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Tissue culture and rapid propagation method of populus lasiocarpa

A technology of poplar large leaf and rapid propagation, applied in the field of plant tissue culture, can solve problems such as difficulty in preservation of poplar large leaf germplasm resources, and achieve the effects of improving the germination rate of axillary buds and improving the germination rate.

Active Publication Date: 2019-08-23
CHINESE ACAD OF FORESTRY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All of these have caused some difficulties in the preservation of the germplasm resources of Populus bigifolia

Method used

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  • Tissue culture and rapid propagation method of populus lasiocarpa
  • Tissue culture and rapid propagation method of populus lasiocarpa
  • Tissue culture and rapid propagation method of populus lasiocarpa

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Take the old leaves of Populus japonicus collected in October, remove the old leaves, and put them in a refrigerator at 4°C for 2 weeks to make the axillary buds dormant.

[0049] Soak the refrigerated poplar branches at room temperature for 2 days, then carry out hydroponics, change the water every day, wait until the axillary buds break dormancy and germinate, and when the new branchlets grow to 8-10cm (see attached figure 1 shown), cut off the twigs and soaked them in detergent solution for 10 minutes, took them out and rinsed them with running water for 1 hour, rinsed them with distilled water for 3 times, and then sterilized them in an ultra-clean workbench.

[0050] In the ultra-clean bench, put the fresh young shoots of Populus macrophyllum after preliminary cleaning in a beaker, disinfect with 70% alcohol by volume for 30 seconds, rinse with sterile water for 3 times, and soak for 5 minutes with 5% sodium hypochlorite solution, during which time Stir constantly,...

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Abstract

The invention provides a tissue culture and rapid propagation method of populus lasiocarpa, and belongs to the technical field of plant tissue culture. The tissue culture and rapid propagation methodincludes subjecting branches of the populus lasiocarpa to refrigerating, soaking and water planting in sequence to obtain tender twigs 8-10cm in length; sterilizing the tender twigs to obtain sterileleaves; cutting the sterile leaves into small pieces, and inoculating the small pieces to an adventitious bud inducing medium for inducing culture to obtain adventitious buds; cutting the adventitiousbuds into stems with axillary buds, and inoculating each stem to a rooting medium for rooting culture to obtain rooted seedlings; transplanting the rooted seedlings after domestication to obtain populus lasiocarpa seedlings. The tissue culture and rapid propagation method of the populus lasiocarpa has the advantages that the steps are simple, the cycle is short, the annual propagation number of the leaves can reach 81, and tissue culture and rapid propagation of the populus lasiocarpa can be realized.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of poplar. Background technique [0002] Populus lasiocarpa (scientific name: Populus lasiocarpa Oliv.) is a perennial woody plant of the genus Populus in the family Salicaceae. It is a native tree species unique to my country. It is native to central China and only has wild species in Hubei, Shaanxi, Sichuan, Guizhou and Yunnan provinces. Distribution, there is no wild population distribution abroad. It is also called Western Hubei Daye Poplar because it is distributed most in Northwest Hubei and Southwest Hubei. The suitable altitude of poplar is 600-2800m. It likes humid, warm and cool climate. It has a certain ability of cold resistance and high ecological value. Its adaptability in alpine areas is an excellent genetic resource for mountain poplar breeding. Its wood is white, light and soft, and has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘妍婧王一鸣曾庆银
Owner CHINESE ACAD OF FORESTRY
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