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Micro-fluidic chip device for inducing transmembrane migration of cancer cells through fluid shear stress

A microfluidic chip, fluid shear force technology, applied in enzymology/microbiology devices, biochemical cleaning devices, stress-stimulated microbial growth methods, etc., can solve problems such as no longer applicable, and achieve easy production, dynamic Monitoring tumor cell migration, small volume effect

Inactive Publication Date: 2019-08-13
SOUTH CHINA UNIV OF TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] In order to further simulate the transmembrane migration of tumor cells in the blood circulation, traditional experimental techniques such as cell scratching and Transwell will no longer be applicable

Method used

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  • Micro-fluidic chip device for inducing transmembrane migration of cancer cells through fluid shear stress
  • Micro-fluidic chip device for inducing transmembrane migration of cancer cells through fluid shear stress
  • Micro-fluidic chip device for inducing transmembrane migration of cancer cells through fluid shear stress

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Embodiment Construction

[0031] The specific implementation of the present invention will be further described below in conjunction with the accompanying drawings and examples, but the implementation and protection of the present invention are not limited thereto. It should be pointed out that, if there are any processes in the following that are not specifically described in detail, those skilled in the art can realize or understand with reference to the prior art. The reagents or instruments used were not indicated by the manufacturer, and they were regarded as conventional products that can be purchased from the market.

[0032] Such as figure 1 As shown, it is a microfluidic chip device in which fluid shear force induces transmembrane migration of tumor cells provided by the present invention. The device includes a syringe pump 1 , a three-way valve 2 , a microfluidic chip 3 , an inverted microscope 4 , a silicone tube and a liquid reservoir 5 . The syringe pump is connected to a three-way valv...

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Abstract

The invention discloses a micro-fluidic chip device for inducing transmembrane migration of cancer cells through fluid shear stress. The device can simulate the true blood flow environment of the human body, and transmembrane migration behaviors of the cells under mechanical conditions are studied. The device comprises an injection pump, a three-way valve, a micro-fluidic chip, an inverted microscope, a silicone tube and a liquid storage apparatus. The injection pump is connected with the three-way valve, the three-way valve is connected with the micro-fluidic chip, the micro-fluidic chip is connected with the liquid storage apparatus, and the inverted microscope is used for observing the migration of the cells in channels of the micro-fluidic chip; the micro-fluidic chip comprises the upper and lower channels simulating the blood vessel and a thin film in the middle. The invention further provides a processing method of the micro-fluidic chip. The provided chip device has the advantages of being easy to manufacture, small in size, capable of precisely controlling the magnitude of shear stress and achieving dynamic monitoring and the like, and thus a feasible tool is provided for studying the effect of the fluid shear stress on tumor transfer.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a microfluidic chip device in which fluid shear force induces transmembrane migration of tumor cells. Background technique [0002] Metastasis is a complex and multi-step process, and almost 90% of cancer patients die from tumor metastasis. More and more evidences show that in addition to genetic and external factors, the tumor microenvironment, especially its physical factors (mechanical force, matrix stiffness, shear stress), plays an important role in the process of tumor development and metastasis. At the primary tumor site, some cancer cells undergo epithelial-mesenchymal transition, thereby gaining the ability to migrate. Cancer cells then secrete matrix metalloproteinases to degrade the extracellular matrix and then enter the blood circulation system. In the blood circulation, cancer cells will be exposed to fluid shear force, and a small part will...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/34
CPCC12M23/16C12M35/04C12M41/36
Inventor 李趣欢刘云峰吴建华方颖
Owner SOUTH CHINA UNIV OF TECH
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