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Method for identifying gnaphalium affine variety by utilizing psbA-trnH sequence

A technology for identifying Rat and Rhododendron, applied in the field of biological identification, can solve problems such as low accuracy, and achieve the effects of improving accuracy, good practical value and high stability

Active Publication Date: 2019-10-15
XIAN MEDICAL UNIV
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  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for identifying the species of S. sageii by using the psbA-trnH sequence, which solves the problem of low accuracy of the existing identification methods of S. sagebana

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  • Method for identifying gnaphalium affine variety by utilizing psbA-trnH sequence
  • Method for identifying gnaphalium affine variety by utilizing psbA-trnH sequence
  • Method for identifying gnaphalium affine variety by utilizing psbA-trnH sequence

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Embodiment Construction

[0035] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0036] A kind of method of the present invention utilizes psbA-trnH sequence identification sagera species, specifically comprises the following steps:

[0037] Step 1, pre-treating the samples of S. japonicus to be identified, and extracting the DNA of the samples of S. japonicus to be identified using the Tiangen Plant Genomic DNA Extraction Kit, specifically:

[0038] Step 1.1, pretreatment of the unidentified sagebrush sample: take 30 g of the unidentified sagebrush sample that has been dried on silica gel, grind it into powder in a liquid nitrogen environment, and set aside;

[0039] Wherein the source of the samples to be identified is as shown in Table 1 below: including the sage in different regions, number 1-9, edelweiss, number 10, sagebrush, number 11,

[0040] Table 1 Sources of S. sageri samples to be identified

[0041]

[...

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Abstract

The invention discloses a method for identifying gnaphalium affine variety by utilizing a psbA-trnH sequence. The method comprises the following steps of: pre-treating a gnaphalium affine sample to beidentified, and extracting DNA of the gnaphalium affine sample to be identified by using a kit; carrying out PCR amplification on the basis of the obtained DNA, carrying out bidirectional sequencingon a PCR product through a sequencer to obtain a sequencing result, and determining a nucleotide sequence of a psbA-trnH spacer region; and acquiring a homologous sequence of gnaphalium affine plantsand a sequence of unrelated species, annotating a corresponding psbA-trnH spacer region sequence, calculating a K2P genetic distance, constructing a phylogenetic tree, and identifying gnaphalium affine plant varieties. The method is characterized by using a spacer sequence between a psbA gene and a trnH gene. Compared with classical identification methods, the method can accurately and rapidly detect the gnaphalium affine and easily-mixed products thereof from traditional Chinese medicinal materials, improves the detection accuracy, has high stability and strong identification capability, andis suitable for identifying closely-related species and easily-mixed species samples of the gnaphalium affine.

Description

technical field [0001] The invention belongs to the technical field of biological identification methods, and in particular relates to a method for identifying sagera species by using the psbA-trnH sequence. Background technique [0002] Gnaphalium affine D.Don is an annual herb of the genus Gnaphalium affine in the family Asteraceae. The whole herb of Trichosanthes japonicus is used as medicine. Its nature is sweet, slightly sour, flat in nature, and returns to the lung meridian. It is non-toxic and has the effects of relieving cough and asthma, lowering blood pressure, and dispelling wind and cold. In the folk, sagebrush is also known as Qingmingcai and Vertus, and many scholars have developed it as a health food in modern times, such as sagebrush sake, freeze-dried sagebrush powder, health drinks, etc. [0003] There are as many as 20 species of Salmonella in my country, which are very similar in shape and difficult to distinguish. For example, sage grass and silk cotto...

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/6895G16B30/10
CPCC12Q1/6869C12Q1/6895G16B30/10C12Q2531/113Y02A50/30
Inventor 郑梦迪张寒张彦
Owner XIAN MEDICAL UNIV
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