Application of licochalcone-A in preparation of anti-haemophilus-parasuis drug

A technology of Haemophilus suis and licorice chalcone is applied in the directions of antibacterial drugs, ketone active ingredients, etc., and can solve problems such as hindering the development and utilization of licorice chalcone A

Active Publication Date: 2019-07-23
FEED VETERINARY MEDICINE RES INST SHANXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research report on the anti-Haemophilus parasuis of licochalcone A at home and abroad, which seriously hinders the further development and utilization of licochalcone A as a bacterial inhibitor. The research and application of haemophilus is imminent

Method used

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  • Application of licochalcone-A in preparation of anti-haemophilus-parasuis drug
  • Application of licochalcone-A in preparation of anti-haemophilus-parasuis drug
  • Application of licochalcone-A in preparation of anti-haemophilus-parasuis drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 In vitro inhibitory effect experiment of licochalcone A on Haemophilus parasuis

[0019] 1. Effect of licochalcone A on the growth rate of Haemophilus parasuis

[0020] The activated Haemophilus parasuis was inoculated in TSA liquid medium (containing newborn bovine serum and NAD), and cultured overnight at 37°C with shaking at 200r / min; then the bacterial liquid (10 6 CFU / mL) were respectively transferred to fresh liquid medium with final concentrations of 10 μg / mL, 20 μg / mL, 50 μg / mL, 100 μg / mL and 200 μg / mL licochalcone A, at 37° C culture, take out 100 μL of bacterial solution every 1 h and serially dilute it 10 times with sterile PBS, then take 100 μL of bacterial solution and spread it on the solid medium containing newborn bovine serum and NAD TSB, repeat 3 times for each dilution, and cultivate overnight After counting, draw the growth curve;

[0021] The effect of licochalcone A on the growth rate of Haemophilus parasuis as follows: figure 1 As s...

Embodiment 2

[0025] Example 2 The effect of licochalcone A on the growth and intestinal flora of mice

[0026] 1. Effect of licochalcone A on the growth of mice

[0027] 72 mice were randomly and equally divided into low-dose group, middle-dose group, high-dose group and control group. Low-dose group: Orally administered licochalcone A to mice at a dose of 1 mg / kg, 0.2 mL each time. Medium-dose group: Orally administered licochalcone A to mice at a dose of 10 mg / kg, 0.2 mL each time. High-dose group: Orally administered licochalcone A to mice at a dose of 50 mg / kg, 0.2 mL each time. Control group: normal feeding. The administration was administered by intragastric administration once a day for 7 consecutive days, the clinical symptoms of the mice were observed, the body weight of the mice was measured on the 3rd day, 5th day and 7th day respectively, and 6 mice were sacrificed respectively, and the lungs, heart, Kidney, thymus and spleen, calculate organ index, organ index = mouse orga...

Embodiment 3

[0037] Example 3 Inhibitory effect of licochalcone A on the proliferation of Haemophilus parasuis in mice

[0038] 1. The median lethal dose (LD) of Haemophilus parasuis to mice 50 ) determination

[0039] 72 mice were randomly divided into 6 groups (5 experimental groups and 1 blank group), and the freshly cultured Haemophilus parasuis was serially diluted 5-fold with sterile PBS, and an appropriate dilution was selected, and 200 μL / peritoneal Infect mice. Observe the state of the mice until there is no mouse death, record the death situation, and calculate the LD of Haemophilus parasuis on the mice according to the Bliss method 50 The results showed that the mice died on the 3rd day after the intraperitoneal injection of Haemophilus parasuis, and the death of the mice was continuously observed and recorded until no death occurred within 5 days. The LD50 of bacillus H12L1 to mice was 8.72×10 7 CFU / mL.

[0040] 2. Effect of licochalcone A on the proliferation of Haemophil...

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Abstract

The invention discloses application of licochalcone-A in preparation of an anti-haemophilus-parasuis drug. The anti-haemophilus-parasuis drug comprises the following components: licochalcone-A, pharmaceutically acceptable excipients, and auxiliary substrates. Tests have proven that inhibitory effects of licochalcone-A offielddifferent concentrations on haemophilus parasuis in vitro are not significantly different; and licochalcone-A of different concentrations has no effect on formation of bacterial biofilm. In vivo test results have suggested that immune organ indexes of mice are appropriately enhanced by licochalcone-A with abundance and structure of intestinal flora in the mice improved, so that, the licochalcone-A is capable of improving ability of the mice to eliminate haemophilus parasuis, enhancing ability of infection protection of the mice as well as delaying time of death of the mice infected with haemophilus parasuis.

Description

technical field [0001] The invention belongs to the technical field of antibacterial application of active ingredients, and in particular relates to the application of licochalcone A in the preparation of anti-Haemophilus parasuis medicaments. Background technique [0002] Haemophilus parasuis ( Haemophilus parasuis ) is a small Gram-negative bacillus that inhabits the upper respiratory tract of pigs, causing Haemophilus parasuis disease or Glasser's disease, with typical clinical symptoms of polyserositis, arthritis or sepsis systemic disease. Pigs aged 2 weeks to 4 months are the main infection targets of Haemophilus parasuis, and the highest morbidity and mortality can reach 40% and 50%, respectively. Due to the existence of multiple serotypes of Haemophilus parasuis, the cross-immunity effect of Haemophilus parasuis vaccine is not ideal. At present, there is no effective measure to prevent and control the outbreak and prevalence of Haemophilus parasuis. [0003] In th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P31/04
CPCA61K31/12A61P31/04
Inventor 杨裕杨志强杨春雷贾永鑫
Owner FEED VETERINARY MEDICINE RES INST SHANXI ACADEMY OF AGRI SCI
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