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Method for tracing nerve fiber bundles in insect light color tentacle

A technology of nerve fiber bundles and antennae, applied in the field of tracing nerve fiber bundles in light-colored antennae of insects

Active Publication Date: 2019-10-22
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the problem in the prior art that it is difficult to trace the nerve fiber bundles in the light-colored antennae of insects, the present invention provides a method for tracing the nerve fiber bundles in the light-colored antennae of insects. Cobalt dipping, microscopic separation of living antennae and brain, color development, fixation, dehydration, transparent treatment of antennae and brain, fixed antennae posture, and microscopic photography can realize the tracing of nerve fiber bundles in the complete antennae; a breakthrough Due to the limitations of slicing techniques, the transparent technology of light-colored antennae was established, which realized the in-situ visualization of nerve fiber bundles in the antennae, and obtained fine information such as the number, spatial distribution and travel route of nerve fiber bundles in the complete antennae

Method used

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  • Method for tracing nerve fiber bundles in insect light color tentacle
  • Method for tracing nerve fiber bundles in insect light color tentacle
  • Method for tracing nerve fiber bundles in insect light color tentacle

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Example 1: The antennae of Bactrocera guava are awn-shaped, divided into stalk, stalk and flagellum, with a total length of about 860-1000 μm, the widest about 170 μm, and the narrowest about 100 μm. The shapes and sizes of each section are different. On the whole, it is a sensory organ with small size and irregular geometric shape, which integrates various functions such as smell, hearing, temperature and humidity, and gravity;

[0049] A method for tracing nerve fiber bundles in the antennae of the fruit fly Guava (see figure 1 ),Specific steps are as follows:

[0050] (1) Nickel chloride dipping of antennae: according to the size of Bactrocera japonica, select the tip of a 200 μL micro-sampler, cut off the tip and tail of the tip, and put Bactrocera japonica into the gun after freezing and anesthesia The head and tail end, and pushed to the tip of the gun head with a slight force, so that the head of the insect is fixed by the tip of the gun head and the antennae ar...

Embodiment 2

[0059]Example 2: The antennae of Bacteralis dorsalis are divided into stalk, stalk and flagellum, with a total length of about 1030 μm, wherein the stalk is about 190 μm long and 120-160 μm wide, and the peduncle is about 230 μm long and 90-170 μm wide. The peduncle is about 630 μm long, 150-178 μm wide, slightly thicker in the middle, the widest part is about 180 μm at the base of the flagellum, and the narrowest part is about 90 μm at the base of the peduncle. The size and shape of each segment are different, and the overall shape is irregular shape;

[0060] A method for tracing nerve fiber bundles in the antennae of Bactrocera dorsalis, the specific steps are as follows:

[0061] (1) Cobalt chloride impregnation of antennae: According to the size of Bactrocera dorsalis, select the tip of a 200 μL micro-sampler, cut off the tip and tail of the tip, and put Bactrocera dorsalis into the gun after freezing and anesthesia The head and tail end, and pushed to the tip of the gun...

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Abstract

The invention discloses a method for tracing nerve fiber bundles in an insect light color tentacle, and belongs to the technical field of experimental sample processing. The tracing of the nerve fiberbundle in the intact tentacle can be realized through living tentacle nickel chloride or cobalt chloride immersion, living tentacle and brain microseparation, color development, fixation, dehydration, tentacle and brain transparent treatment, tentacle posture fixation and microphotography. The problem of intact tentacle transparency and multi-angle visualization of the nerve fiber bundles under the condition of no slicing or destroying the tentacle can be solved and the connection of the nerve fiber bundles and the brain in the tentacle can be directly observed at the level of nerve pathway.The method is low in cost, reliable, easy and convenient to operate and good in reproducibility and can directly obtain the multidimensional data of the internal neural structure of the tentacle.

Description

technical field [0001] The invention relates to a method for tracing nerve fiber bundles in light-colored antennae of insects, and belongs to the technical field of experimental sample processing. Background technique [0002] Antennas are essential for insects to survive and reproduce. Insects mainly sense changes in the external environment such as smell, wind, temperature and humidity through their antennae. With the help of antennae, insects can perform a series of life activities such as finding food or mates, avoiding natural enemies, communicating within species, and finding suitable spawning grounds. According to morphological differences, the antennae of insects can be divided into bristle-like, awn-like, filamentous, and club-like. The antennae are divided into three segments: stalk, stalk, and flagellum. The surface is covered with a layer of chitinized exoskeleton, which has the functions of protecting the inner soft structure and reducing water evaporation. Th...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/30
Inventor 曹军郭凯飞代曼曹婵刘一淼杨洋胡劭骥叶辉
Owner YUNNAN UNIV
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