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Injectable antimicrobial peptide hydrogel and preparation method thereof

An antibacterial peptide and hydrogel technology, applied in the field of injectable antibacterial peptide hydrogel and its preparation, can solve the problems of large toxic and side effects, inability to achieve injectability, poor antibacterial effect of polypeptide hydrogel, etc., and achieve good antibacterial effect. Properties, moderate strength, viscosity reduction effect

Active Publication Date: 2019-08-27
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention proposes an injectable antimicrobial peptide hydrogel and its preparation method, which solves the problems that the existing polypeptide hydrogel has poor specific antibacterial effect, cannot be injected, and has serious side effects

Method used

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  • Injectable antimicrobial peptide hydrogel and preparation method thereof
  • Injectable antimicrobial peptide hydrogel and preparation method thereof
  • Injectable antimicrobial peptide hydrogel and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of injectable antimicrobial peptide hydrogel, the process flow is shown in figure 2 ,Specific steps are as follows:

[0034] (1) Preparation of antimicrobial peptides

[0035] The sequence of the antimicrobial peptide is Ac-RKKWFW-NH 2 , containing 3 hydrophobic amino acids and 3 hydrophilic amino acids, conforming to the typical structural characteristics of antimicrobial peptides. Ac-RKKWFW-NH 2 Antibacterial peptides use Rink amide-AM resin as a solid phase carrier, L-type amino acids protected by 9-fluorenylmethoxycarbonyl: Fmoc-Phe-OH, Fmoc-Trp(Boc)-OH, Fmoc-Lys(Boc)-OH, Fmoc -Arg(Pbf)-OH is amino acid raw material, 1-hydroxybenzotriazole (HOBt), O-benzotriazole-tetramethyluronium hexafluorophosphate (HBTU), diisopropylethylamine ( DIEA) is the catalyst, DMF is the solvent, and the solid-phase synthesis technology is used to prepare according to the polypeptide sequence. Among them, the active site of the solid-phase carrier, amino acid ra...

Embodiment 2

[0039] A preparation method of injectable antimicrobial peptide hydrogel, the process flow is shown in figure 2 ,Specific steps are as follows:

[0040] (1) Preparation of antimicrobial peptides

[0041] The sequence of the antimicrobial peptide is Ac-RKKWFW-NH 2 , containing 3 hydrophobic amino acids and 3 hydrophilic amino acids, conforming to the typical structural characteristics of antimicrobial peptides. Ac-RKKWFW-NH 2Antibacterial peptides use Rink amide-AM resin as a solid phase carrier, L-type amino acids protected by 9-fluorenylmethoxycarbonyl: Fmoc-Phe-OH, Fmoc-Trp(Boc)-OH, Fmoc-Lys(Boc)-OH, Fmoc -Arg(Pbf)-OH is amino acid raw material, 1-hydroxybenzotriazole (HOBt), O-benzotriazole-tetramethyluronium hexafluorophosphate (HBTU), diisopropylethylamine ( DIEA) is the catalyst, DMF is the solvent, and the solid-phase synthesis technology is used to prepare according to the polypeptide sequence. Among them, the active site of the solid-phase carrier, amino acid raw...

Embodiment 3

[0045] A preparation method of injectable antimicrobial peptide hydrogel, the process flow is shown in figure 2 ,Specific steps are as follows:

[0046] (1) Preparation of antimicrobial peptides

[0047] The sequence of the antimicrobial peptide is Ac-RKKWFW-NH 2 , containing 3 hydrophobic amino acids and 3 hydrophilic amino acids, conforming to the typical structural characteristics of antimicrobial peptides. Ac-RKKWFW-NH 2 Antibacterial peptides use Rink amide-AM resin as a solid phase carrier, L-type amino acids protected by 9-fluorenylmethoxycarbonyl: Fmoc-Phe-OH, Fmoc-Trp(Boc)-OH, Fmoc-Lys(Boc)-OH, Fmoc -Arg(Pbf)-OH is amino acid raw material, 1-hydroxybenzotriazole (HOBt), O-benzotriazole-tetramethyluronium hexafluorophosphate (HBTU), diisopropylethylamine ( DIEA) is the catalyst, DMF is the solvent, and the solid-phase synthesis technology is used to prepare according to the polypeptide sequence. Among them, the active site of the solid-phase carrier, amino acid ra...

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Abstract

The invention relates to the field of antibacterial hydrogel, in particular to injectable antimicrobial peptide hydrogel and a preparation method thereof. The invention solves the problems that the specific antibacterial effect is poor, injection cannot be achieved, and toxic and side effects are severe of existing polypeptide hydrogel. The hydrogel is prepared by the self-assembly of antimicrobial peptides. According to the injectable antimicrobial peptide hydrogel and the preparation method thereof provided by the invention, the small-molecule antimicrobial peptides are taken as raw materials, the antimicrobial peptides can destroy microbial membranes, the antibacterial effect is generated, and therefore, the addition of antibacterial drugs is not needed; the hydrogel is prepared by a self-assembly mechanism, the addition of cross-linking agents is not needed, and the self-assembly can be completed within several minutes. The prepared hydrogel is moderate in strength and shows the shear thinning effect; the syringeability is realized; the hydrogel can be applied to deep tissues; and the prepared hydrogel has better antibacterial properties.

Description

technical field [0001] The invention relates to the field of antibacterial hydrogel, in particular to an injectable antibacterial peptide hydrogel and a preparation method thereof. Background technique [0002] In the 1970s, hydrogel antibacterial dressings began to be developed by European and American countries. Compared with traditional dressings, hydrogel dressings have a higher water content, so the wound will not scab and heal faster. The main components of hydrogel dressings are organic and polymer materials, so they are easily degraded and excreted in the body. Most of the hydrogel dressings currently on the market are prepared by combining antibacterial polymers or antibacterial agents with polymers, and their antibacterial properties mainly come from the polymer matrix or antibacterial agents. [0003] Antimicrobial peptides are active molecules produced by biological organisms in nature to fight bacterial infections. In recent years, antimicrobial peptides have...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K38/08A61P31/04C07K7/06C07K1/06C07K1/04
CPCA61K38/08A61K9/06A61K9/0024A61P31/04C07K7/06Y02P20/55
Inventor 曹凤仪梅林
Owner ZHONGYUAN ENGINEERING COLLEGE
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