Oral guided bone regeneration membrane prepared from sheep periosteum acellular matrix and preparation method thereof

A decellularized matrix and guided regeneration technology, applied in the field of oral guided bone regeneration membrane and its preparation, to achieve the effects of promoting osteogenesis, promoting adhesion, and good biocompatibility

Active Publication Date: 2022-02-15
中国医科大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the relevant research focuses on how to explore more advanced biological material preparation methods, so as to simulate the artificial periosteum material that is closer to the structure and function of the human periosteum, while few studies focus on the natural periosteum itself.

Method used

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  • Oral guided bone regeneration membrane prepared from sheep periosteum acellular matrix and preparation method thereof
  • Oral guided bone regeneration membrane prepared from sheep periosteum acellular matrix and preparation method thereof
  • Oral guided bone regeneration membrane prepared from sheep periosteum acellular matrix and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Various mammalian periosteum comparative tests.

[0036] Dissect and dissect beef shanks, pig shanks, sheep shanks and rabbit shanks respectively to obtain periosteum materials. The above-mentioned 4 materials are compared, and the specific data are shown in the table below.

[0037] Table 1 Comparison data table of various mammalian periosteum

[0038]

[0039] After dissecting and comparing the periosteum of various mammals (cattle, pig, sheep, and rabbit), it is found that the sheep periosteum material is not only rich in sources and simple in the dissection process, but also its thickness and mechanical properties are more suitable for utilization. The thickness of sheep calf periosteum is moderate, which is similar to the thickness of commercial commercial membranes currently used clinically, and is more suitable for the subsequent preparation of oral-guided bone regeneration membrane. However, the periosteum of bovine and pig calf is too thick, and th...

Embodiment 2

[0041] Example 2 Preparation method and biological performance evaluation of decellularized sheep periosteum.

[0042] 1. The specific steps of decellularized sheep periosteum are as follows:

[0043](1) Obtain fresh lamb calf bone, peel off the epidermis and fascia layer by layer, completely expose the periosteum layer on the surface of the calf bone, use a scalpel to incise the periosteum around the selected periosteum area, and carefully peel off the selected area with a periosteum peeler Intact periosteal tissue, such as figure 1 shown.

[0044] (2) Store the fresh sheep periosteum at -80°C for at least 6 hours, and then thaw it in a water bath at 37°C for about 30 minutes. The above two steps constitute a freeze-thaw cycle, which is performed 3 times in total.

[0045] (3) After the last sample was thawed, the sample was placed in 1% (vol / vol) Triton X-100 and treated on a shaker at a constant speed (100 rpm) for 12 hours.

[0046] (4) The sample was placed in a 0.2% (...

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Abstract

The invention relates to the field of biomedical materials, in particular to an oral guided bone regeneration membrane prepared from amniotic periosteal acellular matrix and a preparation method thereof. The present invention adopts amniotic periosteum tissue to prepare and obtain amniotic periosteal acellular matrix guided bone regeneration membrane through raw material treatment, decellularization, sample disinfection and other processes, which is used in guided bone regeneration for repairing alveolar bone or jaw bone defect. The amniotic periosteum-derived acellular matrix guided bone regeneration membrane effectively removes the cellular components in the tissue, eliminates the immunogenicity of the xenogeneic tissue, retains the active components of the amniotic periosteal extracellular matrix, and provides a good microenvironment for bone repair , to promote the repair and reconstruction of oral bone defects. In addition, the method for preparing the sheep acellular periosteum of the present invention has low cost, and compared with other mammals, the thickness and mechanical properties of the sheep periosteum material are more suitable for the application of the oral guided bone regeneration technology.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to an oral cavity guided bone regeneration membrane prepared from sheep periosteum acellular matrix and a preparation method thereof. Background technique [0002] Implant restoration has become the first choice for patients with missing teeth, but the key to successful implant restoration is sufficient alveolar bone in the missing teeth. However, clinically, due to inflammation, trauma, disuse atrophy, jaw tumor surgery, There are a large number of cases where conventional implant restoration cannot be performed due to insufficient bone height or width in the implant area due to factors such as lack of teeth. Oral guided bone regeneration (GBR) technology originated from guided tissue regeneration (GTR) in periodontal surgery, and was officially introduced into implant surgery in the 1980s. clinical application effect. This technology is to apply a piece of biofilm material to e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
CPCA61L27/3604A61L27/3687A61L27/3691A61L27/3645A61L2430/02
Inventor 敖强何晶陶美含
Owner 中国医科大学
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