Liquid aerosol lung delivery immunity mouse model of botulinum neurotoxin AHc vaccine

Abstract

The invention discloses a liquid aerosol lung delivery immunity mouse model of a botulinum neurotoxin AHc vaccine. The effective component of an A type botulinum neurotoxin vaccine provided by the invention is aerosol of a specific solution, wherein the specific solution contains specific protein and a mucosal immunity adjuvant, the specific protein is AHc protein or fusion protein having the AHcprotein, the mass compounding ratio of the specific protein to the mucosal immunity adjuvant is 1 to (0.5-1.5), and the AHc protein is the protein as shown in a sequence 1 of a sequence table. A recombinant protein AHc subunit vaccine is selected, a lung delivery immunity manner is used for the first time, and in vivo efficacy and in vitro efficacy of the AHc subunit vaccine are researched. The vaccine can protect a mouse from being attacked by a deadly A type botulinum neurotoxin. Lung delivery is performed through trachea, so that samples can directly reach the lung target organ, the effectsare direct, medicines are saved, and a new technical means is provided for inhalation infection and treatment.

Description

technical field [0001] The invention relates to a liquid aerosol lung delivery immune mouse model of botulinum toxin AHc vaccine. Background technique [0002] Botulinum neurotoxin (BoNT) is a neuroexotoxin produced by Clostridium botulinum in an anaerobic environment, and it is a highly toxic protein toxoid. Botulinum toxin is a typical type A-B neuroexotoxin. By inhibiting the release of acetylcholine vesicles at the neuromuscular junction, it blocks the impulse conduction at the neuromuscular junction, eventually leading to skeletal muscle paralysis and death from respiratory failure. It is the most toxic drug known. strong protein. [0003] Botulinum toxin is a double-chain structure consisting of a light chain (50 kDa) and a heavy chain (100 kDa), linked by disulfide bonds. Light chains are toxic components. The heavy chain is the toxicity receptor of nerve cells, which is not active itself, and is necessary for mediating the transmembrane transport of toxins and ent...

AI Technical Summary

Problems solved by technology

At present, the research on liquid aerosol or dry powder aerosol mostly uses mouse mouth and nose exposure and whole body exposure models. Although these two methods are non-invasive to experimental animals, they cannot accurately quantify the dosage, and the samples are lost in the exposure tower. Large and prone to subsidence

Moreover, the related instruments and equipment are bulky and expensive.

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