Method for preparing light calcium carbonate by using primary microorganisms

A light calcium carbonate and microorganism technology, which is applied in the field of chemical materials, can solve the problems that the impact of the natural environment is difficult to ignore, and the quality of calcium carbonate is difficult to be effectively controlled, so as to achieve the effect of improving mineralization efficiency, ensuring continuous supply, and continuous metabolic proliferation.

Active Publication Date: 2019-11-12
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to differences in production technology and equipment level, it is difficult to effectively control the quality of calcium carbonate
In addition, when using the above method to prepare calcium carbonate, a large amount of natural limestone needs to be mined, and the impact on the natural environment is hard to ignore

Method used

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  • Method for preparing light calcium carbonate by using primary microorganisms
  • Method for preparing light calcium carbonate by using primary microorganisms
  • Method for preparing light calcium carbonate by using primary microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Use a 75% alcohol-sterilized soil fetcher to collect 500 g of a sandy soil sample at a depth of 20 cm located at a certain lakeshore in the East Lake Scenic Area of ​​Wuhan City.

[0024] (2) Take a small amount of soil sample and put it into an opening at one end, and use a perforated rubber stopper at the other end to seal the plexiglass tube. Inject the growth-promoting solution into the collected soil samples continuously for 5 times with an interval of 24 hours. The formula of the growth-promoting solution is as follows :

[0025] Growth Booster Formula

[0026]

[0027] (3) Collect and filter the effluent after 5 times of growth promotion, inoculate 5ml of the filtrate into 100ml of expansion medium, culture at 30°C and 150r / min for 48h to obtain the bacterial solution for reaction, the formulation of the expansion medium is as follows:

[0028] Extended Medium Formulation

[0029]

[0030] (4) Take the bacterial liquid and the cementing liquid to reac...

Embodiment 2

[0035] (1) Aseptically collect 1000 g of aeolian sand samples from the Gurbantunggut Desert in Xinjiang, and the sampling depth is 50 cm.

[0036] (2) Put the sand sample into a Buchner funnel with a geotextile cushion, inject the growth-promoting liquid into the sand sample 7 times continuously, with a time interval of 24 hours, and the formula of the growth-promoting liquid is as follows:

[0037] Growth Booster Formula

[0038]

[0039] (3) Collect and filter the effluent after 7 times of growth promotion, inoculate 5ml of the filtrate into 100ml of expansion medium, culture at 30°C and 150r / min for 36h to obtain the bacterial solution for reaction, the formulation of the expansion medium is as follows:

[0040] Extended Medium Formulation

[0041]

[0042] (4) Take the bacterial liquid and the cementing liquid to react for 10 hours in equal volumes, wash and dry the precipitated matter after the reaction to obtain calcium carbonate, and the cementing liquid formula ...

Embodiment 3

[0047] (1) 500 g of expansive soil samples at a depth of 20 cm located somewhere in Wuhan City, Hubei Province were collected using a 75% alcohol-sterilized soil fetcher.

[0048] (2) Put the soil sample into a Buchner funnel with a geotextile cushion, and continuously inject the growth-promoting solution into the soil sample 7 times with a time interval of 24 hours. The formula of the growth-promoting solution is as follows:

[0049] Growth Booster Formula

[0050]

[0051] (3) Collect and filter the effluent after 7 times of growth promotion, inoculate 5ml of the filtrate into 100ml expansion medium, culture at 30°C and 150r / min for 24 hours to obtain the bacterial solution for reaction, the formulation of the expansion medium is as follows:

[0052] Extended Medium Formulation

[0053]

[0054] (4) Take the bacterial liquid and the cementing liquid to react for 10 hours in equal volumes, wash and dry the precipitated matter after the reaction to obtain calcium carbon...

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Abstract

The present invention discloses a method for preparing light calcium carbonate by using primary microorganisms. The method comprises the following steps: (1) collecting a soil sample; (2) preparing growth promoting liquid and cementing liquid; (3) after injecting a certain amount of the growth promoting liquid into the soil sample collected in the step (1) for a period of time, collecting effluentliquid in the soil sample; (4) inoculating the effluent liquid obtained in the step (3) into an amplification culture medium, conducting culture under a constant-temperature oscillation condition toobtain micro-organism liquid; and (5) reacting the obtained micro-organism liquid obtained in the step (4) with the cementing liquid prepared in the step (2) in an isovolumetric manner to obtain calcium carbonate. The method uses the soil treated by the growth promoting liquid to promote mass propagation of more kinds of microorganisms, then the used culture medium is mainly used for screening bacteria with urea decomposition capability, a final product of an urea decomposition reaction dependent on participation of urease microorganisms is carbonate ions, a large amount of calcium carbonate minerals can be generated by applying exogenous calcium ions, and besides, mineralization efficiency of the microorganisms can be obviously improved under synergistic effects of a plurality of the microorganisms.

Description

technical field [0001] The invention relates to the technical field of chemical materials, in particular to a method for preparing light calcium carbonate by using native microorganisms. Background technique [0002] Calcium carbonate is a high-quality and cheap inorganic filler with reinforcing or semi-reinforcing properties. It is widely used in industrial fields such as coatings, rubber, paper, medicine, food and bionic materials. At present, the industrial preparation of light calcium carbonate mostly adopts the carbonization method, that is, the carbonization reaction of calcium hydroxide suspension and carbon dioxide gas as the main raw materials, or the double decomposition reaction of water-soluble calcium salts and carbonates. However, due to differences in production technology and equipment level, it is difficult to effectively control the quality of calcium carbonate. In addition, when using the above method to prepare calcium carbonate, a large amount of natura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00
CPCC12P3/00
Inventor 张家铭周杨朱纪康
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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