Unlock instant, AI-driven research and patent intelligence for your innovation.

siRNA, recombinant vector and application thereof for reducing rogdi gene expression

A technology of gene expression and nucleotide sequence, which is applied to siRNA, recombinant vector and its application fields to reduce the expression of ROGDI gene, and can solve the problem that the biological function of ROGDI has not been reported.

Active Publication Date: 2021-10-15
乾元康安(苏州)生物科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the biological function of ROGDI has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • siRNA, recombinant vector and application thereof for reducing rogdi gene expression
  • siRNA, recombinant vector and application thereof for reducing rogdi gene expression
  • siRNA, recombinant vector and application thereof for reducing rogdi gene expression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example is used to illustrate the high expression of ROGDI in breast cancer cell lines.

[0045] RNA extraction and reverse transcription quantitative PCR (RT-qPCR).

[0046] 1. Extraction of total RNA: this embodiment is carried out at low temperature. Cell culture was carried out in a 6-well plate, remove the medium, rinse with PBS 3 times, add Trizol 1000μl to each well, shake for 10min; collect into 1.5ml centrifuge tube, add 200μl chloroform to each tube, mix vigorously for 30sec, let stand for 15min , centrifuge at 12000rpm at 4°C for 15min; gently pipette 400μl of the upper layer into another new centrifuge tube, add an equal volume of isopropanol, gently invert and mix well, and centrifuge at 12000rpm at 4°C for 10min; discard the supernatant, and add 1ml of 75% alcohol to wash the precipitate centrifuge at 12,000 rpm at 4°C for 10 min; discard the supernatant as much as possible, and dry at room temperature for 10 min, add 10 μl of RNase-free water to eac...

Embodiment 2

[0054] This example is used to illustrate that knocking down the expression of ROGDI gene can inhibit the proliferation and / or growth of breast cancer.

[0055] 1. Preparation of RNAi lentiviral clones

[0056] 1. Target design

[0057] For the ROGDI gene sequence, according to the RNAi sequence design principles, design 3 RNAi target sequences, the nucleotide sequences of the 3 siRNAs of the ROGDI gene, and the nucleotide sequences of the negative control (NC). The names of the three siRNAs and the negative control are ROGDI-si-1a, ROGDI-si-1b, ROGDI-si-2a, ROGDI-si-2b, ROGDI-si-3a, ROGDI-si-3b, NC-si -a and NC-si-b are sequences designed for the negative control group. See Table 2 for details.

[0058] Table 2 Nucleotide sequences of 3 RNA interference targets (siRNA) and negative controls

[0059]

[0060]

[0061] Table 3 shows the nucleotide sequences of the three shRNAs used in the examples. The shRNAs used in the examples are ROGDI-sh-1a, ROGDI-sh-1b, ROGDI-sh...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical fields of molecular biology and genetic engineering, and relates to an siRNA for reducing the expression of ROGDI gene, a recombinant carrier and application thereof. Specifically, it relates to an siRNA that specifically reduces ROGDI gene expression. Since the siRNA provided by the invention can specifically reduce the expression of ROGDI gene, the siRNA can be applied to the preparation of medicaments for treating and / or preventing breast cancer.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and genetic engineering, and more specifically, relates to a siRNA that reduces the expression of ROGDI gene, a shRNA corresponding to the siRNA, a DNA encoding the shRNA, a recombinant vector including the DNA, including the recombinant The recombinant lentivirus of the vector includes the siRNA, shRNA, DNA encoding shRNA, the host cell of the recombinant vector and the recombinant lentivirus, and their applications. Background technique [0002] Breast cancer is one of the most common malignant tumors in women worldwide, and its incidence rate and mortality rate rank first among all types of cancer. Although the incidence of breast cancer in my country is relatively low, the population base is large, the overall number of patients is large, the incidence rate is increasing year by year, and the age of onset is younger. Breast cancer exhibits great complexity and heterogeneity in term...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/867A61K31/7088A61P35/00A61P15/14
CPCA61P15/14A61P35/00C12N15/113C12N15/86C12N2310/14C12N2740/15043C12N2310/531
Inventor 张虎杜欣娜杨留才胡明李春明
Owner 乾元康安(苏州)生物科技有限公司