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A method and application of green preparation of nano-silver from Aspergillus japonicum fermentation broth

A fermented liquid and nano-silver technology, applied in the direction of nanotechnology, nanotechnology, nanotechnology for materials and surface science, etc., to achieve the effect of simple preparation process, broad-spectrum antibacterial activity, and broad application prospects

Active Publication Date: 2022-03-22
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, in the research of using green methods to synthesize nano-silver, most of them use plant extracts, lysozyme, amylase, etc., and there are few reports on the use of mold crude enzyme solution to generate nano-silver. Therefore, this study can be used for the preparation of nano-silver. method provides another new way of thinking

Method used

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  • A method and application of green preparation of nano-silver from Aspergillus japonicum fermentation broth
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  • A method and application of green preparation of nano-silver from Aspergillus japonicum fermentation broth

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Embodiment 1

[0026] The A.japonicus PJ01 fermentation crude enzyme liquid method for synthesizing nano-silver of the present embodiment comprises the following steps:

[0027] (1) After the fermentation, add distilled water with a solid-to-liquid ratio of 1:15, shake for 45 minutes, and filter and collect the filtrate as crude enzyme liquid;

[0028] (2) Take 10mL of crude enzyme solution in a 50mL Erlenmeyer flask and place it under magnetic stirring;

[0029] (3) When the temperature of the crude enzyme solution reaches 30°C, add 1mL of 0.8mol / L silver nitrate solution and stir evenly;

[0030] (4) Add 4 mL of 1.5 mol / L sodium hydroxide solution and fully react for 1 min;

[0031] (5) Take the reacted liquid and centrifuge at a high speed, the centrifugation condition is 10000rpm, the centrifugation time is 20min, take its precipitate, add an equal amount of distilled water for ultrasonication, and repeat the centrifugation twice;

[0032] (6) drying the precipitated product obtained i...

Embodiment 2

[0036] This embodiment is roughly the same as Example 1, except that the concentration of silver nitrate (step (3)) is different, being respectively 0, 0.2, 0.4, 0.6, 0.8, 1.0 mol / L.

Embodiment 3

[0038] This embodiment is roughly the same as Example 1, except that the concentration of sodium hydroxide (step (4)) is different, being respectively 0, 0.5, 1.0, 1.5, 1.75, 2.0 mol / L.

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Abstract

The invention discloses a method for preparing nano-silver particles with fermented liquid of Aspergillus japonicus PJ01, belonging to the field of nano-materials. The invention uses the crude enzyme liquid produced by solid-state fermentation as a reducing agent and a stabilizer, and assists in synthesizing nano-silver through simple magnetic stirring to obtain a brown liquid. The liquid is subjected to high-speed centrifugation, washing and drying to obtain powdered silver nanoparticles. Under the scanning of ultraviolet spectrum, the solution has the maximum absorption peak near 400nm. The prepared silver nanoparticles are full and spherical with good dispersion. In addition, the invention also provides the application of silver nanoparticles synthesized by enzyme solution in the antibacterial field. The production process of the invention is simple and environmentally friendly, and has broad prospects in the field of antibacterial medicine.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and in particular relates to a method for synthesizing nanosilver by producing crude enzyme liquid through solid-state fermentation of Aspergillus japonicus PJ01 strain and its application. Background technique [0002] Due to the significant increase in disease and death caused by bacterial or viral infections, and the gradual increase in resistance to traditional antibiotics and drug treatments, the preparation of non-toxic and highly resistant antibacterial drugs has become a research hotspot. Nano-silver has broad-spectrum and high-efficiency antibacterial properties, long-lasting effects, no drug resistance, environmental protection and non-toxicity, and has greater advantages than traditional antibiotics. The main preparation methods of nano-silver are chemical method, physical method and biological method, etc. The most commonly used organic solvents and reducing agents in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24A61K33/38A61P31/04B82Y30/00
CPCB22F9/24A61K33/38A61P31/04B82Y30/00B22F2009/245
Inventor 李培骏陶丽君单杨李海云李霞梁金叶杨岱峻雷丽嫦
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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