COI primer for PCR detection of peanut aphids and application thereof

An aphid and peanut technology, applied in the agricultural field, can solve problems such as unsatisfactory specificity, and achieve the effects of increasing accuracy, enhancing ecological regulation, and strong specificity

Pending Publication Date: 2019-10-18
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The primers used to detect peanut aphids are mentioned in the prior art document "COI Gene Detection Technology of Five Types of Aphid Pests", but when the upstream and downstream primers of the primers are compared on NCBI Blast, it is found that The primers are highly similar to non-target species Hyperomyzus sp., Aphis nerii, Rhopalosiphum nymphaeae, Hyperomyzuspallidus, Acyrthosiphon sp., Tuberolachnus salignus, etc., and some of them have common species in China, so they are not specific enough for detection ideal

Method used

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  • COI primer for PCR detection of peanut aphids and application thereof
  • COI primer for PCR detection of peanut aphids and application thereof
  • COI primer for PCR detection of peanut aphids and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for detecting natural enemies of peanut aphids, comprising the following steps:

[0038] (1) Sampling Heterochromia in a peanut field, soaking the insect samples collected in the field in absolute ethanol, and storing them in a -20°C refrigerator for genome extraction and later molecular detection of intestinal contents;

[0039] (2) The genome of the Heterochromia sample was obtained by extracting the whole insect with the kit, and the quality of the extracted genome was verified by agarose gel electrophoresis. figure 1 Middle 1-3, it can be seen from the figure that the genome extraction method of Heterochromia is applicable and feasible;

[0040] (3) Use primers to carry out PCR amplification reaction to the genome of the heterochromia sample, wherein, the primer sequence is:

[0041] HS-F1:5'-GGAATAATTGGATCTTCACTTAGTATT-3' (SEQ ID No.1);

[0042] HS-R1: 5'-AAGGTAGTTCTGAATATGAATGTTCTA-3' (SEQ ID No.2);

[0043] The reaction system of the PCR amplification...

Embodiment 2

[0048] The difference between the present embodiment and embodiment 1 is that the sample is derived from the ladybug moire;

[0049] See figure 1 Middle 4-6, it can be seen from the figure that the genome extraction method of Heterochromia is applicable and feasible;

[0050] For the results of the amplification reaction, see figure 2 In 1-4, it can be seen from the figure that the genome of the ladybug sample in this example was specifically amplified in the amplification reaction, and it can be determined that the ladybug is the natural enemy of the peanut aphid, and it can eat at this stage Peanut aphids.

Embodiment 3

[0052]According to the method in Examples 1-2, this example detects the samples of Heterochromia and Moleensis in different periods in the peanut field, and the statistical results are shown in image 3 , it can be seen from the figure that both the tortoise and the heterochromia have feeding effects on peanut aphids, and the predation effects are different in different periods.

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Abstract

The invention relates to the technical field of agriculture, in particular to a COI primer for PCR detection of peanut aphids and application thereof. The primer comprises a forward primer HS-F1 and areverse primer HS-R1. The primer is high in specificity, can be used for amplifying a clear and single specific band only for the peanut aphids, and has no amplification effect on other species in the same environment with the peanut aphids. The invention further provides a method for detecting natural enemies of the peanut aphids through a PCR method. Therefore, a technical support is provided for qualitative and quantitative evaluation of the nutritional relationship between predators and prey, the control effect of various predatory natural enemies on the peanut aphids can be determined conveniently, the ecological regulation and control effects of the natural enemies on the peanut aphids can be enhanced, and the purposes of controlling pests and increasing the yield are achieved.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a COI primer for PCR detection of peanut aphids and an application thereof. Background technique [0002] Peanut is a bulk oil crop and is widely planted in China. At the same time, my country is also the largest consumer and exporter of peanut. Peanut aphids are one of the important pests that damage peanuts. This kind of pest is widely distributed in the main peanut producing areas of my country. Usually, adults and nymphs cluster on the buds, leaves and fruit needles of peanuts, often causing leaf curls. Shrinkage, yellowing, slow plant growth, and even fruit needles cannot be needled, affecting the number and development of pods; in addition, peanut aphids can also transmit peanut stripe virus (PStV) and peanut mottle virus (PMoV). Pests and diseases occurred side by side, resulting in a severe reduction in peanut production. At present, the control of peanut aphids ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2565/125
Inventor 鞠倩曲明静李晓杜龙姜晓静曲春娟
Owner SHANDONG PEANUT RES INST
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