A method for producing oil by co-utilizing starch and lignocellulosic raw materials
A technology of lignocellulose and starch, which is applied in the field of oleaginous microorganism cultivation, can solve the problems of complex process flow, low oil yield, and low oil yield, so as to improve fermentation efficiency, reduce process cost and equipment cost, and simplify process The effect of the process
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Embodiment 1
[0043] 1. Weigh 40g of corn stalks passed through a 40-mesh sieve, add 80mL NaOH-CH 3 OH solution, pretreatment at 80 °C for 1 h, and then continue to evaporate at the same temperature for 1 h to remove CH 3 OH;
[0044] 2. Prepare 20g / L tapioca starch and 80g / L pretreated corn stalks, control the C / N ratio to 199, adjust the pH of the oleaginous microorganism culture medium to 6.0, and sterilize at 121°C for 15 minutes to obtain starch- Lignocellulose mixed medium;
[0045] 3. Cultivate Liposaccharomyces starii AS 2.1560 (China Ordinary Microorganism Culture Collection Management Center) in seed medium (glucose 20g / L, yeast powder 10g / L, peptone 10g / L), at 28 ℃, 200rpm Vibrating culture in the environment for 40 hours to obtain oil-producing microorganism seed liquid;
[0046] 4. Inoculate the oleaginous microorganism seed solution obtained in step 3 into the starch-lignocellulose mixed medium obtained in step 2, the inoculum size is 10% (v / v), and the amount of cellulase ...
Embodiment 2
[0048] 1. Weigh 40g of corn stalks passed through a 40-mesh sieve, add 80mL NaOH-CH 3 OH solution, pretreatment at 80 °C for 1 h, and then continue to evaporate at the same temperature for 1 h to remove CH 3 OH;
[0049] 2. Prepare 50g / L tapioca starch and 150g / L pretreated corn stalk, control the C / N ratio to 300, adjust the pH of the oleaginous microorganism culture medium to 6.0, and sterilize at 121°C for 15 minutes to obtain starch- Lignocellulose mixed medium;
[0050] 3. Cultivate Liposaccharomyces starii AS 2.1560 (China Ordinary Microorganism Culture Collection Management Center) in seed medium (glucose 20g / L, yeast powder 10g / L, peptone 10g / L), at 30 ℃, 200rpm Vibrating culture in the environment for 40 hours to obtain oil-producing microorganism seed liquid;
[0051] 4. Inoculate the oleaginous microorganism seed solution obtained in step 3 into the starch-lignocellulose mixed medium obtained in step 2, the inoculum size is 15% (v / v), and the amount of cellulase ...
Embodiment 3
[0053] 1. Referring to the literature (Zhao J, Xia LM. Fuel Process Technol, 2009, 90:1193–1197), weigh 100g of straw that has passed through a 20-mesh sieve, and add 0.8L at a solid-to-liquid ratio of 1:8 (w / w). NaOH solution with a concentration of 0.5mol / L was treated in a water bath at 80°C for 75 minutes;
[0054] 2. Prepare 20g / L potato starch and 80g / L pretreated rice straw, control the C / N ratio to 88, adjust the pH of the oleaginous microorganism culture medium to 5.5, and sterilize at 121°C for 20 minutes to obtain starch- Lignocellulose mixed medium;
[0055] 3. Cultivate Liposaccharomyces starii NRRL 11558 (American Agricultural Research Culture Collection Center) in a seed medium (glucose 20g / L, yeast powder 10g / L, peptone 10g / L), in an environment of 28°C and 200rpm Cultivate under shaking for 40 hours to obtain oleaginous microorganism seed liquid;
[0056] 4. Inoculate the oleaginous microorganism seed solution obtained in step 3 into the starch-lignocellulos...
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