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Directional culture medium of germinating seeds of oreorchis patens(Lindl.)Lindl. and directional culture and seedling method

A technology for germinating seeds and medium, which is applied in the field of directional medium for germinating seeds and directional cultivation of orchid seedlings to achieve the effects of shortening the time for seedlings and improving the efficiency of seedlings

Active Publication Date: 2019-08-30
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no technology that adopts the germinated seeds of orchid as explants to carry out directional culture into seedlings

Method used

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  • Directional culture medium of germinating seeds of oreorchis patens(Lindl.)Lindl. and directional culture and seedling method
  • Directional culture medium of germinating seeds of oreorchis patens(Lindl.)Lindl. and directional culture and seedling method
  • Directional culture medium of germinating seeds of oreorchis patens(Lindl.)Lindl. and directional culture and seedling method

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Experimental program
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Embodiment 1

[0041] 1 Test materials and methods

[0042] 1.1 Test material

[0043] The germinated seeds that can be distinguished by naked eyes are used as explants (about 1*1*1mm) for the protocorm proliferation test, the proliferated protocorms are used as explants for bulb differentiation, and the corms produced by the differentiation of protocorms are used as explants for rooting test .

[0044] 1.2 Effect of mitogens on protocorm proliferation

[0045]MS+NAA0.1mg / L+sucrose 30g / L+agar powder 4.5g / L was used as the basic medium, and mitogens were added as TDZ, Zt, 6-BA, and KT, and the addition amounts were 0.1mg / L and 2.0mg / L , 4.0mg / L, 6.0mg / L, 8.0mg / L, 10.0mg / L, the culture temperature was 23°C, the light-dark cycle was 14h / 10h, the light intensity was 1500lx, and the culture time was 55d. Each treatment was repeated 3 times. Repeat 15 explants, and the statistical index is the average weight of the explants.

[0046] 1.3 Effect of auxin on protocorm proliferation

[0047] MS+...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a directional culture medium of germinating seeds of oreorchis patens(Lindl.)Lindl. and a directional culture and seedling method. The directional culture medium is prepared from a proliferation culture medium which is an MS culture medium containing sucrose, agar powder, 6-BA and NAA, a differentiation culture mediumwhich is an MS culture medium containing sucrose, agar powder, KT and IBA and a rooting culture medium which is an MS culture medium containing sucrose, agar powder, 6-BA and IAA. Accordingly, the germinating seeds of oreorchis patens(Lindl.)Lindl. serve as experimental materials, the regulation and promotion effects of different mitogens and auxins and ratios on the development process from thegerminating seeds to seedlings are studied, the results show that the 6-BA and the NAA are beneficial to protocorm proliferation, the KT and the IBA are beneficial to differentiation of protocorm intocorm, and the 6-BA and the IAA are beneficial to corm rooting. According to the method, the germinating seeds of oreorchis patens(Lindl.)Lindl. grow directionally, the seedling time is shortened, andthe seedling efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a directional culture medium for germinated orchid seeds and a method for directional cultivation of seedlings. Background technique [0002] Oreorchis patens (Lindl.) Lindl. is a perennial shade herbaceous plant of Orchidaceae Oreorchis. It is widely distributed in Southwest my country, Central China, Northwest China and Northeast China. It also has a small amount of distribution in the Korean Peninsula, Siberia and Japan. Its dried corm is used as medicine, sweet, pungent, cold, slightly poisonous. It has the effects of nourishing yin and clearing the lung, resolving phlegm and relieving cough, and is used for carbuncle rickets, sores, scrofula, and unknown swelling. However, the medicinal value of Shanlan has not been valued for a long time, but it has been treated as a counterfeit of Shanzi mushroom or used as a customary medicine. [0003] In recent years, with the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张正海张亚玉孙海张悦
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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