Fecal calprotectin or lactoferrin combined detection reagent and preparation method thereof

Abstract

The invention discloses a fecal calprotectin or lactoferrin combined detection reagent and a preparation method thereof. The reagent comprises a PVC bottom plate, wherein a sample pad, a colloidal gold labeled pad, a nitrocellulose membrane detection pad and a water absorption pad are sequentially arranged at the top of the PVC bottom plate from left to right; a lactoferrin detection line, a calprotectin detection line and a quality control line are sequentially arranged on the internal surface of the nitrocellulose membrane detection pad from left to right; and the left side of the colloidal gold labeled pad is located on the inner side of the sample pad. The reagent can completely reflect intestinal inflammation, is used for auxiliary identification and diagnosis for distinguishing an inflammatory bowel disease and an irritable bowel syndrome, is used for early-stage screening of organic lesions such as intestinal tumors and polyps, is used for identifying acute bacterial diarrhea and nonbacterial diarrhea, can guide pharmacy, can avoid antibiotic abuse, is simple in detection technology, good in repeatability, stable in finished product structure, excellent in service performance and high in clinic practicability, and can partially replace the current invasive detection means.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a combined detection reagent for fecal calprotectin or lactoferrin and a preparation method thereof. Background technique [0002] Calprotectin is a heterodimeric protein with a molecular weight of 36kDa that binds to calcium and zinc. Related studies are increasing. At present, it is believed that the functions of calprotectin are mainly related to immune regulation, induction of cell apoptosis, and inhibition of bacterial growth, while calcium The research on the relationship between protective protein and tumor has gradually become a hot spot; lactoferrin belongs to the ferritin family, it is a secreted glycoprotein with a molecular weight of 80kDa, lactoferrin has the functions of iron metabolism, antibacterial, antiviral, immune regulation and Anti-inflammatory, anti-tumor and other biological functions, in recent years, it has been found that lactoferrin has obvio...

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Problems solved by technology

[0004] Existing studies have shown that both calprotectin and lactoferrin are involved in inflammatory response and tumor immunity, and have a wide range of biological activities. However, in the process of organic diseases such as intestinal tumors and ulcers, bleeding and A large number of inflammatory cells infiltrate, therefore, the calprotectin and lactoferrin in the patient's feces are si

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