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Method for preparing red algae phycoerythrin

A technology for red algae and algae, which is applied in the preparation methods of peptides, chemical instruments and methods, algae/moss peptides, etc., can solve the problems of long time, high cost, low yield and the like, and achieves a simple method and high recovery rate. , the effect of high purity

Inactive Publication Date: 2019-09-20
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional extraction methods include physical crushing method (ultrasonic crushing, liquid nitrogen grinding, repeated freezing and thawing), chemical osmosis method (cell swelling method, CaCl 2 solution extraction method), two-phase extraction method, flocculation method, etc., due to the high cost, long time-consuming and low yield of these methods, the industrial production is limited
Enzymatic hydrolysis is an effective alternative method to promote the destruction of algae cell tissue to release compounds. As early as 2008, Joubert and Fleurence used xylanase and cellulase to conduct research, and confirmed the feasibility of enzymatic recovery of PE. Publication number CN 101617784A also A method for the extraction of phycobiliproteins in laver using cellulase and pectinase has been published, but there is no report on the application of agarase-assisted extraction of PE

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] (1) Pretreatment: The dried laver is pulverized with a pulverizer, and passed through an 80-mesh sieve to obtain dry laver powder for later use.

[0008] (2) Fully mix the laver powder in step (1) with 0.1M phosphate buffer solution with pH 6.8-7.2 at a ratio of 1:30 to obtain a mixed solution for later use.

[0009] (3) Add 0.02% β-agarase and 0.02% cellulase in sequence to the cell suspension obtained in step (2), and enzymolyze at 30°C for 5 h under low-speed stirring to obtain a wall-breaking solution.

[0010] (4) Refrigerate and centrifuge the porphyra wall-broken liquid obtained in step (3) at 8000 r / min for 10 min to obtain the supernatant.

[0011] (5) Add ammonium sulfate with a saturation of 50% to the supernatant in step (4), let it stand overnight at low temperature, then refrigerate and centrifuge at 8000 r / min for 10 min, take the precipitate and add 0.1 pH 6.8-7.2 M Phosphate buffer reconstituted.

[0012] (6) Concentrate the protein solution reconstit...

Embodiment 2

[0015] (1) Pretreatment: Wash the fresh algal bodies with water, drain and set aside.

[0016] (2) Homogenize fresh algae from step (1) with 0.1M phosphate buffer at pH 6.8-7.2 at a ratio of 1:2-3 for later use.

[0017] (3) Add 0.01% β-agarase and 0.01% cellulase to the cell suspension obtained in step (2) in sequence, and enzymatically hydrolyze at 30°C for 5 h under low-speed stirring to obtain a wall-breaking solution.

[0018] (4) The porphyra wall-broken liquid obtained in step (3) was centrifuged at 8000 r / min for 10 min at low temperature to obtain the supernatant.

[0019] (5) Concentrate the supernatant in step (4) to 1 / 5 of the original volume, add ammonium sulfate with a saturation of 50%, let stand overnight at low temperature, and then refrigerate and centrifuge at 8000 r / min After 10 min, the precipitate was taken and redissolved in 0.1M phosphate buffer with pH 6.8-7.2.

[0020] (6) Concentrate the protein solution reconstituted in step (5) by ultrafiltration...

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Abstract

The invention provides a method for extracting PE from red algae, which is simple in operation, low in cost and suitable for industrial production. The method selects beta-agarase and cellulase to extract phycoerythrin from marine red algae, saves time, reduces cost, greatly simplifies operation, has high recovery rate and high purity by comparing with the prior art, and provides an easy way to industrialize phycoerythrin production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for extracting phycoerythrin in red algae by using agarase. Background technique [0002] Rhodophyta refer to the algal plants of the Rhodophyta. Among the red algae, there are some nutritious and delicious edible species such as seaweed, Eucheuma, seaweed, etc.; there are also some important economic species used to extract agar and carrageenan, such as agaricus, Gracilaria, wrinkle Plants such as carrageen and Eucheuma are mostly multicellular, and a few are unicellular. There are only Rhodophyceae in this phylum, with about 760 genera and more than 4,410 species. Rhodophyceae is divided into two subclasses: Porphyra and True Rhodophyceae; red algae are rich in biologically active substances (proteins, polyunsaturated fatty acids and polysaccharides) and have good biological activities (antioxidant, anti-inflammatory , antibacterial and anti-tumor), the development and util...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/405C07K1/36C07K1/34C07K1/30
CPCC07K14/405
Inventor 江晓路王纯
Owner OCEAN UNIV OF CHINA
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