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Fluorescence detection method of antiepileptic drug --- tiagabine hydrochloride (tgb)

A technology of tiagabine hydrochloride and anti-epileptic drug, applied in the field of fluorescence detection of anti-epileptic drug tiagabine hydrochloride, can solve the problems of high analysis cost, complicated analysis process, long detection time, etc., and achieve good selectivity , high sensitivity, convenient and fast detection process

Active Publication Date: 2022-04-22
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

The detection of TGB by high performance liquid chromatography is a classic detection method with good linearity and high accuracy, but this method also has some shortcomings and deficiencies, such as: high analysis cost, complicated analysis process, long detection time, etc.

Method used

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  • Fluorescence detection method of antiepileptic drug --- tiagabine hydrochloride (tgb)
  • Fluorescence detection method of antiepileptic drug --- tiagabine hydrochloride (tgb)
  • Fluorescence detection method of antiepileptic drug --- tiagabine hydrochloride (tgb)

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Embodiment

[0031] An antiepileptic drug of the present invention --- fluorescence detection method of thiogalbine hydrochloride (TGB), comprising the following steps:

[0032] (1) Establish a fluorescent probe --- PDI-HSA

[0033] PDI and HSA were dissolved in 50% ethanol solution and ultrapure water, respectively, and both were prepared into a 1 mM mother liquor. Take a certain volume of the above two mother liquors and dilute them into concentrations of 5 μM and 40 μM respectively to obtain fluorescent probe PDI-HSA. This includes the following steps:

[0034] (1) Take a certain volume of the above PDI mother liquor diluted with ultrapure water to 20 μM, and measure the maximum excitation wavelength of PDI by ultraviolet absorption spectroscopy.

[0035] (2) Take a certain volume of PDI mother liquor diluted with ultrapure water to 5 μM, measure the fluorescence intensity by the fluorescence spectrometer at the maximum excitation wavelength, and then add a series of HSA solutions of differ...

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Abstract

The invention discloses a fluorescence detection method for antiepileptic drug tiagabine hydrochloride (TGB). Through the study of the interaction of dye perylene diimide derivatives (PDI), human serum albumin (HSA) and tiagabine hydrochloride (TGB), we found that the fluorescence of PDI can be quenched by HSA, and our research It shows that PDI binds at site II of HSA, and TGB can compete for binding at site II of HSA, so that PDI is competed from site II of HSA, thereby realizing fluorescence recovery. Based on this, the present invention proposes a fluorescence detection method for TGB, which uses PDI‑HSA as a probe to realize fluorescence recovery under the action of TGB, so as to detect TGB. The method of the invention has the advantages of original innovation, simple detection process, mild experimental conditions, high sensitivity, good selectivity, fast detection time, low detection limit, etc., and has good social value and application prospect.

Description

Technical field [0001] The present invention belongs to the field of drug detection, in particular to an antiepileptic drug --- fluorescence detection method of thiogalbine hydrochloride (TGB). Background [0002] Epilepsy is one of the most common diseases of the nervous system, the main clinical manifestations are: during the seizure, there are different degrees of disorders in sensory, motor, behavior and autonomic nerves, which seriously threaten human health and the quality of life of patients. At present, the most important and commonly used treatment for epilepsy is still drug therapy. Tigabine hydrochloride (TGB) is a new type of anti-epileptic drug whose mechanism of action is to block the reuptake of γ-aminobutyric acid (GABA) by neurons and glial cells, increase the level of synaptic site γ-aminobutyric acid (GABA), and achieve anticonvulsant effect. However, studies have shown that excessive use of TGB can produce a series of side effects, such as dizziness, nervousn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 邹振廖小豆杨荣华闫奇杨华
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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