitization, so that the substrate solution has high sensitivity which is higher than that of foreign products of Thermo Pierce; and the light is stable, the background is lower, the pg level can be reached, the using amount of antibodies and samples is greatly saved, and the method is particularly suitable for detecting the low-abundance protein content.

Description

Technical field [0001] The invention relates to the technical field of ultra-sensitive chemiluminescence, in particular to a super-sensitive chemiluminescence HRP substrate solution and a preparation method thereof. Background technique [0002] Chemiluminescence technology is a kind of immunodiagnostic technology, and the difference of immunodiagnostic technology is mainly in the two links of antigen-antibody coating and signal detection. Currently, chemiluminescence HRP substrate solutions on the market are used in scientific research or For medical immunodiagnosis, most of them rely on imports and are expensive. Most of the domestic luminescent liquids are not sensitive, luminous or unstable or damage the polyethylene fiber membrane used in Western blot experiments. Summary of the invention [0003] In order to solve the above-mentioned problems, the present invention provides a super-sensitive chemiluminescence HRP substrate solution and a preparation method thereof. It adopts...

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Problems solved by technology

[0002] Chemiluminescent technology is a kind of immunodiagnostic technology, and the difference of immunodiagnostic technology is mainly in the two links of antigen antibody coating and signal detection. When used in medical immunodiagnosis, most of them rely on imports and are expensive, while most of the domestic luminescent liquids are not sensitive, luminescence is unstable, or there is damage to the polyethylene fiber membrane used in Western blot experiments.

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