Saccharomyces cerevisiae engineering strain for achieving high yield of pyruvic acid and fermentation method of strain

Abstract

The invention discloses a saccharomyces cerevisiae genetic engineering strain for achieving high yield of pyruvic acid. The strain is classified and named as saccharomyces cerevisiae XY-156A and preserved in the China General Microbiological Culture Collection Center, and the preservation number of the strain is CCTCC M 2019129. The saccharomyces cerevisiae genetic engineering strain XY-156A can utilize a large amount of glucose for fermentation production of the pyruvic acid, fed-batch fermentation is conducted for 76 hours, the concentration of the pyruvic acid in fermentation liquid can reach 105 g / L, for the yield of the pyruvic acid, 0.5 gram of the pyruvic acid is obtained through per gram of the glucose, and the strain has potential application value in fermentation production of the pyruvic acid.

Description

technical field [0001] The invention relates to a high-pyruvate-producing Saccharomyces cerevisiae engineering strain and a fermentation method thereof, belonging to the technical field of fermentation engineering. Background technique [0002] Pyruvic acid (pyruvic acid), a light yellow to yellow transparent liquid, widely exists in the human body, animals, plants and microorganisms, and is an important intermediate product in the process of cell metabolism; pyruvic acid and its salts serve as an important platform Compounds are widely used in chemical, pharmaceutical, agricultural chemicals, food health care and other fields. For example, pyruvic acid and its salts are precursors for the synthesis of important compounds such as L-tyrosine, L-tryptophan, vitamin B6, N-acetyl-D-neuraminic acid, (R)-acetylbenzyl alcohol; In addition, it has potential applications in human health care such as anti-fatigue, increasing endurance, lowering blood fat, and losing weight. [0003]...

AI Technical Summary

Problems solved by technology

For example, most of the starting Saccharomyces cerevisiae strains used in the literature reports are auxotrophic. In the process of using synthetic medium for fermentation, additional nutrients such as essential amino acids need to be added, which increases the difficulty of fermentation control and fermentation costs; In addition, the obtained Engineering strains still have problems such as insufficient pyruvate production and too long fermentation cycle

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