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Method for judging endometrial receptivity and application thereof

An endometrial and receptive technology, applied in the field of biomedicine, can solve problems such as implantation failure and embryo implantation failure

Active Publication Date: 2019-07-23
YIKON GENOMICS (SUZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the field of in vitro fertilization-embryo transfer (IVF-ET), the time node of the implantation window period is not accurate for women who have repeatedly failed implantation or suffer from other secondary infertility. If it is based on menstrual cycle or ovulation day Estimated implantation window period, there will be a great risk of transplant failure
Although the specific mechanism of endometrial receptivity is still unclear, it is clear that poor endometrial receptivity is one of the important reasons for embryo implantation failure in in vitro fertilization-embryo transfer (IVF-ET)

Method used

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  • Method for judging endometrial receptivity and application thereof
  • Method for judging endometrial receptivity and application thereof
  • Method for judging endometrial receptivity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0196] Example 1 Sample Pretreatment and RNA Extraction

[0197] Obstetricians and gynecologists or professionals with qualifications for obtaining biopsy samples will take samples. The samples include more than 5 mg of fundus endometrial tissue, more than 10 ul of uterine cavity fluid or more than 10 ul of other reproductive endocrine-related body fluids, and more than 5 mg of vaginal exudates. After the biopsy is completed, completely infiltrate the tissue, exfoliation or liquid in RNA preservation solution (about 20uL RNA Later) as soon as possible. Before sample transportation, samples were stored in -20°C or -80°C refrigerators.

[0198] Use Qiagen RNeasy Micro Kit produced by Qiagen to extract the RNA of endometrial tissue, the specific method is as follows:

[0199] 1. Experiment preparation: Use RNase remover and nucleic acid remover to wipe hands, pipettes and experimental benchtops (please note that all extraction steps should be performed in an RNase-free area).

...

Embodiment 2

[0219] Example 2 RNA reverse transcription and cDNA amplification reaction

[0220] 1. Experiment preparation: Use RNase remover and nucleic acid remover to wipe hands, pipettes and ultra-clean bench tops. Prepare pipette tips free of RNase and nucleic acid contamination, 1.5mL EP tubes and 0.2mL PCR tubes in an ultra-clean room, and turn on the ultra-clean bench for UV irradiation for 30 minutes (please note that steps 2 to 11 should be performed on a tube free of RNase and nucleic acid contamination. Ultra-clean Taichung).

[0221] 2. Thaw RNA samples and Lysis Buffer (provided with the kit) on ice, vortex after thawing, centrifuge briefly and put on ice for later use.

[0222] 3. Take 2uL RNA from each sample and place it in a 1.5mL EP tube. According to the determined RNA concentration of the sample, dilute the RNA sample to about 5 ng / uL with RNaseFree water.

[0223] 4. Add 1.5uL Lysis Buffer and 3.5uL diluted RNA sample to the 0.2mL PCR tube in sequence.

[0224] 5. S...

Embodiment 4

[0256] The quality inspection of embodiment 4 cDNA product

[0257] Take 1 μl of the purified cDNA amplification product after reasonable dilution for detection. For operating instructions, see the HighSensitivity DNA Chip operation manual. For results, see figure 1 .

[0258] In general, the sample cDNA amplification products are distributed at 400-10000bp, and the main peak is located at about 2000bp, which is determined by figure 1 It can be seen that the cDNA composite quality requirements used in this application.

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Abstract

The present invention provides a method for judging endometrial receptivity and an application thereof, and specifically provides the method for judging the endometrial receptivity and a marker for judging endometrial receptivity statuses. The marker can significantly reduce an error rate of the endometrial receptivity judgment.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for judging endometrium receptivity and its application. Background technique [0002] In the process of human reproduction, fertilized eggs are positioned, adhered, and implanted in the mother's uterus, and finally develop into a mature fetus. The implantation process has an important impact on successful pregnancy. Successful clinical pregnancy requires good embryos in addition to high-quality embryos. The endometrial receptivity (endometrial receptivity, ER), the synchronous development of endometrium and embryo. Endometrial receptivity refers to the ability of the endometrium to accept embryos. The endometrium allows embryos to implant only in a short and specific period. This period is called the "implantation window period". For adult women , which is equivalent to the 20th-24th day of the menstrual cycle or the 6-8th day after ovulation. [0003] In the field of in vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q1/6876G16B25/10G01N2800/367
Inventor 胡春旭董鑫陆思嘉胡旻涛
Owner YIKON GENOMICS (SUZHOU) CO LTD
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