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A kind of blueberry anthocyanin-chitooligosaccharide complex and its preparation method and application

A technology of blueberry anthocyanins and chitosan oligosaccharides is applied in the directions of preparation, application, and sugar derivatives of sugar derivatives, which can solve problems such as poor stability of blueberry anthocyanins, and achieve the effects of improving stability and broad application prospects.

Active Publication Date: 2022-05-03
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of poor stability of blueberry anthocyanins in the traditional processing process, the invention provides a preparation method of blueberry anthocyanins-chitooligosaccharide complex

Method used

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  • A kind of blueberry anthocyanin-chitooligosaccharide complex and its preparation method and application
  • A kind of blueberry anthocyanin-chitooligosaccharide complex and its preparation method and application
  • A kind of blueberry anthocyanin-chitooligosaccharide complex and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0061]First of all, the frozen blueberries are selected full, free of pests and diseases and mechanical damage, rinsed with clean water, placed in a low temperature thaw at 4 °C, put into the juicer to press and crush, ready to weigh the pulp 5g, add the volume fraction of 60% hydrochloric acid acid acidated ethanol solution with a volume-to-liquid ratio of 1:10, adjust the pH value of 3, ultrasonic oscillation 90min auxiliary extraction (set power is 500W), extraction temperature is 40 °C, collect filtrate, and concentrate under reduced pressure at room temperature to obtain red crude extract. The above obtained crude extract solution was purified by AB-8 macroporous resin. AB-8 macroporous resin is activated with anhydrous ethanol for 24 hours, so that it is swelled, gently stirred with a glass rod to remove the surface of the resin, repeated washing with distilled water until there is no alcohol taste, soaked with 3% hydrochloric acid for 1d, then soaked with 3% NaOH solution ...

Embodiment 2

[0067]First of all, the frozen blueberries are selected full, free of pests and diseases and mechanical damage, rinsed with clean water, placed in a low temperature thawing at 4 ° C, put into the juicer to press and crush, ready to weigh the pulp 5g, add 5g of the liquid to the material-liquid ratio into the volume fraction of 50% of the hydrochloric acid acid acidated ethanol solution, adjust the pH value of 2, ultrasonic oscillation for 60min auxiliary extraction (set power is 500W), the extraction temperature is 35 ° C, collect the filtrate, and concentrate the red crude extract solution under reduced pressure at room temperature. The above obtained crude extract solution was purified by D101 macroporous resin. D101 macroporous resin is activated with anhydrous ethyl alcohol for 24 hours, so that it is swollen, gently stirred with a glass rod to remove the surface of the resin, re-washed with distilled water until there is no alcohol taste, soaked with 3% hydrochloric acid for...

Embodiment 3

[0073]First of all, the frozen blueberries with full, pest-free and mechanical damage are selected, rinsed with clean water, placed in a low temperature thawing at 4 ° C, put them into the juicer to press and crush, ready to weigh the pulp 5g, add the volume-to-liquid ratio of 1:15 added to the volume fraction of 70% of the hydrochloric acid acid acidated ethanol solution, adjust the pH value of 4, ultrasonic oscillation 120min auxiliary extraction (set power is 500W), extraction temperature is 45 ° C, collect filtrate, reduce pressure at room temperature to obtain red crude extract. The above obtained crude extract solution is purified by X-5 macroporous resin. X-5 macroporous resin is activated with anhydrous ethyl alcohol for 24 hours, so that it is swelled, gently stirred with a glass rod to remove the resin on the surface, re-washed with distilled water until there is no alcohol taste, soaked with 3% hydrochloric acid for 1d, then soaked with 3% NaOH solution for 1d, and the...

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Abstract

The invention relates to a blueberry anthocyanin-chitooligosaccharide complex and a preparation method and application thereof, belonging to the field of food processing. A preparation method of a blueberry anthocyanin-chitooligosaccharide complex, comprising the following process steps: uniformly mixing a blueberry anthocyanin solution and a chitooligosaccharide solution to obtain a mixed solution, adding glutaraldehyde to the mixed solution, and reacting to obtain blueberry anthocyanin-chitooligosaccharide complex solution, dried to obtain blueberry anthocyanin-chitooligosaccharide complex solid powder. According to the method of the present invention, the blueberry anthocyanin extracted from the natural fruit blueberry is effectively compounded with the animal by-product shrimp and crab shell enzymatic hydrolysis product chitosan oligosaccharide. Natural anti-tumor compounds are used in functional foods and have potential research value in the medical field.

Description

Technical field [0001] The present invention relates to a blueberry anthocyanin - chitosan oligosaccharide complex and preparation method and application thereof, belonging to the food processing field. Background [0002] Blueberry is a bilberry in the rhododendron family, originating in North America, a perennial shrub small berries tree. There are more than 400 species of bilberry plants distributed throughout the world, mainly produced in the United States and also known as American blueberries. Wild blueberries in China are mainly produced in the Changbai Mountains, Daxing'anling and Xiaoxing'anling forest areas, and most of them are in the Daxing'anling area. In recent years, artificial domestication and cultivation have been successfully carried out, and China's research on blueberries began in the early 1980s, when the Jilin Agricultural University Small Berries Research Institute took the lead in entering the field of blueberry research, and was the first to establish a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K31/702A61K31/7016A61K31/722A61P35/00C12P19/26C12P19/14C07H17/065C07H1/08A23L33/105A23L33/125
CPCA61K31/7048A61K31/702A61K31/7016A61K31/722A61P35/00C12P19/26C12P19/14C07H17/065C07H1/08A23L33/105A23L33/125A23V2002/00A61K2300/00A23V2200/308A23V2250/21A23V2250/2132A23V2250/511A23V2250/28
Inventor 李斌薛博刘瑞海孟宪军张野郎宇曦冉旭龙谢旭藏志焕王维生
Owner SHENYANG AGRI UNIV