Cosmetic composition comprising fermented coconut sugar extract and manufacturing method thereof
A technology of cosmetic materials and fermentation products, which is applied in the field of cosmetic material compositions and can solve the problems of limited types
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[0035] According to an embodiment of the present invention, the coconut sugar fermentation product is prepared by the following preparation method, the method comprising: the step of cultivating lactic acid bacteria; the step of inoculating the cultured lactic acid bacteria strains into the coconut sugar solution; and cultivating the inoculated Coconut sugar solution of lactic acid bacteria and a step of fermentation.
[0036] According to an embodiment of the present invention, the cosmetic material composition can be prepared by the following preparation method of the cosmetic material composition comprising the coconut sugar fermentation product of the present invention as an active ingredient.
[0037] According to an embodiment of the present invention, compared with the coconut sugar fermented products fermented by using unfermented coconut sugar extracts and common fermenting microorganisms such as yeast, the filaggrin (filaggrin) of the coconut sugar lactic acid bacteri...
Embodiment 1
[0057] Preparation of coconut sugar lactic acid bacteria fermentation product of the present invention
[0058] Preparation of Coconut Sugar 5% by Weight Lactic Acid Bacteria Fermentation Product
[0059] In order to prepare the cosmetic composition containing the coconut sugar fermentation product of the present invention, commercially available coconut sugar (Republic of Korea, HEDEN Co., Ltd., www.heden.co.kr) was purchased, and the coconut sugar fermentation product was prepared as follows. As the fermentation microorganism, lactic acid bacteria (KCCM 12203, Lactobacillus sp.) purchased from the Korean Microorganism Collection were used.
[0060] The lactic acid bacteria were pre-cultivated in MRS broth (BD, USA) so that the initial bacterial count was 1.0×10 5 ~5.0×10 5 cfu / ml. After putting 95 g of distilled water and 5 g of palm sugar into an Erlenmeyer flask and dissolving it completely, it sterilized at 121 degreeC for 15 minutes, and cooled to room temperatur...
Embodiment 2
[0074] Evaluation of Filaggrin expression in lactic acid bacteria fermentation products according to coconut sugar concentration
[0075]In order to confirm the impact of different concentrations of coconut sugar on the skin moisturizing and barrier strengthening effect of the coconut sugar lactic acid bacteria fermentation product of the present invention, the sample prepared according to the embodiment to and comparative example 1 is subjects, and the relative mRNA expression levels of Filaggrin, a skin barrier and moisturizing factor, were determined as described below.
[0076] HaCat, which is a human keratinocyte cell line, was purchased from America type culture collection (ATCC, Rockville, MD, USA), and was added with 10% fetal bovine serum (FBS, ATCC, Rockville, MD, USA) and 1% antibacterial-antibiotic Fungal agents (AA, ATCC, Rockville, MD, USA) in DMEM (Hyclone, Logan, UT, USA) at 37°C, 5% CO 2 cultured in a conditioned incubator.
[0077] In order to confirm t...
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