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Method for inducing coralline planula larvae to improve adhesion capability

A technology for floating larvae and attachment ability, which is applied in the field of inducing coral floating larvae to improve the adhesion ability, which can solve problems such as the structural formula is not completely determined, and achieve the effect of improving the level of ecological conservation and promoting attachment and development

Active Publication Date: 2019-07-23
SHENZHEN INSTITUTE OF INFORMATION TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many kinds of known alkaloids, about 10,000 kinds, and some structural formulas have not been fully determined

Method used

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  • Method for inducing coralline planula larvae to improve adhesion capability
  • Method for inducing coralline planula larvae to improve adhesion capability
  • Method for inducing coralline planula larvae to improve adhesion capability

Examples

Experimental program
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Effect test

preparation example Construction

[0052] The preparation method comprises the following steps:

[0053] S1) Weigh bromopyrrole alkaloid (-)-Agelastatin A and polycaprolactone (PCL) and dissolve them in an organic solvent, then fully ultrasonically oscillate and mix to obtain an oil phase; wherein, bromopyrrole alkaloid (-)-Agelastatin The mass ratio of A to polycaprolactone is 40-50:280-320, preferably 43-48:290-310, preferably 44-46:295-305, more preferably 45:300; the organic solvent is preferably is dichloromethane;

[0054] S2) Take the above oil phase and slowly add it dropwise into the polyvinyl alcohol aqueous solution, stir at a high speed, and emulsify in a sealed environment. Preferably, the emulsification speed is 250-350r.min -1 , after closed emulsification for 50-70min, reduce the speed to 80-120r.min -1 ;

[0055] S3) continuously stirring to volatilize the organic solvent dichloromethane to solidify the microspheres to obtain an emulsion;

[0056] S4) The emulsion is separated by centrifuga...

Embodiment 1

[0064] The preparation method of bromopyrrole alkaloid polycaprolactone microspheres described in this embodiment comprises the following steps:

[0065] 1) Accurately weigh 45 mg of bromopyrrole alkaloid (-)-Agelastatin A technical material and 300 mg of polycaprolactone (PCL) in 12 mL of dichloromethane, then fully ultrasonically oscillate and mix to obtain the oil phase;

[0066] 2) Use a syringe to absorb the oil phase and slowly drop it into 100mL of polyvinyl alcohol aqueous solution (2% mass fraction), stir at high speed, and the emulsification speed is 300r.min -1 ;

[0067] 3) After closed emulsification for 60 minutes, reduce the speed to 100r.min -1 ; Stir continuously for 3h to volatilize dichloromethane to solidify the microspheres;

[0068] 4) The emulsion is centrifuged at 1000r.min-1, and the obtained solid is washed with distilled water, and again at 1000r.min -1 Centrifuged, repeatedly washed and centrifuged 3 times; placed in a low-temperature freeze-vacu...

Embodiment 2

[0072] Adding the bromopyrrole alkaloid (-)-Agelastatin A to the live coralline algae as the culture substrate

[0073] Effects on the attachment ability of coral plankton larvae.

[0074] 1.1 Collection and cultivation of coral larvae

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Abstract

The invention relates to a method for inducing coralline planula larvae to improve adhesion capability. The method includes the following steps: taking a living shell-shaped coralline algae as a larval adhesion culture substrate; uniformly spraying bromopyrrole alkaloid (-)-Agelastatin A polycaprolactone microspheres on the surface of the shell-shaped coralline algae; or smearing an organic solvent solution of the bromopyrrole alkaloid (-)-Agelastatin A on the living shell-shaped coralline algae; and placing the coralline planula larvae on the substrate of the culture container formed by the above treatment and culturing in seawater. The invention further provides the bromopyrrole alkaloid polycaprolactone microspheres and a preparation method thereof. According to the method, the living shell-shaped coralline algae is taken as the larval adhesion culture substrate, and the bromopyrrole alkaloid (-)-Agelastatin A polycaprolactone microspheres are added, and thus the adhesion and development of the coralline planula larvae on the substrate can be well promoted, so that the ecological conservation level of corals and the ecological restoration technology level of coral reefs are improved.

Description

technical field [0001] The invention belongs to the fields of marine biotechnology and ecological environment protection, and in particular relates to a method for inducing coral floating larvae to improve their attachment ability. Background technique [0002] As we all know, the coral reef ecosystem is one of the ecosystems with the most complex biodiversity and the highest production capacity on the earth. Marine plants also use coral reefs as habitats. The coral reef ecosystem not only breeds rich organisms, but also is a very important fishery resource and tourism resource. As one of the ecosystems with the highest ecological and economic value in the world, coral reefs provide human society with huge economic, social and cultural value and ecological service functions. However, in the past 20 years, global coral reefs have experienced unprecedented damage under the influence of human activities and global changes. Global coral reefs are facing a trend of degradation,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A10/26Y02A40/81
Inventor 聂磊
Owner SHENZHEN INSTITUTE OF INFORMATION TECHNOLOGY
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