Fluorescent reporter molecule for high throughput screening of OGT inhibitor

Abstract

The invention, which relates to the field of molecular biology, provides a fluorescent reporter molecule for high throughput screening of an OGT inhibitor. The fluorescent reporter molecule comprisesa fluorescent reporter system for generating a fluorescent reporter molecule. The fluorescent reporter molecule contains a transcription factor, a reporter gene, and an internal reference gene. Therefore, a technical problem of constructing a fluorescent reporter molecule for high throughput screening of an OGT inhibitor is solved. The fluorescent reporter molecule is suitable for high throughputscreening of an OGT inhibitor.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a fluorescent reporter molecule used for high-throughput screening of OGT inhibitors. Background technique [0002] Many studies have shown that abnormal O-GlcNAc modification is closely related to the occurrence and development of various diseases including cancer. At present, most of the methods commonly used to detect O-GlcNAc modification rely on antibody-based immunohistochemical staining or western-blot detection. Although accurate, the steps are cumbersome, the sample requirements are strict, and the antibody used is expensive, so it is not suitable for O-GLcNAc modification. Batch testing and high-throughput screening of relevant inhibitors. [0003] In order to solve the above technical problems, some people introduced FRET technology on the basis of metabolic labeling method, that is, fluorescence resonance energy transfer technology, which can perform imaging and dete...

AI Technical Summary

Problems solved by technology

At present, most of the methods commonly used to detect O-GlcNAc modification rely on antibody-based immunohistochemical staining or western-blot detection. Although accurate, the steps are cumbersome, the sample requirements are strict, and the antibody used is expensive, so it is not suitable for O-GLcNAc modification. Batch detection and high-throughput screening of relevant inhibitors

[0003] In order to solve the above technical problems, some people introduced FRET technology on the basis of metabolic labeling method, that is, fluorescence resonance energy transfer technology, which can perform imaging and detection of O-GlcNAc modification, but some of these methods can only be used for extracellular detection. , some require the introduction of special compounds that are likely to cause cytotoxicity, and the applicability is not high

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