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Lactic acid bacterium composite agent for reducing toxicity of phoxim to eriocheir sinensis, and preparation method thereof

A compound agent and lactic acid bacteria technology, applied in the field of biochemistry, can solve problems such as strong toxicity and potential safety hazards, and achieve the effects of fewer production processes, improved quality, and simple production processes

Active Publication Date: 2019-08-30
苏州宏螯生物农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some problems have also been found, mainly due to the use of pesticides to control insects more or less in the long-term planting process of rice fields in the past. The residues of these pesticides used in the past in the soil are toxic to aquaculture animals such as Chinese mitten crabs Stronger, resulting in new security risks

Method used

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  • Lactic acid bacterium composite agent for reducing toxicity of phoxim to eriocheir sinensis, and preparation method thereof
  • Lactic acid bacterium composite agent for reducing toxicity of phoxim to eriocheir sinensis, and preparation method thereof
  • Lactic acid bacterium composite agent for reducing toxicity of phoxim to eriocheir sinensis, and preparation method thereof

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Experimental program
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Effect test

Embodiment 1～4

[0057] Adopt the following method to prepare the lactic acid bacteria compound provided by the invention according to the mass percent of each component in table 1 and the concentration of live bacteria respectively:

[0058] Table 1

[0059]

[0060] The preparation steps of the lactic acid bacteria compound agent used in embodiment 1~4 are as follows:

[0061](1) Preparation of Lactobacillus bulgaricus powder: under sterile conditions, inoculate the Lactobacillus bulgaricus strain into the liquid medium, place it in a constant temperature shaker at 37°C for 18 hours, and then put the activated Lactobacillus gasseri strain into In a fermenter, carry out secondary fermentation for 36 hours at 28°C according to 5% inoculum, adjust the pH to 4 with citric acid, and freeze-dry to obtain Lactobacillus bulgaricus powder. The formula of the fermentation medium is (mass fraction) soluble starch 37 %, soybean meal 30%, fish meal 20%, molasses 10%, sodium chloride 3%.

[0062] (2)...

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Abstract

The present invention discloses a lactic acid bacterium composite agent for reducing toxicity of phoxim to eriocheir sinensis and a preparation method thereof. The lactic acid bacterium composite agent comprises the following components: lactobacillus bulgaricus, lactobacillus acidophilus, bacillus subtilis, an enzyme and a buffer agent. A total viable bacterium concentration of the lactobacillusbulgaricus, lactobacillus acidophilus and bacillus subtilis is larger than or equal to 10 billion / g. The lactic acid bacterium composite agent can effectively reduce the toxicity of phoxim residues ina comprehensive rice breeding system on the eriocheir sinensis, improves a survival rate of the eriocheir sinensis, reduces toxicity risks, improves breeding efficiency of the eriocheir sinensis, andenhances quality of the eriocheir sinensis.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to a lactic acid bacteria compound agent, in particular to a lactic acid bacteria compound agent for reducing the toxicity of phoxim to Chinese mitten crabs and a preparation method thereof. Background technique [0002] Chinese mitten crab (Eriocheirsinensis), also known as river crab, is an important economic crab for farming. In recent years, the breeding mode of Chinese mitten crab has been continuously improved and developed, and the breeding output is also increasing year by year. In 2014, the production of Chinese mitten crab hepatopancreas was as high as 796,500 tons, with a total output value of about 50 billion yuan. Chinese mitten crab farming has become an effective means to increase employment levels and improve people's quality of life. [0003] The rice field comprehensive planting and breeding technology combines rice planting and Chinese mitten crab culture, overlaps the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18A23K20/189A23K20/26A23K20/20C12N1/20C12R1/225C12R1/23C12R1/125
CPCA23K50/80A23K10/18A23K20/189A23K20/26A23K20/30C12N1/20A23V2400/123A23V2400/113
Inventor 胡鲲陈献练小军
Owner 苏州宏螯生物农业发展有限公司