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Preparation of dual-modal nanoprobes and their labeling and imaging of mesenchymal stem cells

A nanoprobe, dual-modality technology, applied in the biological field, can solve problems such as quenching and hinder application, achieve good biocompatibility, good low toxicity, and achieve the effect of fluorescence and nuclear magnetic dual-modality imaging

Active Publication Date: 2022-01-21
SHANXI MEDICAL UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, conventional dyes tend to undergo photobleaching and aggregation-induced quenching after incorporation in high concentrations of silica nanoparticles, which hinders their further application in bioimaging.

Method used

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  • Preparation of dual-modal nanoprobes and their labeling and imaging of mesenchymal stem cells
  • Preparation of dual-modal nanoprobes and their labeling and imaging of mesenchymal stem cells
  • Preparation of dual-modal nanoprobes and their labeling and imaging of mesenchymal stem cells

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Embodiment Construction

[0021] Preparation of bimodal nanoprobes and their labeling for mesenchymal stem cells

[0022] First, 0.1-1 gram of mesoporous silica is calcined into fine powder at high temperature in a muffle furnace, and then compounded with 30% gadolinium. This process is achieved by adding 0.06-0.6 grams of gadolinium nitrate hexahydrate [Gd(NO 3 ) 3 ·6H 2 O] The solid was dissolved in deionized water to form a solution, and then the solution was added dropwise to the mesoporous silica powder, mixed and then dried and calcined at high temperature to obtain Gd@SiO 2 Complex;

[0023] Step 2: Take 0.02-0.2 grams of aggregation-induced emission (AIE) fluorescent dye and ultrasonically dissolve it in 300-2000 microliters of acetonitrile solution, and synthesize 0.08-0.7 grams of Gd@SiO 2 Put the composite material into a watch glass and add the AIE solution drop by drop, stir and mix it evenly, and then dry it. Then take 0.05-0.5 grams of the dried compound, add 0.25-2.5 grams of polyv...

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Abstract

The invention relates to the field of biotechnology, and relates to a method for synthesizing dual-mode nanoprobes, wherein mesoporous silica is calcined into silica fine powder, and Gd(NO 3 ) 3 ·6H 2 O dissolved in deionized water to form Gd(NO 3 ) 3 solution, and then Gd(NO 3 ) 3 The solution was added dropwise to the fine silica powder, and the dropping process was stirred, then dried and calcined to obtain Gd@SiO 2 Composite; AIE‑Gd@SiO prepared by the method of the present invention 2 The nanocomposite has good biocompatibility and low toxicity, and can be used as a nanoprobe to label mesenchymal stem cells. At the same time, it can realize dual-modal imaging of fluorescence and NMR, and is applied to the experimental research of stem cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the preparation of dual-mode nanoprobes AIE-Gd@SiO 2 , which is used to label mesenchymal stem cells to realize dual-modal imaging of stem cells by fluorescence and NMR. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells derived from the mesoderm in early development, with biological characteristics such as multilineage differentiation potential, improvement and repair of damaged tissues, immune regulation, and support for hematopoietic function. In a suitable environment, they can differentiate into osteoblasts, chondrocytes, cardiomyocytes and adipocytes. A large number of experimental studies have shown that there is a large degree of plasticity between stem cells differentiated into osteoblasts and adipocytes, and the two can transform into each other. This suggests that inhibiting adipogenic differentiation and increasing osteogeni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N24/08
CPCG01N21/6458G01N24/08
Inventor 张宏田梅张冰程艳王静
Owner SHANXI MEDICAL UNIV