Method for polyethylene biodegradation through pichia guilliermondii

A technology of biodegradation and polyethylene, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve environmental pollution and other problems, and achieve the effect of low cost and simple operation

Active Publication Date: 2019-10-11
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem of environmental pollution caused by traditional methods

Method used

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  • Method for polyethylene biodegradation through pichia guilliermondii
  • Method for polyethylene biodegradation through pichia guilliermondii
  • Method for polyethylene biodegradation through pichia guilliermondii

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specific Embodiment approach 1

[0011] Specific embodiment one: this embodiment utilizes the method for biodegrading polyethylene by Pichia mongoliae, and proceeds according to the following steps:

[0012] 1. Activation of Pichia mongoliana strain: Scrape and inoculate Pichia mongoliana strain stored on YPD slant medium at 4°C to YPD solid medium, and culture at 30°C for 24-48 hours to obtain activation the strain;

[0013] 2. Biodegradation of polyethylene by Pichia mongolica: Scrape the activated strain with an inoculation needle, inoculate it into 80mL YPD liquid medium, and cultivate it at 30°C and 150rpm for 24-48 hours, then centrifuge at 9000rpm to collect the bacteria , washed the collected thalli with sterile normal saline for 3 times to prepare the bacterium suspension, and added chlorinated polyethylene sheets (200 mg) and Pichia mongoliana suspension (10 mL) through ultraviolet irradiation for 2 hours to In a 500mL Erlenmeyer flask with 90mL carbon-free medium (LCFBM), the final bacterial conce...

specific Embodiment approach 2

[0017] Specific embodiment two: the difference between this embodiment and specific embodiment one is: Pichia pastoris Meyerozymaguilliermondii ZJC-1 in step 1 is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, Courtyard No. 1, Beichen Road, Chaoyang District, Beijing. The preservation date is December 17, 2018, and the preservation number is CGMCC No: 16956. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0018] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: in step 1, culture at 30° C. for 36 hours. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for polyethylene biodegradation through pichia guilliermondii, in particular to a method for polyethylene biodegradation. The problems are solved that in the prior art, since lots of polyethylene products which are used are high in molecular weight, good in stability, high in surface hydrophobicity and low in surface energy, the degradation speed of the polyethylene products in the natural environment is low, and the environment pollution is caused. The method comprises the steps of 1, activation of a pichia guilliermondii strain; 2, polyethylene biodegradation through the pichia guilliermondii. The method is low in cost and easy to implement; polyethylene biodegradation can be achieved through the pichia guilliermondii, and the degradation rate of the pichia guilliermondii within 60 days reaches 13.97%. The method for polyethylene biodegradation can be applied to the fields such as plastic pollution treatment and provides a new idea for solving the problem of white pollution of plastic.

Description

technical field [0001] The invention relates to a method for biodegrading polyethylene. Background technique [0002] Polyethylene is one of the most widely used polymer synthetic materials in daily life. It feels like wax, is odorless and non-toxic, and has good stability. Except for oxidizing acids, it can withstand the erosion of most strong acids and alkalis. It is insoluble in common solvents at room temperature, has low water absorption, and has excellent electrical insulation properties and low temperature resistance. According to the survey, 72% of polyethylene plastic products are concentrated in packaging, daily use and agricultural use, but most of these plastic products are disposable products with a short service life and become waste after use. Discarded plastics are generally rarely recycled. For example, a large amount of agricultural polyethylene plastic film is generally piled up and scattered on the field after use. Due to the incomplete decomposition of ...

Claims

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Application Information

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IPC IPC(8): C12N1/16B09C1/10C12R1/84
CPCC12N1/16B09C1/105Y02W30/62
Inventor 张杰刘长莉王滨松张国财郝孔利王珊珊董欣欣姬彦飞高超王天顺刘雪
Owner NORTHEAST FORESTRY UNIVERSITY
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