Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment

A technology of domestic sewage and yeast, applied in the field of environmental biology, can solve problems such as shortage of phosphorus resources, red tide, damage to water bodies and water ecological environment, etc., to improve treatment efficiency and stability, broaden application research ideas, and enhance practical application value Effect

Inactive Publication Date: 2019-11-12
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Phosphorus is a trace element that all life (animals, plants, microorganisms) depend on for survival, and is an important component of bacterial nucleoprotein, nucleic acid and other substances; Two-thirds of the phosphate rock is used for phosphate fertilizers, and is also used to make phosphoric acid, pesticides, washing powder, etc.; however, phosphorus is a one-way cycle of non-recyclable resources, and its consumption will cause serious losses, even worse than water and oil are more valuable; according to statistics, the annual production of phosphate rock in the world is about 100 million tons, and it will reach the peak of global phosphate rock output in 2035, after which it will face a shortage of phosphorus resources; at the same time, with the continuous development of industrial enterprises, A large amount of phospho

Method used

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  • Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment
  • Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment
  • Efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment

Examples

Experimental program
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Example Embodiment

[0019] Example 1: Isolation, screening and identification of strains.

[0020] (1) Material preparation:

[0021] 1. Collect the particulate filter material from the well-functioning A / O alternate biofilm system and prepare it into a bacterial suspension, and use the gradient dilution method to inoculate the diluted solution onto the yeast screening medium to carry out the coating and separation of the yeast; Use an inoculation loop to pick different types of yeasts for streaking purification; finally, store the purified yeasts in an ultra-low temperature refrigerator at -86℃;

[0022] 2. Medium

[0023] Yeast screening medium (1L): 20 g peptone, 20 g glucose, 10 g yeast extract, 15~20 g agar powder, 15ug / ml  streptomycin, 60 mg / L chloramphenicol, sterilized at 121 ℃ 15 min;

[0024] YPD solid medium (1L): 20g peptone, 20g glucose, 10g yeast extract, 15~20g agar powder, sterilized at 121℃ for 15min;

[0025] YPD liquid medium (1L): 20 g peptone, 20 g glucose, 10 g yeast extract, steri...

Example Embodiment

[0048] Example 2 Strains of the present invention Candida rugosa Cultivation of BL3.

[0049] (1) Optimization of the cultivation conditions of the strains of the present invention

[0050] ①Different pH

[0051] Use hydrochloric acid and sodium hydroxide to adjust pH to 3, 4, 5, 6, 7, 8, 9 (3 parallel samples) before sterilizing the wastewater culture medium, take 16 mL of bacterial suspension for inoculation, and adjust OD 600 =0.2±0.02, shake culture on a shaker (28 ℃, 220 r / min) for 60 h, measure OD 600 And PO in supernatant 4 3- -P concentration, calculate the phosphorus removal rate, see the result figure 2 ;by figure 2 It can be seen that the OD of the strain of the present invention is within the range of pH 3-9 600 , Phosphate removal rate gradually increased, when the pH value is 3, the phosphate removal rate of strain BL3 is only 40.6%; when the pH value is between 5 and 7, the phosphate removal rate of strain BL3 remains above 75%, the highest value It is 83.8% when pH...

Example Embodiment

[0060] Example 3 Strains of the present invention Candida rugosa Application of BL3 in sewage treatment: Take sewage from the regulating pond of a middle water station in a university in Shandong (COD concentration is 400mg / L, phosphorus concentration is 11mg / L, ammonia nitrogen concentration is 44 mg / L), and the wastewater phosphorus removal test is carried out, the specific steps As follows: Take 350 ml of actual sewage (sterilized) in a 500 ml Erlenmeyer flask and adjust the OD 600 =0.2±0.02, under the conditions of pH 5 and temperature 25 ℃, shake the aerobic culture on a shaker with a shaking frequency of 220 r / min; respectively at 0h, 4 h, 8 h, 12 h, 24 h, 36 h , 48 h, 60 h, take test water samples, centrifuge and take the supernatant, measure the concentration changes of orthophosphorus, ammonia nitrogen, and COD in the supernatant. Image 6 ;by Image 6 It can be seen that within 12 hours of aerobic culture, the strain of the present invention has a removal rate of 84.9%...

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Abstract

The invention relates to efficient phosphorus-removing saccharomyces and application thereof in domestic sewage treatment and belongs to the field of environmental microbiology. The saccharomyces strain is named Candida rugosa BL3 and belongs to Candida rugosa, bacterial colonies are in a light yellow waxy shape and linked by milk white filaments in the middle, and single colonies are milk white round, 2-3 mm, smooth and waxy on surface, not transparent, viscous and easy to prick up, have a strong wine aroma and can form round ascospores, pseudohypha and fungal hypha. The strain has an efficient phosphorus-removing function, requires no strict control on the dissolved oxygen environment, still has no phosphorus release phenomenon after being cultured for 60 h, and can be applied to biological sewage treatment processes. The strain has the optimum pH value range of 5-7 and growth temperature of 20-35 DEG C and adopts a mixed source of glucose and sodium acetate as the best carbon source. Actual domestic sewage treatment finds that the strain has the removal rate of phosphorus in sewage being 84.9%, the removal rate of ammonia nitrogen being 39.5% and the removal rate of COD being 78.3% under an aerobic condition at the pH value of 5 and at the temperature of 25 DEG C.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a high-efficiency phosphorus-removing yeast and its application in domestic sewage treatment. Background technique [0002] Phosphorus is a trace element that all life (animals, plants, microorganisms) depend on for survival, and is an important component of bacterial nucleoprotein, nucleic acid and other substances; Two-thirds of the phosphate rock is used for phosphate fertilizers, and is also used to make phosphoric acid, pesticides, washing powder, etc.; however, phosphorus is a one-way cycle of non-recyclable resources, and its consumption will cause serious losses, even worse than water and oil are more valuable; according to statistics, the annual production of phosphate rock in the world is about 100 million tons, and it will reach the peak of global phosphate rock output in 2035, after which it will face a shortage of phosphorus resources; at th...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/02C02F3/34C12R1/72C02F101/16C02F101/30C02F101/10
CPCC12N1/02C02F3/347C02F2101/105C02F2101/16C02F2101/30C12R2001/72C12N1/165
Inventor 邱立平胡孟飞杨炎明谢康刘贵彩孙绍芳厉鹏远高明昌邱琪程仁振
Owner UNIV OF JINAN
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