Method of constructing electrochemical aptamer sensor with titanium dioxide nanorod composite as electrochemical tracer agent

A nanocomposite material and aptamer sensor technology, which is applied in scientific instruments, analytical materials, and material analysis through electromagnetic means, can solve the problems of not meeting at the same time, Pt shedding, loss of electrocatalyst activity, etc., and reach the detection limit Low, fast detection, excellent stability effect

Inactive Publication Date: 2019-08-23
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, conventional carbon-based nanomaterial supports may not simultaneously satisfy these characteristics
For example, the instability of the catalyst support may lead to the detachment of Pt from the support, resulting in the loss of electrocatalyst activity.

Method used

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  • Method of constructing electrochemical aptamer sensor with titanium dioxide nanorod composite as electrochemical tracer agent
  • Method of constructing electrochemical aptamer sensor with titanium dioxide nanorod composite as electrochemical tracer agent
  • Method of constructing electrochemical aptamer sensor with titanium dioxide nanorod composite as electrochemical tracer agent

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Embodiment 1

[0047] A TiO 2 The nanorod composite material is a method for constructing an electrochemical aptamer sensor for directional loading supports and traceable markers, comprising the following steps:

[0048] 1) TiO 2 Preparation of nanorods:

[0049] Accurately measure 19 mL of secondary water with a clean measuring cylinder and pour it into a 100 mL small beaker. With vigorous stirring, add 1.8 mL of TiCl 4 Slowly add it dropwise to the above small beaker (ice-water bath 0-5°C), and stir vigorously for 20 min to obtain a white suspension. Add 1.8 mL of chloroform to the above mixed solution, continue to stir for 30 min, transfer to a 100 mL polytetrafluoroethylene-lined autoclave, and react at 160 °C for 12 h. Cool to room temperature, wash with deionized water and / or absolute ethanol until the pH is 7.0, collect the resulting product, and dry at 60°C overnight.

[0050] 2) Pt-Sn@TiO 2 Preparation of nanocomposites:

[0051] First, 0.19 g K 2 PtCl 6 and 0.044 g SnCl 2...

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Abstract

The invention relates to a method of constructing an electrochemical aptamer sensor with a TiO2 nanorod composite as an electrochemical tracer agent. The method comprises steps of: depositing Pt-Sn dual-metal nanoparticles on the surface of a cylindrical TiO2 nanorod uniformly through an alcohol reduction method and synthesizing a PtSn@TiO2 nano composite; dispersing the PtSn@TiO2 nano composite in a chitosan solution and then dripping the solution onto the surface of a GCE electrode, fixing NH2-cDNA on the surface of the modified electrode with BS<3> as a diamino crosslinker, and then fixingstreptomycin aptamer on the electrode through DNA hybridization of NH2-cDNA and the aptamer. When a mixture of streptomycin and exonuclease is introduced, the aptamer can combine with streptomycin specificity preferentially and separate from NH2-cDNA on the surface of the electrode so as to form a single-chain DNA structure. Exonuclease acting on the single-chain DNA alone digests and degrades single-chain NH2-cDNA, aptamer-streptomycin compound selectively, so as to enable the compound to release streptomycin, and released streptomycin can combine with remaining aptamer specificity on the surface of the electrode circularly. The electrochemical aptamer sensor can detect streptomycin rapidly and sensitively, and has a large linear range and low detection limit.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of electrochemical aptamer sensor construction, in particular to a TiO 2 The nanorod composite material is a construction method of an electrochemical aptamer sensor of an electrochemical tracer, and the electrochemical aptasensor can be used to detect streptomycin. [0003] Background technique: [0004] Streptomycin is one of the aminoglycoside antibiotics used to treat Gram-negative infections in humans and animals. However, streptomycin residues in soil, crops, and ecological environments are known to cause serious public health concerns, such as allergic reactions, nephrotoxicity, and ototoxicity. For example, ototoxicity can cause damage to the eighth cranial nerve, leading to dizziness, tinnitus, hearing loss, or permanent deafness. Conventional streptomycin analysis methods include radioimmunoassay, enzyme-linked immunosorbent assay, high-performance liquid chromatography, and liquid chromat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 刘小强李乐乐杨立威唐云飞张思郑贺杰
Owner HENAN UNIVERSITY
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