Microfluidic device for tumor cell screening based on droplet deposit and screening method for microfluidic device

A microfluidic device and tumor cell technology, applied in the direction of measuring devices, chemical instruments and methods, and laboratory containers, can solve the problems of abnormal growth and proliferation mechanisms and low detection efficiency, and achieve high-sensitivity real-time detection, sensitivity High performance with short analysis times

Active Publication Date: 2019-10-18
KUNMING UNIV OF SCI & TECH
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Problems solved by technology

Tumor is one of the most dangerous diseases that threaten human life and health. The root cause of its occurrence is the abnormality of the mechanism of controlling cell growth and proliferation, which is related to the abnormality of cell apoptosis. The traditional medical detection methods for tumor cells have low detection efficiency. To

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  • Microfluidic device for tumor cell screening based on droplet deposit and screening method for microfluidic device
  • Microfluidic device for tumor cell screening based on droplet deposit and screening method for microfluidic device
  • Microfluidic device for tumor cell screening based on droplet deposit and screening method for microfluidic device

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Embodiment 1

[0023] Example 1: A microfluidic device for tumor cell screening based on droplet registration, including a glass substrate 1, a PDMS chip 2 fixed on it, and a controller. The PDMS chip 2 includes a continuous phase inlet 4, a focusing Structure 5, continuous phase outlet 7, dispersed phase inlet 6, register channel, outlet 3, focusing structure 5 is a cross-shaped channel, and the two inlet ports of focusing structure 5 are respectively connected to continuous phase inlet 4 and dispersed phase inlet 6 through microchannels , the two outlets are respectively connected to the continuous phase outlet 7 and the inlet of the register channel through the microchannel, the dispersed phase entering from the dispersed phase inlet 6 and the continuous phase entering from the continuous phase inlet 4 pass through the confocal structure 5 to generate encapsulating cell droplets 8. The encapsulated cell droplet 8 enters the register channel through the microchannel between the focusing str...

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Abstract

The invention relates to a microfluidic device for tumor cell screening based on droplet deposit and a screening method for the microfluidic device, and belongs to the technological field of microfluidic. The microfluidic device comprises a glass substrate and a PDMS chip fixed on the glass substrate, wherein PDMS materials constitute a whole chip. A microchannel structure is made by micro-processing technology, a droplet formed from a cross structureenvelops a red blood cell or a tumor cell, then passes through a droplet depository passive valve, due to the differences in the size and softness between red blood cells and tumor cells, the number of drops that envelop the red blood cells is different from the number of drops that envelop the tumor cells, by detecting the number of dropletspassing through laser, whether tumor cells are existed can be detected, and a high sensitivity real-time detection with very few samples can be achieved. According to the microfluidic device, the integration of fluid sampling, driving, controlling, transportation and detection analysis on a microfluidic chip can be realized, and controllable liquid flow, transport of micro and trace substances andefficient detection can be realized.

Description

technical field [0001] The invention relates to a microfluidic device and a screening method for tumor cell screening based on droplet storage, belonging to the field of microfluidic technology. Background technique [0002] Microfluidic chips are widely used in disease diagnosis, drug screening, environmental testing, food safety and other fields. One of the basic points of the microfluidic chip is to operate and control the fluid at the micro scale, and the amount of fluid as the object of operation and control is extremely small, and many manifestations of substances are different from constant substances. Tumor is one of the most dangerous diseases that threaten human life and health. The root cause of its occurrence is the abnormality of the mechanism of controlling cell growth and proliferation, which is related to the abnormality of cell apoptosis. The traditional medical detection methods for tumor cells have low detection efficiency. Sincerely. Therefore, how to q...

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Application Information

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IPC IPC(8): B01L3/00G01N15/10
CPCB01L3/502784G01N15/10B01L2200/0647B01L2200/12G01N2015/1022G01N2015/1027
Inventor 龙威刘云赵娜陈娅君魏先杰吴蜜蜜王继尧
Owner KUNMING UNIV OF SCI & TECH
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