A preparation method of hydrogen-accumulated nanobubble resistant starch
A technology of resistant starch and nanobubbles, which is applied in the direction of gas-containing food ingredients, the function of food ingredients, and food ingredients as antioxidants. It can solve the problems of poor hydrogen enrichment and retention, difficulty in ensuring safety, and low utilization efficiency. To achieve the effect of broadening effective development channels, improving utilization efficiency, and removing malignant free radicals
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Embodiment 1
[0026] First, the starch granules are regulated by enzymatic hydrolysis of high-temperature α-amylase in hydrostatic water (the reaction temperature is 100°C, the amount of water added is 3 times the mass of the starch, the content of the enzyme is 5% of the mass of the starch granules, and the reaction time is 12 hours), forming a nanosheet layered matrix The porous resistant starch precursor of the wall, the gap between the nanosheets is 30nm, and the starch is subjected to secondary hydrophobic modification after deactivation, and the hydrophobic group used is the grafting group—C 6 h 5 , the interlayer gap of the hydrophobically modified starch crystallization layer is 10nm, after which ultrasonic oscillation (temperature 20°C) generates hydrogen-accumulated nanobubble resistant starch, and 2% of the hydrogen-rich starch is added to fruit juice and drunk.
[0027] The hydrogen-rich starch prepared above was added to the hydrophobic dye rhodamine 6G to reversely mark hydrog...
Embodiment 2
[0029] First, the starch granules are regulated by enzymatic hydrolysis of α-amylase and glucoamylase in still water (reaction temperature is 70°C, the amount of water added is 3 times the mass of starch, the content of enzyme is 2% of the mass of starch granules, and the stirring reaction time is 24h). The porous resistant starch precursor of the nanosheet layered matrix wall, the gap between the nanosheets is 60nm, and the starch is subjected to secondary hydrophobic modification after enzyme inactivation, and the hydrophobic group used is the grafting group—C=CH 2 , the interlayer gap of the starch crystalline layer after hydrophobic modification is 30nm, after which ultrasonic oscillation (temperature 0°C) generates hydrogen-accumulated nanobubble resistant starch, and 6% of the hydrogen-rich starch is added to milk for drinking.
[0030] The hydrogen-rich starch prepared above was added to the hydrophobic dye rhodamine 6G to reversely mark hydrogen, and the distribution wa...
Embodiment 3
[0032]First, the starch granules are regulated by enzymatic hydrolysis of α-amylase and glucoamylase in still water (reaction temperature is 70°C, water addition is 3 times the starch mass, enzyme content is 4% of the starch granule mass, and the stirring reaction time is 24h), forming The porous resistant starch precursor of the nanosheet layered matrix wall, the gap between the nanosheets is 60nm, and the starch is subjected to secondary hydrophobic modification after enzyme inactivation. The hydrophobic group used is the grafting group—Cl. After hydrophobic modification The interlayer gap of the starch crystallization layer is 30nm, after which the hydrogen-rich nanobubble resistant starch is produced through ultrasonic oscillation (temperature 0° C.), and 8% of the hydrogen-rich starch is added to milk and drunk.
[0033] The hydrogen-rich starch prepared above was added to the hydrophobic dye rhodamine 6G to reversely mark hydrogen, and the distribution was observed under ...
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