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Preparation method and application of a synovial sarcoma cell line hss-005r

A synovial sarcoma and cell line technology, applied in the field of tumor cell research, can solve the problems of low success rate of cell line establishment, large difference in biological characteristics of clinical synovial sarcoma, and low biodiversity, and achieve high scientific research value and Development significance, high tumor formation rate, good uniformity effect

Active Publication Date: 2020-08-11
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to solve the low biological diversity of existing human synovial sarcoma cell lines, especially the low success rate of cell line establishment, and the biological characteristics of existing cell lines and clinical synovial sarcoma are quite different, etc. Insufficient, providing a new human synovial sarcoma cell line and its establishment method and application

Method used

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  • Preparation method and application of a synovial sarcoma cell line hss-005r
  • Preparation method and application of a synovial sarcoma cell line hss-005r
  • Preparation method and application of a synovial sarcoma cell line hss-005r

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Embodiment 1

[0044] Example 1 is the preparation of the cell line hSS-005R of the present invention.

[0045] Primary culture method: Place fresh synovial sarcoma specimens resected during operation in DMEM high glucose + 10% FBS complete medium in a sterile state, and store them on ice. After rinsing with fresh PBS buffer (containing 1% double anti-penicillin, streptomycin), cut into thin slices, attach the cut tissue pieces to the bottom of the cell culture flask, add appropriate amount of DMEM high sugar +10 Complete culture with %FBS is based on 37°C cell culture incubator with 5% carbon dioxide and placed upright for 1 hour to make the tissue pieces stick firmly to the bottom of the bottle. The upright flask is then gently and slowly tipped over to submerge the adherent tissue pieces with complete medium. There is no need to change the medium, and the cells can usually be observed climbing out of the tissue block after 4-5 days. When the medium turns yellow, replace with a new mediu...

Embodiment 2

[0049] Example 2 is the biological characteristics and application of hSS-005R cells.

[0050] The invention adopts DMEM high-sugar culture medium to cultivate and purify hSS-005R cells, so that the hSS-005R cells can grow and pass stably in vitro for a long time. When the cells are passed on for more than 30 generations, the cell traits are gradually stabilized, and relevant biology, genetics, and tissue source identification are carried out until the 50th generation has the same stable traits. The hSS-005R cells can form tumors in nude mice and have tumorigenicity. The corresponding relationship between the hSS-005R cells and the clinical synovial sarcoma tumor specimens derived from them, and the parental tumors passaged in nude mice can provide a new test for studying the correlation between in vitro, in vivo and clinical anticancer drug sensitivity and drug resistance Material. details as follows:

[0051] a. Morphological observation

[0052] Place the culture flask ...

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Abstract

The invention relates to a preparation method of a synovial sarcoma cell line hSS-005R. The synovial sarcoma cell line is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:C201911; the preparation method comprises primary culture and subculture. The new human synovial sarcoma cell line is established, has stable properties and can achieve stable passage several times. The established human synovial sarcoma cell line has the advantages of a high tumor formation rate, a short incubation period, good homogeneity and the like on the premise of retaining main clinical biological characteristics, so that a synovial sarcoma cell bank is enriched, a synovial sarcoma animal model can be successfully prepared, the animal model can be used for basic research and drug screening, powerful scientific research data is provided for research based on the genetic background of Chinese population, and a new test material is also provided for testing the sensitivity and drug resistance of clinical anticancer drugs during in-vivo research experiments before clinical use of new drugs.

Description

technical field [0001] The invention belongs to the field of tumor cell research, and in particular relates to a preparation method and application of a synovial sarcoma cell line hSS-005R. Background technique [0002] Synovial sarcoma (synovial sarcoma) is a highly invasive and highly malignant tumor. Synovial sarcoma accounts for 5-10% of all soft tissue sarcomas, and accounts for 10-20% of soft tissue tumors in adolescents and young adults. Between 1983 and 2012, the incidence of synovial sarcoma continued to increase from 0.906% to 1.548%. Synovial sarcoma occurs in the extremities, accounting for about 70% of all synovial sarcoma cases. The most common metastatic sites of synovial sarcoma are the lungs, followed by lymph nodes and bone marrow. Synovial sarcoma was originally named for its microstructural similarity to the surrounding synovial tissue when it is found in tissues near joints. However, some studies in recent years have shown that synovial sarcoma can ap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12Q1/02A61K49/00A01K67/027C12R1/91
CPCA01K67/0271A01K2207/12A01K2227/105A01K2267/0331A61K49/0008C12N5/0693C12N2503/02G01N33/5011G01N2500/10
Inventor 黎志宏段智曦黎双庆陈瑞奇谢鹏
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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