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Acute detecting method for microorganisms in packed drinking water

A sensitive detection and microbial technology, which is applied in the field of sensitive detection of Pseudomonas aeruginosa in packaged drinking water, can solve the problems that the detection results cannot be fed back in real time and guide production, the time-consuming of primary screening is short, and the time-consuming of primary screening is long. Achieve the effects of narrowing the focus range, increasing sensitivity, and improving accuracy

Pending Publication Date: 2019-09-24
CHINA RESOURCES CESTBON BEVERAGE CHINA CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems of the existing packaged drinking water microorganisms such as Pseudomonas aeruginosa detection method that the initial screening takes a long time to determine, and the detection results cannot be fed back in real time to guide production, etc., and provides a wide application range, high detection sensitivity, and time-consuming initial screening and determination. Short microbial (especially Pseudomonas aeruginosa) detection method

Method used

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  • Acute detecting method for microorganisms in packed drinking water
  • Acute detecting method for microorganisms in packed drinking water
  • Acute detecting method for microorganisms in packed drinking water

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Sensitive detection of Pseudomonas aeruginosa in packaged drinking water

[0025] Using the filter membrane separation method, filter 250mL of water samples with a 0.45μm filter membrane, and transfer the filter membrane to 50mL CN liquid medium, and culture at a constant temperature of 36±1°C for 8-24h with shaking at a speed of 150rpm. The water samples include test samples and sterile water used as a negative control sample.

[0026] Since the CN liquid medium contains material components that make it have a certain background turbidity, first use a turbidity meter to measure the bulk turbidity value of several CN liquid medium and calculate the average turbidity value to establish the culture of CN liquid medium Liquid background turbidity quality control table (such as shown in Table 1 below), to determine its background turbidity range value, used to compare the turbidity value of the negative control sample with the average turbidity value, to judge whether the n...

Embodiment 2

[0035] Sensitive detection of bacteria in packaged drinking water

[0036] Using the filter membrane separation method, 250mL water sample was filtered with a 0.45μm filter membrane, and the filter membrane was transferred to 50mL TSB liquid medium, and incubated at a constant temperature of 36±1°C for 8-24h with a rotation speed of 150rpm. The water samples include test samples and sterile water used as a negative control sample.

[0037] Take out the cultured negative control and test samples, and directly observe the turbidity of the negative control samples and test samples with the naked eye. If the negative control sample is clear and not turbid, showing no microbial reproduction, then it is determined that the negative control sample is an effective negative control sample.

[0038] Directly observe and compare the turbidity of the test sample and the effective negative control sample by naked eyes. If the turbidity of the test sample is significantly greater than the...

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Abstract

The invention relates to the technical field of detection of microorganisms in drinking water, in particular to an acute detecting method for microorganisms in packed drinking water, which is particularly suitable for detection of pseudomonas aeruginosa in packed drinking water. Through the manners of a filtration membrane separation method, a centrifugal separation method and the like, potential microorganisms in detection samples are effectively separated and inoculated to a liquid nutrient medium, and through combination with a constant-temperature shaking culture manner, the detection sensitivity is improved; after culture for 8-24h, an optical detection method can be used for objectively distinguishing the turbidity difference of a culture solution, so that decision for primary screening results can be made, and the microbiological contamination risk of products can be assessed. A manufacturing enterprise can efficiently use feedback of the microorganism detection results of the products for guiding actual production.

Description

technical field [0001] The invention relates to the technical field of detection of microorganisms in drinking water, in particular to a sensitive detection method for Pseudomonas aeruginosa in packaged drinking water. Background technique [0002] Microbial contamination is an important factor affecting food safety, and it is also the main reason for unqualified packaged drinking water products. Microbiological testing of the production process and finished products is a necessary means of risk control. [0003] Pseudomonas aeruginosa is a key indicator bacterium for quality control in the production process of packaged drinking water, and the detection ability of Pseudomonas aeruginosa is directly related to the guarantee of product quality. The "Detection method for Pseudomonas aeruginosa" in GB 8538-2016 "Examination Methods for Drinking Natural Mineral Water" is the current national standard method for the detection of Pseudomonas aeruginosa in packaged water. Cultivat...

Claims

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Application Information

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IPC IPC(8): C12Q1/04G01N15/06
CPCC12Q1/04G01N15/06G01N15/075
Inventor 罗慧琳张志刚钟华林
Owner CHINA RESOURCES CESTBON BEVERAGE CHINA CO LTD
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