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A method for improving the adsorption capacity of powdery chitin to enzyme protein and its application

A technology of chitin and chitin-binding domain, which is applied in the field of chitin fiber preparation, can solve the problems of environmental pollution, harsh preparation conditions, long preparation period and the like, and achieves improved protein adsorption performance, short preparation period and simple steps. Effect

Active Publication Date: 2021-10-22
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the process of preparing chitin nanofibers involved in the current patent, chemical reagents such as strong acid and strong alkali need to be involved, which seriously pollutes the environment.
At the same time, the preparation conditions are harsh and the preparation cycle is long

Method used

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  • A method for improving the adsorption capacity of powdery chitin to enzyme protein and its application
  • A method for improving the adsorption capacity of powdery chitin to enzyme protein and its application
  • A method for improving the adsorption capacity of powdery chitin to enzyme protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Screening of chitin powder

[0036] A 100-mesh sieve was used to sieve chitin powder with a uniform diameter of 100 mesh from chitin powders with different particle sizes. The chitin powder was purchased from Aladdin (Shanghai) Co., Ltd.

Embodiment 2

[0037] Example 2 Preparation of Chitin Suspension

[0038] The 100-mesh chitin powder sieved in Example 1 was washed three times with pure water, and dried in an oven at 65°C. Weigh 2 grams of dried chitin powder and add distilled water until the final concentration of chitin powder is 2% to prepare chitin suspension.

Embodiment 3

[0039] Example 3 Preparation of chitin nanofibers

[0040] With the chitin suspension obtained in Example 2, the feed rate of the high-pressure homogenizer is set to be 15L / h, and the pressure parameter of the high-pressure homogenizer is set at this feed rate to be 600bar, and the chitin suspension After 5 cycles of average treatment, the suspension of chitin nanofibers was prepared. figure 1 and figure 2 The SEM images of chitin before and after treatment are respectively. It can be clearly compared in the figure that the chitin treated with high pressure homogenization has finer nano whiskers. figure 1 The surface of chitin under medium SEM has a rough sheet structure with small holes in it, which is not conducive to the adsorption of enzyme proteins; figure 2 The chitin fibers processed by a high-pressure homogenizer under medium SEM have a diameter distribution of 50nm-100nm, and this nanofiber structure can absorb more enzyme proteins.

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Abstract

The invention discloses a method for improving the adsorption capacity of powdery chitin to enzyme protein and its application. After the chitin powder is processed by a high-pressure homogenizer, chitin fibers with a fiber diameter of nanometer scale are obtained, which are used to adsorb proteins with chitin binding domains ( cBD ) fusion enzyme, after a series of condition optimization, it was found that the adsorption effect of the pretreated chitin fiber on the enzyme protein was greatly improved compared with the untreated chitin powder. The invention provides a method for improving the adsorption capacity of conventional powder chitin to enzyme protein. The method is environmentally friendly and does not involve the use of strong acid and alkali. The prepared chitin fiber has small diameter, large relative area, and protein-binding ability. It has strong characteristics and has good application and development prospects in the field of enzyme immobilization materials.

Description

technical field [0001] The invention relates to the technical field of chitin fiber preparation, in particular to a method for improving the adsorption capacity of chitin powder to enzyme protein and its application. Background technique [0002] Chitin (C 8 h 13 o 5 N)n, also known as chitin or chitin, is an important polysaccharide that is second only to cellulose in nature. It is widely found in the shells of shrimps, crabs, insects, and the exoskeletons of some marine molluscs, and has a wide range of sources. Chitin is a polymer linked by N-acetylglucosamine through α-1,4 glycosidic bonds. It has a unique molecular structure, endowing it with outstanding properties such as non-toxic, biocompatible, and biodegradable. It can be made into various materials, such as film products, materials, gels, scaffolds, nanoparticles and nanofibers, etc., so that it can be used in many fields such as medical sutures, antibacterial, health care additives, etc. Among them, chitin na...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/28C12N11/10
CPCB01J20/24B01J20/28007B01J20/28023B01J2220/4825C12N11/10
Inventor 陈可泉周宁张阿磊魏国光杨赛莫晓芳王莹莹欧阳平凯
Owner NANJING TECH UNIV
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