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Grape fruit ripening related gene VvNAC and application thereof

A fruit and grape technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problem of lack of understanding of molecular regulation mechanism, achieve great application value and promote fruit ripening effect.

Active Publication Date: 2019-08-16
HENAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with this type of climacteric fruit, the molecular regulation mechanism of non-climactic fruit ripening is even less understood

Method used

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  • Grape fruit ripening related gene VvNAC and application thereof
  • Grape fruit ripening related gene VvNAC and application thereof
  • Grape fruit ripening related gene VvNAC and application thereof

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Embodiment 1

[0108] In this embodiment, the recombinant expression vector contains the grape fruit ripening-related gene VvNAC, and the nucleotide sequence of the grape fruit ripening-related gene VvNAC is shown in SEQ ID NO.1.

[0109] Example 1 of the preparation method of recombinant expression vector

[0110] Construction of Overexpression Vector of Grape Fruit Ripening Related Gene VvNAC

[0111]In order to study the function of the grape fruit ripening-related gene VvNAC, a 1002bp ORF fragment including the VvNAC gene coding region was correctly inserted into the plant overexpression vector pCAMBIA2300.

[0112] According to the VvNAC gene sequence cloned in Example 1 of the grape ripening-related gene VvNAC, the upstream and downstream primers VvNAC-ORF-F and VvNAC-ORF-R that can amplify the VvNAC gene ORF were designed:

[0113] VvNAC-ORF-F: 5'-ATGGGTGTACCGGAGACTGAC-3' (as shown in SEQ ID NO.9);

[0114] VvNAC-ORF-R: 5'-TTACTGCCTATATCCAAATCCAC-3' (shown in SEQ ID NO.10).

[0115...

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Abstract

The invention relates to a grape fruit ripening related gene VvNAC and an application thereof and belongs to the technical field of plant genetic engineering. In order to analyze the molecular mechanism of grape fruit ripening and clone key genes related to fruit ripening, the fruit of Jingxiu grape is sequenced by transcriptome at different developmental stages, a significant increase in the expression of the gene is found during the conversion period, and the gene has a conversed NAC domain and is named as VvNAC. The gene VvNAC is transformed into strawberries by agrobacterium tumefaciens mediated cotyledon transformation method, and the fruit ripening stage of transgenic plants is 6-8 days earlier; the results show that the gene VvNAC and a recombinant expression vector is of great value for promoting fruit ripening and regulating the marketing time of the fruit.

Description

technical field [0001] The invention relates to a grape fruit ripening related gene VvNAC and an application thereof, belonging to the technical field of plant genetic engineering. Background technique [0002] Grape is a kind of perennial fruit tree, which is widely cultivated and utilized all over the world. It is mainly used for fresh food, juice making, drying and wine making, etc., which can create huge economic value. In addition to delicious taste, grapes are also rich in beneficial substances such as vitamins, minerals and flavonoids, which have health care functions. Resveratrol in grapes has anti-cancer, anti-oxidation and anti-inflammatory functions, and is beneficial to human health. Resveratrol is also a plant bioprotectin, which can be synthesized by plants themselves during environmental stress and pathogen invasion. Now more and more people are involved in the research of grapes. It has been confirmed that resveratrol in grapes can not only improve the resis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/74
CPCC07K14/415C12N15/8261
Inventor 余义和郭大龙李红英孟祥轩杨英军张国海
Owner HENAN UNIV OF SCI & TECH
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