Computer-automatic generation of digital pathological slides from he staining to ihc staining
A technology for automatic generation of digital pathological slices, applied in the computer field, can solve the problems of increasing the time cost and preparation cost of inspectors, affecting the comparison and analysis of doctors' images, and achieving the effect of shortening generation time, reducing the number of calculations, and high computing efficiency
Active Publication Date: 2020-12-25
创新工场(北京)企业管理股份有限公司
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Problems solved by technology
[0007] (1) increase the time cost and preparation cost of the tester;
[0008] (2) There are also differences in the structure and morphology of different thin-layer slices, which will affect the comparative analysis of different stained images by doctors
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[0047] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.
[0048] Such as figure 1 with figure 2 As shown, the digital pathological section of the embodiment of the present invention is automatically generated by computer from hematoxylin-eosin HE staining to immunohistochemical IHC staining, including the following steps:
[0049] In step S1, the original HE-stained pathological section image of the examiner is obtained, and the HE-stained pathological section image is preprocessed to obtain an effective HE-stained tissue area.
[0050]Pathological slices are usually stored in a mult...
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The invention provides a computer automatic generation method of a digital pathological section from HE staining to IHC staining, which comprises the following steps: S1, acquiring an original HE staining pathological section image of a detector, and preprocessing the HE staining pathological section image to obtain an HE staining effective tissue region; S2, performing gridding processing on the HE staining effective tissue area, and mapping the HE staining effective tissue area to the original HE staining pathological section image to obtain a plurality of HE image small blocks containing the effective tissue area; S3, sending each HE image small block to a trained generator, and generating a plurality of corresponding IHC dyed image blocks; and S4, splicing and assembling the plurality of IHC staining image blocks to obtain an IHC staining pathological image. According to the method, IHC dyeing prediction can be automatically generated by a computer under the condition that only HE dyeing digital sections exist, and quantitative or semi-quantitative pre-judgment is more accurately conducted in advance for a detector under the condition that IHC dyeing experiment preparation does not exist.
Description
technical field [0001] The invention relates to the field of computer technology, in particular to a method for automatic computer generation of digital pathological slides from HE staining to IHC staining. Background technique [0002] Pathological section refers to taking a certain size of lesion tissue and making pathological section with histopathological method. Lesions are usually embedded in paraffin blocks, cut into thin sections with a microtome, stained with hematoxylin-eosin (H-E), and further examined using a microscope. [0003] HE staining is used to stain the nucleus and cytoplasm with hematoxylin and eosin, which can distinguish the morphology of general cells. In practical applications, abnormal pathological changes such as tissue cell necrosis, edema, degeneration and inflammatory cell infiltration can be identified. [0004] IHC staining is to use the primary antibody to bind the target protein antigen in the detected tissue, then combine the HRP and othe...
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IPC IPC(8): G06T7/00G06T7/194G06T3/40
CPCG06T3/4038G06T7/0012G06T2207/10056G06T2207/20081G06T2207/20084G06T2207/30004G06T7/194
Inventor 张晓璐罗杰坚李晶
Owner 创新工场(北京)企业管理股份有限公司