A kind of fish antifreeze protein bionic freeze-resistant sticky tough hydrogel and preparation method thereof

Abstract

The invention provides a method for preparing a low-temperature freeze-resistant sticky and tough hydrogel biomimetic of fish antifreeze protein. The low-temperature freeze-resistant sticky and tough hydrogel is obtained by introducing antifreeze protein extracted from fish into a chemically cross-linked acrylamide gel network system. Using the non-colligative properties of antifreeze proteins to lower the freezing point, inhibit recrystallization and modify the shape of ice crystals, it can effectively inhibit the freezing of water molecules in the hydrogel at low temperatures, and then endow the hydrogel with a certain degree of low temperature freezing resistance. In addition, based on the abundant amino acid groups of the antifreeze protein molecule itself, it can interact with the acrylamide chemical crosslinking network and the matrix, thus endowing the hydrogel with excellent adhesion and mechanical properties. The low-temperature freeze-resistant adhesive tough hydrogel prepared by the present invention has a sticky peeling force of up to 515.45 N / m, a tensile strength of up to 135.27 kPa, and is not frozen at -15°C, which is expected to be used as a wound dressing to protect the skin from Frostbite, broaden the application range of hydrogel.

Description

technical field [0001] The invention provides a fish antifreeze protein biomimetic freeze-resistant, sticky and tough hydrogel and a preparation method thereof, belonging to the technical field of polymer hydrogels. Background technique [0002] Hydrogel is a hydrophilic three-dimensional network polymer, which can absorb a large amount of water and has a three-dimensional network structure. Biomedicine, bionic skin and other fields have potential application prospects; however, the water molecules of traditional hydrogels will be frozen in the extreme environment of supercooling, resulting in the loss of many original excellent properties, which restricts its application scope; based on the current situation, in recent years, Recently, researchers have developed some antifreeze hydrogels, most of which are endowed with antifreeze ability by introducing organic solvents (ethylene glycol, glycerol) into the hydrogels, but these substances are often Poor biocompatibility limi...

AI Technical Summary

Problems solved by technology

[0002] Hydrogel is a kind of hydrophilic three-dimensional network polymer, which can absorb a large amount of water and has a three-dimensional network structure; due to its unique physical and chemical properties and high similarity with human tissue, it is widely used in tissue engineering, Biomedicine, bionic skin and other fields have potential application prospects; however, the water molecules of traditional hydrogels will be frozen in extreme cold environments, resulting in the loss of many original excellent properties, which restricts its application range; based on the current situation, in recent years Recently, researchers have developed some antifreeze hydrogels, most of which are endowed with antifreeze ability by introducing organic solvents (ethylene glycol, glycerol) into the hydrogel, but these substances often Poor biocompatibility limits the practical application of hydrogels; at the same time, viscous hydrogels have also been successfully prepared and used in fields such as skin repair, wound dressing and tissue adhesives; however, most of the existing viscous hydrogels Poor performance, easy to break and difficult to repair during use, greatly limiting its application