Application of flora abundance testing sample to preparing nasal polyp and prognostic testing agent thereof

A technology for detecting substances and nasal polyps, which is applied in the fields of biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc. Whether the effect of recurrence

Active Publication Date: 2019-09-10
BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More importantly, these indicators detected by nasal polyp samples are mostly used to predict the curative effect after nasal endoscopy, and have little significance in guiding the selection of preoperative drug treatment and surgical options

Method used

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  • Application of flora abundance testing sample to preparing nasal polyp and prognostic testing agent thereof
  • Application of flora abundance testing sample to preparing nasal polyp and prognostic testing agent thereof
  • Application of flora abundance testing sample to preparing nasal polyp and prognostic testing agent thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1. Research object

[0068] In this study, 22 controls and 66 CRSwNP patients (including 25 eosinophilic nasal polyps, 14 neutrophil nasal polyps, and 13 non-eosinophilic / non-neutrophil nasal polyps) were collected from Beijing Tongren Hospital. Nasal polyps, 14 mixed nasal polyps) with nasal cotton swabs (Copan, Brescia, Italy). The control group was derived from patients with anatomical variation undergoing septal deviation correction but without any other nasal disease.

[0069] Clinical data were collected for all subjects, including gender, age, comorbid diseases (asthma, allergic rhinitis, specificity), preoperative symptom scores (smell, nasal congestion, runny nose, head and face pain, sneezing, and preoperative total score). points), baseline histopathological cell count percentage (eosinophils, neutrophils, lymphocytes, plasma cells) and preoperative peripheral blood inflammatory cell percentages (eosinophils, neutrophils, lymphocytes) .

[0070] 2. Sample ...

Embodiment 2

[0087] The relationship between flora and polyp recurrence

[0088] Study on recurrence included all nasal polyp samples

[0089] 1. There was no significant difference in flora richness and uniformity between recurrent and non-recurrent nasal polyps, but there were significant differences in the entire flora structure ( Figure 14-16 ).

[0090] 2. There are differences in the abundance of flora at the phylum level and genus level between relapse and non-relapse ( Figure 17-18 ). The recurrence group had more phylum Proteobacteria, genus Prevotella, Fusobacterium, Clostridium sensustricto and Veillonella, less phylum Actinobacteria, genus Corynebacterium, Staphylococcus, Propionibacterium and Anaerococcus.

[0091] 3. The flora can predict the recurrence of nasal polyps

[0092] Integrating different clinical indicators and differential flora into Logistic regression analysis, we found that only tissue eosinophils % (the area under the curve (AUC) = 0.908) and phylum Act...

Embodiment 3

[0095] A method for screening bacterial flora to predict recurrence

[0096] Among the 66 patients with nasal polyps, except for one who was lost to follow-up, the remaining 65 patients had undergone standardized follow-up and medication, and had complete clinical data. Among them, there were 31 people in the recurrence group and 34 people in the non-relapse group.

[0097] 1. Single factor analysis

[0098] The 17 clinical indicators before operation and the relative abundance of the top 5 phylum levels and the top 30 genera level bacteria obtained by 16s rDNA sequencing were compared between the recurrence group and the non-relapse group, and a statistical P value less than 0.05 was regarded as statistically significant. difference.

[0099] The 17 clinical indicators are: age, gender, combined asthma, combined allergic rhinitis, combined atopy, preoperative symptom score (smell, nasal congestion, runny nose, head and face pain, sneezing and the total score of preoperative...

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Abstract

The invention relates to the field of nasal polyp and prognostic testing thereof, in particular to application of a flora abundance testing sample to preparing nasal polyp and a prognostic testing agent thereof. By comparing the differences of the compositions and structures of paranasal sinus microorganisms between CRSwNP and a healthy contrast, especially evaluating the relationship between thecompositions of the paranasal sinus microorganisms and the inflammation type, whether the composition of a certain paranasal sinus microorganism is related to the postoperative polyp relapse of a CRSwNP patient, and the synergistic prediction value of inflammation cells and different bacteria on the polyp relapse is further evaluated.

Description

technical field [0001] The invention relates to the field of detection of nasal polyps and their prognosis, and in particular relates to the application of a substance for detecting the abundance of flora in the preparation of a detection agent for nasal polyp typing and its prognosis. Background technique [0002] Chronic rhinosinusitis with nasal polyps (CRSwNP) is a complex and heterogeneous sinus mucosal inflammation characterized by the presence of nasal polyps estimated to occur in 1% to 4% of the population worldwide. In Europeans and Americans, the immunological features of CRSwNP are mainly Th2 inflammation; while in Asian countries, such as China and Korea, at least half of CRSwNP patients show Th1 / Th17 inflammation. Inflammation typing is based on the proportion of inflammatory cells that infiltrate the tissue, with eosinophilia being one of the most critical features. Th2 inflammation is associated with recurrence of nasal polyps. Eosinophils are the most effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/689C12Q1/04C12N15/11
CPCC12Q1/6883C12Q1/689C12Q2600/112C12Q2600/118Y02A50/30
Inventor 张罗王成硕闫冰
Owner BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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